Vapor pressure deficit control and mechanical vibration techniques to induce self-pollination in strawberry flowers

Abstract Background Pollination strategies to supplement or replace insect pollinators are needed to produce marketable strawberry fruits in indoor vertical farms. To ensure the self-pollination of strawberry flowers, anther dehiscence, and pollen attachment were investigated under different vapor p...

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Main Authors: Hyein Lee, Meiyan Cui, Byungkwan Lee, Jeesang Myung, Jaewook Shin, Changhoo Chun
Format: Article
Language:English
Published: BMC 2025-02-01
Series:Plant Methods
Subjects:
Online Access:https://doi.org/10.1186/s13007-025-01343-2
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author Hyein Lee
Meiyan Cui
Byungkwan Lee
Jeesang Myung
Jaewook Shin
Changhoo Chun
author_facet Hyein Lee
Meiyan Cui
Byungkwan Lee
Jeesang Myung
Jaewook Shin
Changhoo Chun
author_sort Hyein Lee
collection DOAJ
description Abstract Background Pollination strategies to supplement or replace insect pollinators are needed to produce marketable strawberry fruits in indoor vertical farms. To ensure the self-pollination of strawberry flowers, anther dehiscence, and pollen attachment were investigated under different vapor pressure deficit (VPD) conditions and external mechanical wave vibrations. Results The proportion of dehisced anthers was examined under VPDs of 2.06, 1.58, and 0.33 kPa, and the projected area of pollen clumps was assessed under VPDs of 2.06 and 0.33 kPa. After exposing flowers to a VPD of 2.06 kPa, vibrations with various frequency (Hz) and root mean square acceleration (m s−2) combinations were used to evaluate pollination effectiveness. The anthers underwent complete dehiscence at VPDs of 2.06, 1.58, and 0.33 kPa. The pollen clump ejection index was highest at a VPD of 2.06 kPa. Pollen clump detachment was effective at 800 Hz with 40 m s−2, while pollen attachment to the stigma was most effective at 100 Hz with 30 and 40 m s−2. Conclusions These findings demonstrate that high VPD promotes anther dehiscence timing and facilitates pollen clump formation, while specific vibration frequencies with high acceleration optimize pollen detachment and stigma attachment, offering an effective strategy for controlled strawberry pollination in vertical farming.
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spelling doaj-art-51ee2edfe60b41b280a2901ef84a7d072025-08-20T02:16:55ZengBMCPlant Methods1746-48112025-02-0121111310.1186/s13007-025-01343-2Vapor pressure deficit control and mechanical vibration techniques to induce self-pollination in strawberry flowersHyein Lee0Meiyan Cui1Byungkwan Lee2Jeesang Myung3Jaewook Shin4Changhoo Chun5Department of Agriculture, Forestry and Bioresources, Seoul National UniversityResearch Institute of Agriculture and Life Sciences, Seoul National UniversityDepartment of Agriculture, Forestry and Bioresources, Seoul National UniversityDepartment of Agriculture, Forestry and Bioresources, Seoul National UniversityDepartment of Agriculture, Forestry and Bioresources, Seoul National UniversityDepartment of Agriculture, Forestry and Bioresources, Seoul National UniversityAbstract Background Pollination strategies to supplement or replace insect pollinators are needed to produce marketable strawberry fruits in indoor vertical farms. To ensure the self-pollination of strawberry flowers, anther dehiscence, and pollen attachment were investigated under different vapor pressure deficit (VPD) conditions and external mechanical wave vibrations. Results The proportion of dehisced anthers was examined under VPDs of 2.06, 1.58, and 0.33 kPa, and the projected area of pollen clumps was assessed under VPDs of 2.06 and 0.33 kPa. After exposing flowers to a VPD of 2.06 kPa, vibrations with various frequency (Hz) and root mean square acceleration (m s−2) combinations were used to evaluate pollination effectiveness. The anthers underwent complete dehiscence at VPDs of 2.06, 1.58, and 0.33 kPa. The pollen clump ejection index was highest at a VPD of 2.06 kPa. Pollen clump detachment was effective at 800 Hz with 40 m s−2, while pollen attachment to the stigma was most effective at 100 Hz with 30 and 40 m s−2. Conclusions These findings demonstrate that high VPD promotes anther dehiscence timing and facilitates pollen clump formation, while specific vibration frequencies with high acceleration optimize pollen detachment and stigma attachment, offering an effective strategy for controlled strawberry pollination in vertical farming.https://doi.org/10.1186/s13007-025-01343-2Pollination strategySelf-pollinationStrawberry flowerVibration pollinationVPD conditions
spellingShingle Hyein Lee
Meiyan Cui
Byungkwan Lee
Jeesang Myung
Jaewook Shin
Changhoo Chun
Vapor pressure deficit control and mechanical vibration techniques to induce self-pollination in strawberry flowers
Plant Methods
Pollination strategy
Self-pollination
Strawberry flower
Vibration pollination
VPD conditions
title Vapor pressure deficit control and mechanical vibration techniques to induce self-pollination in strawberry flowers
title_full Vapor pressure deficit control and mechanical vibration techniques to induce self-pollination in strawberry flowers
title_fullStr Vapor pressure deficit control and mechanical vibration techniques to induce self-pollination in strawberry flowers
title_full_unstemmed Vapor pressure deficit control and mechanical vibration techniques to induce self-pollination in strawberry flowers
title_short Vapor pressure deficit control and mechanical vibration techniques to induce self-pollination in strawberry flowers
title_sort vapor pressure deficit control and mechanical vibration techniques to induce self pollination in strawberry flowers
topic Pollination strategy
Self-pollination
Strawberry flower
Vibration pollination
VPD conditions
url https://doi.org/10.1186/s13007-025-01343-2
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