Extraction, Purification and Immune Activity of Polysaccharide from Naxi Wuniu Tea
Objective: The aim of this study was to isolate and purify the polysaccharide from Naxi Wuniu special morning tea, and the antioxidant and immune activities were further investigated. Methods: Firstly, the tea polysaccharides were extracted by hot water extraction method, and further purified by DEA...
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The editorial department of Science and Technology of Food Industry
2025-03-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030456 |
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| author | Ling LAI Xiao YANG Lin YIN Xiaoyu CHEN Huirong YANG Qumin PENG Jun LIU Yingjie ZENG |
| author_facet | Ling LAI Xiao YANG Lin YIN Xiaoyu CHEN Huirong YANG Qumin PENG Jun LIU Yingjie ZENG |
| author_sort | Ling LAI |
| collection | DOAJ |
| description | Objective: The aim of this study was to isolate and purify the polysaccharide from Naxi Wuniu special morning tea, and the antioxidant and immune activities were further investigated. Methods: Firstly, the tea polysaccharides were extracted by hot water extraction method, and further purified by DEAE-52 and dextranan gel G200. Then, the basic physicochemical properties of polysaccharide were studied. And the antioxidant activities were evaluated by determined the 1,1 Diphenyl-2-trinitrobenzene hydrazine (DPPH) free radical scavenging capacity, 2,2' -diazo-di-3-ethylbenzothiazolin-6-sulfonic acid (ABTS) cationic free radical scavenging capacity, and oxygen radical absorbance capacity (ORAC). Then, the safeties of tea polysaccharide were analyzed, including endotoxin detection, RAW264.7 cells and human embryonic kidney cell 293 (HEK293) toxicity detection. Finally, the effects of tea polysaccharide on RAW264.7 cytokines (interleukin-6, IL6, tumor necrosis factor α, TNF-α, NO) release and the corresponding mRNA secretion levels, in order to obtain the immune-enhancing activity of tea polysaccharide. Results: A purified polysaccharide fraction WNp was obtained from Naxi Wuniu morning tea by using DEAE-52 anion exchange resin and Sephadex G-200. Physicochemical analysis showed that the purity of WNp was 90.26%, the protein content of WNp was 1.37%, and WNp did not contain triple helix structure. WNp was composed of glucose (Glc), galactose (Gal), arabinose (Ara), mannose (Man), rhamnose (Rha), glucuronic acid (GlcA) and galacturonic acid (GalA) at the mole ratio of 0.4:32.6:2.3:4.7:8.7:0.3:40.3, respectively. The molecular weight of WNp was 82.61 kDa. WNp showed significant scavenging abilities on DPPH and ABTS+ free radicals and a high ORAC value, that was, it had good antioxidant activities. Finally, WNp could up-regulate the mRNA expression of cytokines (IL-6, TNF-α) and NO, thereby promoting cytokine secretion (IL-6, TNF-α) and NO release, and then activate the initial immune response of RAW264.7 cells, which achieved the immuno-enhancing activity. Conclusion: The research results provide scientific basis for the deep processing and utilization of Naxi Wulu tea source. |
| format | Article |
| id | doaj-art-51ee3e1164744a0facb6fa4b5d1650e9 |
| institution | DOAJ |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-03-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| spelling | doaj-art-51ee3e1164744a0facb6fa4b5d1650e92025-08-20T02:58:44ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-03-0146623124110.13386/j.issn1002-0306.20240304562024030456-6Extraction, Purification and Immune Activity of Polysaccharide from Naxi Wuniu TeaLing LAI0Xiao YANG1Lin YIN2Xiaoyu CHEN3Huirong YANG4Qumin PENG5Jun LIU6Yingjie ZENG7College of Food Science and Technology, Xinan Minzu Univercity, Chengdu 610041, ChinaCollege of Food Science and Technology, Xinan Minzu Univercity, Chengdu 610041, ChinaCollege of Food Science and Technology, Xinan Minzu Univercity, Chengdu 610041, ChinaCollege of Food Science and Technology, Xinan Minzu Univercity, Chengdu 610041, ChinaCollege of Food Science and Technology, Xinan Minzu Univercity, Chengdu 610041, ChinaDadukou Town, Naxi District, Luzhou 646329, ChinaSichuan Dalit Morning Tea Co., Ltd., Luzhou 646318, ChinaCollege of Food Science and Technology, Xinan Minzu Univercity, Chengdu 610041, ChinaObjective: The aim of this study was to isolate and purify the polysaccharide from Naxi Wuniu special morning tea, and the antioxidant and immune activities were further investigated. Methods: Firstly, the tea polysaccharides were extracted by hot water extraction method, and further purified by DEAE-52 and dextranan gel G200. Then, the basic physicochemical properties of polysaccharide were studied. And the antioxidant activities were evaluated by determined the 1,1 Diphenyl-2-trinitrobenzene hydrazine (DPPH) free radical scavenging capacity, 2,2' -diazo-di-3-ethylbenzothiazolin-6-sulfonic acid (ABTS) cationic free radical scavenging capacity, and oxygen radical absorbance capacity (ORAC). Then, the safeties of tea polysaccharide were analyzed, including endotoxin detection, RAW264.7 cells and human embryonic kidney cell 293 (HEK293) toxicity detection. Finally, the effects of tea polysaccharide on RAW264.7 cytokines (interleukin-6, IL6, tumor necrosis factor α, TNF-α, NO) release and the corresponding mRNA secretion levels, in order to obtain the immune-enhancing activity of tea polysaccharide. Results: A purified polysaccharide fraction WNp was obtained from Naxi Wuniu morning tea by using DEAE-52 anion exchange resin and Sephadex G-200. Physicochemical analysis showed that the purity of WNp was 90.26%, the protein content of WNp was 1.37%, and WNp did not contain triple helix structure. WNp was composed of glucose (Glc), galactose (Gal), arabinose (Ara), mannose (Man), rhamnose (Rha), glucuronic acid (GlcA) and galacturonic acid (GalA) at the mole ratio of 0.4:32.6:2.3:4.7:8.7:0.3:40.3, respectively. The molecular weight of WNp was 82.61 kDa. WNp showed significant scavenging abilities on DPPH and ABTS+ free radicals and a high ORAC value, that was, it had good antioxidant activities. Finally, WNp could up-regulate the mRNA expression of cytokines (IL-6, TNF-α) and NO, thereby promoting cytokine secretion (IL-6, TNF-α) and NO release, and then activate the initial immune response of RAW264.7 cells, which achieved the immuno-enhancing activity. Conclusion: The research results provide scientific basis for the deep processing and utilization of Naxi Wulu tea source.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030456naxi wuniu special morning teapolysaccharidesstructureantioxidant activityimmuno-enhancing activity |
| spellingShingle | Ling LAI Xiao YANG Lin YIN Xiaoyu CHEN Huirong YANG Qumin PENG Jun LIU Yingjie ZENG Extraction, Purification and Immune Activity of Polysaccharide from Naxi Wuniu Tea Shipin gongye ke-ji naxi wuniu special morning tea polysaccharides structure antioxidant activity immuno-enhancing activity |
| title | Extraction, Purification and Immune Activity of Polysaccharide from Naxi Wuniu Tea |
| title_full | Extraction, Purification and Immune Activity of Polysaccharide from Naxi Wuniu Tea |
| title_fullStr | Extraction, Purification and Immune Activity of Polysaccharide from Naxi Wuniu Tea |
| title_full_unstemmed | Extraction, Purification and Immune Activity of Polysaccharide from Naxi Wuniu Tea |
| title_short | Extraction, Purification and Immune Activity of Polysaccharide from Naxi Wuniu Tea |
| title_sort | extraction purification and immune activity of polysaccharide from naxi wuniu tea |
| topic | naxi wuniu special morning tea polysaccharides structure antioxidant activity immuno-enhancing activity |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030456 |
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