PTPRC is an immunotherapeutic predictor and serum biomarker in lung adenocarcinoma correlated with immune cell infiltration
Abstract Lung adenocarcinoma (LUAD) is the most common subtype of lung cancer, with the poor overall prognosis. PTPRC is an essential protein on the surface of cells of the immunological systems However, the role of PTPRC in LUAD has not been clarified. UALCAN, HPA and TCGA database were used to inv...
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Nature Portfolio
2025-08-01
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| Online Access: | https://doi.org/10.1038/s41598-025-15565-w |
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| author | Yongwang Hou Zhichao Yang Zhicong Yang Bin Zhang Jiangmin Liu Dandan Xu Baoli Xiang |
| author_facet | Yongwang Hou Zhichao Yang Zhicong Yang Bin Zhang Jiangmin Liu Dandan Xu Baoli Xiang |
| author_sort | Yongwang Hou |
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| description | Abstract Lung adenocarcinoma (LUAD) is the most common subtype of lung cancer, with the poor overall prognosis. PTPRC is an essential protein on the surface of cells of the immunological systems However, the role of PTPRC in LUAD has not been clarified. UALCAN, HPA and TCGA database were used to investigate PTPRC expression in LUAD. HPA and Kaplan-Meier plotter database were used to explore the survival curve evaluating the prognostic value of PTPRC for LUAD. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis of PTPRC co-expression genes downloaded from cBioPortal database. Tumor immune dysfunction and exclusion (TIDE) was used to predict the immunotherapy response in different PTPRC subgroups. TIMER was used to analyze the correlation of PTPRC and immune cell infiltration or immune checkpoint expression level in LUAD. Serum PTPRC were detected by enzyme linked immunosorbent assay. In present study, the expression of PTPRC in LUAD was lower than that in the normal control group by means of bioinformatics analysis of the TCGA and UALCAN public databases. Moreover, LUAD patients with downregulated PTPRC expression exhibited poor overall survival. Besides, PTPRC was significantly positively correlated with various immune cells in LUAD. TIDE scores were markedly elevated in the PTPRC low-expression subgroup in contrast to the PTPRC high-expression subgroup, And PTPRC was also markedly positively associated with biomarkers of these infiltrated immune cells. PTPRC expression was also positively correlated with PD-1, PD-L1 and CTLA-4 expression. The serum PTPRC levels in LUAD patients were significantly reduced compared to the normal health group. The ROC curve of PTPRC concentration in LUAD was plotted to obtain an AUC = 0.7887, the cutoff value was 31.55 pg/mL, and the sensitivity and specificity were 77.78 and 70.00%, respectively. In summary, our results indicate that down-regulation of PTPRC predicts poor prognosis and associated with immune infiltration in LUAD and PTPRC is an immunotherapeutic predictor and serum biomarker in LUAD correlated with immune cell infiltration. |
| format | Article |
| id | doaj-art-51fe289ff6de45708aa8c4c6ef074152 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-51fe289ff6de45708aa8c4c6ef0741522025-08-20T03:43:21ZengNature PortfolioScientific Reports2045-23222025-08-0115111310.1038/s41598-025-15565-wPTPRC is an immunotherapeutic predictor and serum biomarker in lung adenocarcinoma correlated with immune cell infiltrationYongwang Hou0Zhichao Yang1Zhicong Yang2Bin Zhang3Jiangmin Liu4Dandan Xu5Baoli Xiang6Clinical Laboratory, The First Affiliated Hospital of Hebei North UniversityGraduate School of Hebei North UniversityCentral Laboratory, The First Affiliated Hospital of Hebei North UniversityClinical Laboratory, The First Affiliated Hospital of Hebei North UniversityClinical Laboratory, The First Affiliated Hospital of Hebei North UniversityCentral Laboratory, The First Affiliated Hospital of Hebei North UniversityRespiratory Department, The First Affiliated Hospital of Hebei North UniversityAbstract Lung adenocarcinoma (LUAD) is the most common subtype of lung cancer, with the poor overall prognosis. PTPRC is an essential protein on the surface of cells of the immunological systems However, the role of PTPRC in LUAD has not been clarified. UALCAN, HPA and TCGA database were used to investigate PTPRC expression in LUAD. HPA and Kaplan-Meier plotter database were used to explore the survival curve evaluating the prognostic value of PTPRC for LUAD. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis of PTPRC co-expression genes downloaded from cBioPortal database. Tumor immune dysfunction and exclusion (TIDE) was used to predict the immunotherapy response in different PTPRC subgroups. TIMER was used to analyze the correlation of PTPRC and immune cell infiltration or immune checkpoint expression level in LUAD. Serum PTPRC were detected by enzyme linked immunosorbent assay. In present study, the expression of PTPRC in LUAD was lower than that in the normal control group by means of bioinformatics analysis of the TCGA and UALCAN public databases. Moreover, LUAD patients with downregulated PTPRC expression exhibited poor overall survival. Besides, PTPRC was significantly positively correlated with various immune cells in LUAD. TIDE scores were markedly elevated in the PTPRC low-expression subgroup in contrast to the PTPRC high-expression subgroup, And PTPRC was also markedly positively associated with biomarkers of these infiltrated immune cells. PTPRC expression was also positively correlated with PD-1, PD-L1 and CTLA-4 expression. The serum PTPRC levels in LUAD patients were significantly reduced compared to the normal health group. The ROC curve of PTPRC concentration in LUAD was plotted to obtain an AUC = 0.7887, the cutoff value was 31.55 pg/mL, and the sensitivity and specificity were 77.78 and 70.00%, respectively. In summary, our results indicate that down-regulation of PTPRC predicts poor prognosis and associated with immune infiltration in LUAD and PTPRC is an immunotherapeutic predictor and serum biomarker in LUAD correlated with immune cell infiltration.https://doi.org/10.1038/s41598-025-15565-wLung adenocarcinomaPTPRCImmune infiltrationImmunotherapyBiomarker |
| spellingShingle | Yongwang Hou Zhichao Yang Zhicong Yang Bin Zhang Jiangmin Liu Dandan Xu Baoli Xiang PTPRC is an immunotherapeutic predictor and serum biomarker in lung adenocarcinoma correlated with immune cell infiltration Scientific Reports Lung adenocarcinoma PTPRC Immune infiltration Immunotherapy Biomarker |
| title | PTPRC is an immunotherapeutic predictor and serum biomarker in lung adenocarcinoma correlated with immune cell infiltration |
| title_full | PTPRC is an immunotherapeutic predictor and serum biomarker in lung adenocarcinoma correlated with immune cell infiltration |
| title_fullStr | PTPRC is an immunotherapeutic predictor and serum biomarker in lung adenocarcinoma correlated with immune cell infiltration |
| title_full_unstemmed | PTPRC is an immunotherapeutic predictor and serum biomarker in lung adenocarcinoma correlated with immune cell infiltration |
| title_short | PTPRC is an immunotherapeutic predictor and serum biomarker in lung adenocarcinoma correlated with immune cell infiltration |
| title_sort | ptprc is an immunotherapeutic predictor and serum biomarker in lung adenocarcinoma correlated with immune cell infiltration |
| topic | Lung adenocarcinoma PTPRC Immune infiltration Immunotherapy Biomarker |
| url | https://doi.org/10.1038/s41598-025-15565-w |
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