Advances in Hepatic Tissue Bioengineering with Decellularized Liver Bioscaffold

The burden of liver diseases continues to grow worldwide, and liver transplantation is the only option for patients with end-stage liver disease. This procedure is limited by critical issues, including the low availability of donor organs; thus, novel therapeutic strategies are greatly needed. Recen...

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Main Authors: Erik Aranha Rossi, Luiz Fernando Quintanilha, Carolina Kymie Vasques Nonaka, Bruno Solano de Freitas Souza
Format: Article
Language:English
Published: Wiley 2019-01-01
Series:Stem Cells International
Online Access:http://dx.doi.org/10.1155/2019/2693189
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author Erik Aranha Rossi
Luiz Fernando Quintanilha
Carolina Kymie Vasques Nonaka
Bruno Solano de Freitas Souza
author_facet Erik Aranha Rossi
Luiz Fernando Quintanilha
Carolina Kymie Vasques Nonaka
Bruno Solano de Freitas Souza
author_sort Erik Aranha Rossi
collection DOAJ
description The burden of liver diseases continues to grow worldwide, and liver transplantation is the only option for patients with end-stage liver disease. This procedure is limited by critical issues, including the low availability of donor organs; thus, novel therapeutic strategies are greatly needed. Recently, bioengineering approaches using decellularized liver scaffolds have been proposed as a novel strategy to overcome these challenges. The aim of this systematic literature review was to identify the major advances in the field of bioengineering using decellularized liver scaffolds and to identify obstacles and challenges for clinical application. The main findings of the articles and each contribution for technique optimization were highlighted, including the protocols of perfusion and decellularization, duration, demonstration of quality control—scaffold acellularity, matrix composition, and preservation of growth factors—and tissue functionality after recellularization. In previous years, many advances have been made as this technique has evolved from studies in animal models to human livers. As the field develops and this promising technique has become much more feasible, many challenges remain, including the selection of appropriate cell types for recellularization, route of cell administration, cell-seeding protocol, and scalability that must be standardized prior to clinical application.
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spelling doaj-art-51f5e95068fe49c49e35917ac668e6ec2025-08-20T03:36:27ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/26931892693189Advances in Hepatic Tissue Bioengineering with Decellularized Liver BioscaffoldErik Aranha Rossi0Luiz Fernando Quintanilha1Carolina Kymie Vasques Nonaka2Bruno Solano de Freitas Souza3Escola de Ciências da Saúde-Universidade Salvador-UNIFACS, Salvador, BA, BrazilEscola de Ciências da Saúde-Universidade Salvador-UNIFACS, Salvador, BA, BrazilSão Rafael Hospital, D’Or Institute for Research and Education (IDOR), Salvador, BA, BrazilSão Rafael Hospital, D’Or Institute for Research and Education (IDOR), Salvador, BA, BrazilThe burden of liver diseases continues to grow worldwide, and liver transplantation is the only option for patients with end-stage liver disease. This procedure is limited by critical issues, including the low availability of donor organs; thus, novel therapeutic strategies are greatly needed. Recently, bioengineering approaches using decellularized liver scaffolds have been proposed as a novel strategy to overcome these challenges. The aim of this systematic literature review was to identify the major advances in the field of bioengineering using decellularized liver scaffolds and to identify obstacles and challenges for clinical application. The main findings of the articles and each contribution for technique optimization were highlighted, including the protocols of perfusion and decellularization, duration, demonstration of quality control—scaffold acellularity, matrix composition, and preservation of growth factors—and tissue functionality after recellularization. In previous years, many advances have been made as this technique has evolved from studies in animal models to human livers. As the field develops and this promising technique has become much more feasible, many challenges remain, including the selection of appropriate cell types for recellularization, route of cell administration, cell-seeding protocol, and scalability that must be standardized prior to clinical application.http://dx.doi.org/10.1155/2019/2693189
spellingShingle Erik Aranha Rossi
Luiz Fernando Quintanilha
Carolina Kymie Vasques Nonaka
Bruno Solano de Freitas Souza
Advances in Hepatic Tissue Bioengineering with Decellularized Liver Bioscaffold
Stem Cells International
title Advances in Hepatic Tissue Bioengineering with Decellularized Liver Bioscaffold
title_full Advances in Hepatic Tissue Bioengineering with Decellularized Liver Bioscaffold
title_fullStr Advances in Hepatic Tissue Bioengineering with Decellularized Liver Bioscaffold
title_full_unstemmed Advances in Hepatic Tissue Bioengineering with Decellularized Liver Bioscaffold
title_short Advances in Hepatic Tissue Bioengineering with Decellularized Liver Bioscaffold
title_sort advances in hepatic tissue bioengineering with decellularized liver bioscaffold
url http://dx.doi.org/10.1155/2019/2693189
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AT luizfernandoquintanilha advancesinhepatictissuebioengineeringwithdecellularizedliverbioscaffold
AT carolinakymievasquesnonaka advancesinhepatictissuebioengineeringwithdecellularizedliverbioscaffold
AT brunosolanodefreitassouza advancesinhepatictissuebioengineeringwithdecellularizedliverbioscaffold