Sealless Production Ultracentrifuge and Its Magnetically Self-Bearing Openable Motors for Purification in Viral Nanotechnology
Viral nanotechnology enables new possibilities for gene therapies and vaccines. However, the manufacturing of viral vectors lacks a satisfactory production capable method for purification of full capsids. Empty or partially empty capsids need to be removed to avoid immunotoxicity. The state of the a...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
IEEE
2025-01-01
|
| Series: | IEEE Access |
| Subjects: | |
| Online Access: | https://ieeexplore.ieee.org/document/10858738/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1825207016961867776 |
|---|---|
| author | Emanuel J. Hubmann Daniel Steinert Thomas Nussbaumer Johann W. Kolar |
| author_facet | Emanuel J. Hubmann Daniel Steinert Thomas Nussbaumer Johann W. Kolar |
| author_sort | Emanuel J. Hubmann |
| collection | DOAJ |
| description | Viral nanotechnology enables new possibilities for gene therapies and vaccines. However, the manufacturing of viral vectors lacks a satisfactory production capable method for purification of full capsids. Empty or partially empty capsids need to be removed to avoid immunotoxicity. The state of the art production ultracentrifuges (PUCFs) are limited, especially in rotational speed to approximately 40 krpm by rotary seals, by complexity and virus containment. This prevents PUCFs from widespread use in industry for viral vector production. This article proposes a novel PUCF type with rotational speed potential towards 100 krpm with a hermetically enclosed process chamber without any rotary seals, i.e. a sealless production ultracentrifuge (SL-PUCF) omitting contamination risk. The openable vertical axis self-bearing motors, the novel sealless flow path design and the needed ultra high-speed potential towards 100 krpm pose new technical problems, for which this article proposes and experimentally validates solutions. A SL-PUCF prototype with its vertical axis magnetically self-bearing openable motors (O-SBMs) and openable burst armor (O-BA) is realized, validated and operated as a system. The general working principle of the SL-PUCF system is experimentally validated by whey protein sedimentation. Furthermore, new future smart capability potential of the proposed new SL-PUCF technology suspended and driven by O-SBMs is unveiled as an outlook and examples shown in case studies. |
| format | Article |
| id | doaj-art-51e8bb76dab841368e2d4206d7825819 |
| institution | Kabale University |
| issn | 2169-3536 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | IEEE |
| record_format | Article |
| series | IEEE Access |
| spelling | doaj-art-51e8bb76dab841368e2d4206d78258192025-02-07T00:01:32ZengIEEEIEEE Access2169-35362025-01-0113230722309010.1109/ACCESS.2025.353755910858738Sealless Production Ultracentrifuge and Its Magnetically Self-Bearing Openable Motors for Purification in Viral NanotechnologyEmanuel J. Hubmann0https://orcid.org/0000-0002-8828-6853Daniel Steinert1https://orcid.org/0000-0002-3400-0653Thomas Nussbaumer2https://orcid.org/0009-0008-0315-4124Johann W. Kolar3https://orcid.org/0000-0002-6000-7402Power Electronic Systems Laboratory, ETH Zürich, Zürich, SwitzerlandLevitronix GmbH, Zürich, SwitzerlandLevitronix GmbH, Zürich, SwitzerlandPower Electronic Systems Laboratory, ETH Zürich, Zürich, SwitzerlandViral nanotechnology enables new possibilities for gene therapies and vaccines. However, the manufacturing of viral vectors lacks a satisfactory production capable method for purification of full capsids. Empty or partially empty capsids need to be removed to avoid immunotoxicity. The state of the art production ultracentrifuges (PUCFs) are limited, especially in rotational speed to approximately 40 krpm by rotary seals, by complexity and virus containment. This prevents PUCFs from widespread use in industry for viral vector production. This article proposes a novel PUCF type with rotational speed potential towards 100 krpm with a hermetically enclosed process chamber without any rotary seals, i.e. a sealless production ultracentrifuge (SL-PUCF) omitting contamination risk. The openable vertical axis self-bearing motors, the novel sealless flow path design and the needed ultra high-speed potential towards 100 krpm pose new technical problems, for which this article proposes and experimentally validates solutions. A SL-PUCF prototype with its vertical axis magnetically self-bearing openable motors (O-SBMs) and openable burst armor (O-BA) is realized, validated and operated as a system. The general working principle of the SL-PUCF system is experimentally validated by whey protein sedimentation. Furthermore, new future smart capability potential of the proposed new SL-PUCF technology suspended and driven by O-SBMs is unveiled as an outlook and examples shown in case studies.https://ieeexplore.ieee.org/document/10858738/Self-bearing motorsmagnetic bearingsopenable motorshigh-speed motorsthermal managementmechatronic system |
| spellingShingle | Emanuel J. Hubmann Daniel Steinert Thomas Nussbaumer Johann W. Kolar Sealless Production Ultracentrifuge and Its Magnetically Self-Bearing Openable Motors for Purification in Viral Nanotechnology IEEE Access Self-bearing motors magnetic bearings openable motors high-speed motors thermal management mechatronic system |
| title | Sealless Production Ultracentrifuge and Its Magnetically Self-Bearing Openable Motors for Purification in Viral Nanotechnology |
| title_full | Sealless Production Ultracentrifuge and Its Magnetically Self-Bearing Openable Motors for Purification in Viral Nanotechnology |
| title_fullStr | Sealless Production Ultracentrifuge and Its Magnetically Self-Bearing Openable Motors for Purification in Viral Nanotechnology |
| title_full_unstemmed | Sealless Production Ultracentrifuge and Its Magnetically Self-Bearing Openable Motors for Purification in Viral Nanotechnology |
| title_short | Sealless Production Ultracentrifuge and Its Magnetically Self-Bearing Openable Motors for Purification in Viral Nanotechnology |
| title_sort | sealless production ultracentrifuge and its magnetically self bearing openable motors for purification in viral nanotechnology |
| topic | Self-bearing motors magnetic bearings openable motors high-speed motors thermal management mechatronic system |
| url | https://ieeexplore.ieee.org/document/10858738/ |
| work_keys_str_mv | AT emanueljhubmann seallessproductionultracentrifugeanditsmagneticallyselfbearingopenablemotorsforpurificationinviralnanotechnology AT danielsteinert seallessproductionultracentrifugeanditsmagneticallyselfbearingopenablemotorsforpurificationinviralnanotechnology AT thomasnussbaumer seallessproductionultracentrifugeanditsmagneticallyselfbearingopenablemotorsforpurificationinviralnanotechnology AT johannwkolar seallessproductionultracentrifugeanditsmagneticallyselfbearingopenablemotorsforpurificationinviralnanotechnology |