Influence of Bacteriospermia, Host and Lifestyle Factors on Sperm DNA Integrity: A Cross-Sectional Study Based on a Fertility Center of Nepal
Objective: To determine the sperm DNA fragmentation in the semen of suspected sub-fertile men of Nepal and find its association with bacteriospermia demographic and lifestyle factors. Materials and methods: A cross-sectional study was conducted with the subjects, males among sub-fertile couples v...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2025-04-01
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| Series: | Journal of Family and Reproductive Health |
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| Online Access: | https://jfrh.tums.ac.ir/index.php/jfrh/article/view/2978 |
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| author | Anima Shrestha Dev Raj Joshi Dijan Vaidya Sanu Maiya Shrestha Anjana Singh |
| author_facet | Anima Shrestha Dev Raj Joshi Dijan Vaidya Sanu Maiya Shrestha Anjana Singh |
| author_sort | Anima Shrestha |
| collection | DOAJ |
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Objective: To determine the sperm DNA fragmentation in the semen of suspected sub-fertile men of Nepal and find its association with bacteriospermia demographic and lifestyle factors.
Materials and methods: A cross-sectional study was conducted with the subjects, males among
sub-fertile couples visiting a fertility center in Kathmandu Valley, Nepal for consultation. Information on demography and exposure factors was obtained with a structured questionnaire, and bacteriospermia and sperm DNA fragmentation was determined from the semen samples collected from the study subjects. The data obtained were used to assess sperm DNA fragmentation and its association with various risk factors in sub-fertile men of Nepal.
Results: Out of 186 samples analyzed, 41.4% had low DNA fragmentation (<15%), 38.7% had
moderate DNA fragmentation (≥15% and <30%), and 19.9% had high DNA fragmentation (≥30%). Among the risk factors analyzed, sperm DNA fragmentation was found to be significantly associated with the age of the patients (p<0.05). Other factors analyzed body mass index, smoking, alcohol consumption, physical activity, and bacteriospermia were not found to be associated with sperm DNA fragmentation in our study.
Conclusion: Sperm DNA integrity may be distorted with the increasing age of men, leading to decreasing fertility potential.
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| format | Article |
| id | doaj-art-51fab07f57b84dee9b1ae6a7f4f192f4 |
| institution | OA Journals |
| issn | 1735-8949 1735-9392 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Family and Reproductive Health |
| spelling | doaj-art-51fab07f57b84dee9b1ae6a7f4f192f42025-08-20T02:28:41ZengTehran University of Medical SciencesJournal of Family and Reproductive Health1735-89491735-93922025-04-0119110.18502/jfrh.v19i1.18438Influence of Bacteriospermia, Host and Lifestyle Factors on Sperm DNA Integrity: A Cross-Sectional Study Based on a Fertility Center of NepalAnima Shrestha0Dev Raj Joshi1Dijan Vaidya2Sanu Maiya Shrestha3Anjana Singh4Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal AND Tri-Chandra Multiple Campus, Tribhuvan University, Kathmandu, NepalCentral Department of Microbiology, Tribhuvan University, Kathmandu, NepalCreator’s IVF Nepal Pvt. Ltd., Lalitpur, NepalCreator’s IVF Nepal Pvt. Ltd., Lalitpur, NepalCentral Department of Microbiology, Tribhuvan University, Kathmandu, Nepal Objective: To determine the sperm DNA fragmentation in the semen of suspected sub-fertile men of Nepal and find its association with bacteriospermia demographic and lifestyle factors. Materials and methods: A cross-sectional study was conducted with the subjects, males among sub-fertile couples visiting a fertility center in Kathmandu Valley, Nepal for consultation. Information on demography and exposure factors was obtained with a structured questionnaire, and bacteriospermia and sperm DNA fragmentation was determined from the semen samples collected from the study subjects. The data obtained were used to assess sperm DNA fragmentation and its association with various risk factors in sub-fertile men of Nepal. Results: Out of 186 samples analyzed, 41.4% had low DNA fragmentation (<15%), 38.7% had moderate DNA fragmentation (≥15% and <30%), and 19.9% had high DNA fragmentation (≥30%). Among the risk factors analyzed, sperm DNA fragmentation was found to be significantly associated with the age of the patients (p<0.05). Other factors analyzed body mass index, smoking, alcohol consumption, physical activity, and bacteriospermia were not found to be associated with sperm DNA fragmentation in our study. Conclusion: Sperm DNA integrity may be distorted with the increasing age of men, leading to decreasing fertility potential. https://jfrh.tums.ac.ir/index.php/jfrh/article/view/2978AgeBacteriaMale InfertilitySperm DNA Fragmentation |
| spellingShingle | Anima Shrestha Dev Raj Joshi Dijan Vaidya Sanu Maiya Shrestha Anjana Singh Influence of Bacteriospermia, Host and Lifestyle Factors on Sperm DNA Integrity: A Cross-Sectional Study Based on a Fertility Center of Nepal Journal of Family and Reproductive Health Age Bacteria Male Infertility Sperm DNA Fragmentation |
| title | Influence of Bacteriospermia, Host and Lifestyle Factors on Sperm DNA Integrity: A Cross-Sectional Study Based on a Fertility Center of Nepal |
| title_full | Influence of Bacteriospermia, Host and Lifestyle Factors on Sperm DNA Integrity: A Cross-Sectional Study Based on a Fertility Center of Nepal |
| title_fullStr | Influence of Bacteriospermia, Host and Lifestyle Factors on Sperm DNA Integrity: A Cross-Sectional Study Based on a Fertility Center of Nepal |
| title_full_unstemmed | Influence of Bacteriospermia, Host and Lifestyle Factors on Sperm DNA Integrity: A Cross-Sectional Study Based on a Fertility Center of Nepal |
| title_short | Influence of Bacteriospermia, Host and Lifestyle Factors on Sperm DNA Integrity: A Cross-Sectional Study Based on a Fertility Center of Nepal |
| title_sort | influence of bacteriospermia host and lifestyle factors on sperm dna integrity a cross sectional study based on a fertility center of nepal |
| topic | Age Bacteria Male Infertility Sperm DNA Fragmentation |
| url | https://jfrh.tums.ac.ir/index.php/jfrh/article/view/2978 |
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