Genomic analysis of IMP-8-producing Enterobacter hormaechei with a novel plasmid pK432-IMP
ABSTRACT: Background: Carbapenem-resistant Enterobacter cloacae (CR-ECC) complex has posed significant challenges to the clinical treatment of infections, and Enterobacter hormaechei (E. hormaechei) is the most commonly identified nosocomial pathogen of CR-ECC. Methods: Carbapenemases were detected...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716524004429 |
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| author | Jiao Feng Zhiwei Song Piaopiao Dai Huimin Chen Dakang Hu Lianhua Yu Jin Zhang Xinhua Luo |
| author_facet | Jiao Feng Zhiwei Song Piaopiao Dai Huimin Chen Dakang Hu Lianhua Yu Jin Zhang Xinhua Luo |
| author_sort | Jiao Feng |
| collection | DOAJ |
| description | ABSTRACT: Background: Carbapenem-resistant Enterobacter cloacae (CR-ECC) complex has posed significant challenges to the clinical treatment of infections, and Enterobacter hormaechei (E. hormaechei) is the most commonly identified nosocomial pathogen of CR-ECC. Methods: Carbapenemases were detected by the immunocolloidal gold technique. The minimum inhibitory concentration (MIC) values were determined by VITEK2. The genome sequence of strain K432 was obtained and analysed. Results: We isolated the IMP-8-producing E. hormaechei strain K432 from a patient's urine specimen in a Chinese hospital, which exhibited resistance to multiple antibiotics, including ceftazidime, piperacillin/tazobactam, aztreonam, imipenem, meropenem, ciprofloxacin, levofloxacin, minocycline, and trimethoprim/sulfamethoxazole. The genome of strain K432 was composed of the chromosome cK432 (4863 kb) and 3 plasmids: pK432-IMP (45.8 kb), pK432-TEM (75.6 kb), and pK432-NR (4.8 kb). In K432, six drug-resistant genes were detected, including blaACT-7 and fosA on cK432, blaIMP-8, and qnrS1 on pK432-IMP, blaSFO-1, and blaTEM-1 on pK432-TEM. The pK432-IMP belonged to a novel incompatibility group, and pK432-TEM was an incompatibility (Inc) R plasmid. Both of these two plasmids shared similar conserved backbone regions with their reference plasmids, respectively. However, the single accessory regions in these two plasmids were different from their reference plasmids, indicating that new recombination and integration events had occurred in K432. Conclusions: This study provides a comprehensive understanding of the genomic characterization of K432 and identified a novel plasmid for IMP transmission. Further investigation and surveillance are warranted for pK432-IMP-type plasmid. While routine monitoring of MDR E. hormaechei strains is necessary in China. |
| format | Article |
| id | doaj-art-520a176d722a4ec2b36d1f0efb7abc49 |
| institution | Kabale University |
| issn | 2213-7165 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-520a176d722a4ec2b36d1f0efb7abc492024-12-22T05:28:05ZengElsevierJournal of Global Antimicrobial Resistance2213-71652024-12-0139250256Genomic analysis of IMP-8-producing Enterobacter hormaechei with a novel plasmid pK432-IMPJiao Feng0Zhiwei Song1Piaopiao Dai2Huimin Chen3Dakang Hu4Lianhua Yu5Jin Zhang6Xinhua Luo7Institutes of Biomedical Sciences, Shanxi Provincial Key Laboratory of Medical Molecular Cell Biology, Shanxi University, Taiyuan, ChinaDepartment of Clinical Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, ChinaDepartment of Clinical Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, ChinaDepartment of Clinical Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, ChinaDepartment of Clinical Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, ChinaDepartment of Clinical Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, ChinaDepartment of Clinical Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, ChinaDepartment of Clinical Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, China; Corresponding author. Mailing address: Department of Clinical Laboratory Medicine, Taizhou Municipal Hospital, No. 381-1 Zhongshan East Road, Jiaojiang District, Taizhou 318000, China.ABSTRACT: Background: Carbapenem-resistant Enterobacter cloacae (CR-ECC) complex has posed significant challenges to the clinical treatment of infections, and Enterobacter hormaechei (E. hormaechei) is the most commonly identified nosocomial pathogen of CR-ECC. Methods: Carbapenemases were detected by the immunocolloidal gold technique. The minimum inhibitory concentration (MIC) values were determined by VITEK2. The genome sequence of strain K432 was obtained and analysed. Results: We isolated the IMP-8-producing E. hormaechei strain K432 from a patient's urine specimen in a Chinese hospital, which exhibited resistance to multiple antibiotics, including ceftazidime, piperacillin/tazobactam, aztreonam, imipenem, meropenem, ciprofloxacin, levofloxacin, minocycline, and trimethoprim/sulfamethoxazole. The genome of strain K432 was composed of the chromosome cK432 (4863 kb) and 3 plasmids: pK432-IMP (45.8 kb), pK432-TEM (75.6 kb), and pK432-NR (4.8 kb). In K432, six drug-resistant genes were detected, including blaACT-7 and fosA on cK432, blaIMP-8, and qnrS1 on pK432-IMP, blaSFO-1, and blaTEM-1 on pK432-TEM. The pK432-IMP belonged to a novel incompatibility group, and pK432-TEM was an incompatibility (Inc) R plasmid. Both of these two plasmids shared similar conserved backbone regions with their reference plasmids, respectively. However, the single accessory regions in these two plasmids were different from their reference plasmids, indicating that new recombination and integration events had occurred in K432. Conclusions: This study provides a comprehensive understanding of the genomic characterization of K432 and identified a novel plasmid for IMP transmission. Further investigation and surveillance are warranted for pK432-IMP-type plasmid. While routine monitoring of MDR E. hormaechei strains is necessary in China.http://www.sciencedirect.com/science/article/pii/S2213716524004429Enterobacter hormaecheiblaIMP-8pK432-IMP |
| spellingShingle | Jiao Feng Zhiwei Song Piaopiao Dai Huimin Chen Dakang Hu Lianhua Yu Jin Zhang Xinhua Luo Genomic analysis of IMP-8-producing Enterobacter hormaechei with a novel plasmid pK432-IMP Journal of Global Antimicrobial Resistance Enterobacter hormaechei blaIMP-8 pK432-IMP |
| title | Genomic analysis of IMP-8-producing Enterobacter hormaechei with a novel plasmid pK432-IMP |
| title_full | Genomic analysis of IMP-8-producing Enterobacter hormaechei with a novel plasmid pK432-IMP |
| title_fullStr | Genomic analysis of IMP-8-producing Enterobacter hormaechei with a novel plasmid pK432-IMP |
| title_full_unstemmed | Genomic analysis of IMP-8-producing Enterobacter hormaechei with a novel plasmid pK432-IMP |
| title_short | Genomic analysis of IMP-8-producing Enterobacter hormaechei with a novel plasmid pK432-IMP |
| title_sort | genomic analysis of imp 8 producing enterobacter hormaechei with a novel plasmid pk432 imp |
| topic | Enterobacter hormaechei blaIMP-8 pK432-IMP |
| url | http://www.sciencedirect.com/science/article/pii/S2213716524004429 |
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