The First Molecular Detection of <i>Theileria luwenshuni</i> from <i>Haemaphysalis mageshimaensis</i> on Orchid Island, Taiwan, with No Evidence of SFTSV
Theileriosis is considered an economically important disease that may decrease productivity and cause a high mortality rate in livestock. Only a few studies have reported <i>Theileria</i> spp., such as <i>T. sergenti</i> and <i>T. buffeli</i>, in recent decades in...
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MDPI AG
2025-03-01
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| author | Pai-Shan Chiang I-Jung Tsai Yuan-Wei Hu Hung-Jui Chen I-Jen Chen Hwa-Jen Teng Shiu-Ling Chen |
| author_facet | Pai-Shan Chiang I-Jung Tsai Yuan-Wei Hu Hung-Jui Chen I-Jen Chen Hwa-Jen Teng Shiu-Ling Chen |
| author_sort | Pai-Shan Chiang |
| collection | DOAJ |
| description | Theileriosis is considered an economically important disease that may decrease productivity and cause a high mortality rate in livestock. Only a few studies have reported <i>Theileria</i> spp., such as <i>T. sergenti</i> and <i>T. buffeli</i>, in recent decades in Taiwan. In the present study, 401 ticks have been collected on Orchid Island in June 2022 and April 2023. Our environmental investigation for SFTSV unintentionally discovered <i>T. luwenshuni</i> in <i>Haemaphysalis mageshimaensis</i> on Orchid Island via PCR. The PCR products were sequenced, and the detected 18S rRNA gene sequences shared a 99.65–99.93% identity with <i>T. luwenshuni</i> sequences from ticks and ruminants in Myanmar and China. Despite the difficulty in clarifying the source of <i>T. luwenshuni</i> within neighboring regions, our findings provide an updated distribution of <i>T. luwenshuni</i> in Asia. This is not only the first time that <i>T. luwenshuni</i> was found in <i>H. mageshimaensis</i> but also the first report of <i>T. luwenshuni</i> on Orchid Island, Taiwan. Our study indicates that ruminants may be at risk of infection. Therefore, further investigations are needed to determine the distribution of <i>T. luwenshuni</i> among ruminants on Orchid Island and in Taiwan. |
| format | Article |
| id | doaj-art-51d3521a01e341978981aacd10e2e6d2 |
| institution | Kabale University |
| issn | 2076-0817 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj-art-51d3521a01e341978981aacd10e2e6d22025-08-20T03:43:34ZengMDPI AGPathogens2076-08172025-03-0114324110.3390/pathogens14030241The First Molecular Detection of <i>Theileria luwenshuni</i> from <i>Haemaphysalis mageshimaensis</i> on Orchid Island, Taiwan, with No Evidence of SFTSVPai-Shan Chiang0I-Jung Tsai1Yuan-Wei Hu2Hung-Jui Chen3I-Jen Chen4Hwa-Jen Teng5Shiu-Ling Chen6Center for Diagnostics and Vaccine Development, Centers for Disease Control, Ministry of Health and Welfare, No. 6, Linsen S. Road, Taipei City 10050, TaiwanCenter for Diagnostics and Vaccine Development, Centers for Disease Control, Ministry of Health and Welfare, No. 6, Linsen S. Road, Taipei City 10050, TaiwanTaiwan Forestry Research Institute, No. 53, Nanhai Road, Taipei City 10066, TaiwanCenter for Diagnostics and Vaccine Development, Centers for Disease Control, Ministry of Health and Welfare, No. 6, Linsen S. Road, Taipei City 10050, TaiwanCenter for Diagnostics and Vaccine Development, Centers for Disease Control, Ministry of Health and Welfare, No. 6, Linsen S. Road, Taipei City 10050, TaiwanCenter for Diagnostics and Vaccine Development, Centers for Disease Control, Ministry of Health and Welfare, No. 6, Linsen S. Road, Taipei City 10050, TaiwanCenter for Diagnostics and Vaccine Development, Centers for Disease Control, Ministry of Health and Welfare, No. 6, Linsen S. Road, Taipei City 10050, TaiwanTheileriosis is considered an economically important disease that may decrease productivity and cause a high mortality rate in livestock. Only a few studies have reported <i>Theileria</i> spp., such as <i>T. sergenti</i> and <i>T. buffeli</i>, in recent decades in Taiwan. In the present study, 401 ticks have been collected on Orchid Island in June 2022 and April 2023. Our environmental investigation for SFTSV unintentionally discovered <i>T. luwenshuni</i> in <i>Haemaphysalis mageshimaensis</i> on Orchid Island via PCR. The PCR products were sequenced, and the detected 18S rRNA gene sequences shared a 99.65–99.93% identity with <i>T. luwenshuni</i> sequences from ticks and ruminants in Myanmar and China. Despite the difficulty in clarifying the source of <i>T. luwenshuni</i> within neighboring regions, our findings provide an updated distribution of <i>T. luwenshuni</i> in Asia. This is not only the first time that <i>T. luwenshuni</i> was found in <i>H. mageshimaensis</i> but also the first report of <i>T. luwenshuni</i> on Orchid Island, Taiwan. Our study indicates that ruminants may be at risk of infection. Therefore, further investigations are needed to determine the distribution of <i>T. luwenshuni</i> among ruminants on Orchid Island and in Taiwan.https://www.mdpi.com/2076-0817/14/3/241<i>Theileria luwenshuni</i><i>Haemaphysalis mageshimaensis</i>tickOrchid IslandTaiwan |
| spellingShingle | Pai-Shan Chiang I-Jung Tsai Yuan-Wei Hu Hung-Jui Chen I-Jen Chen Hwa-Jen Teng Shiu-Ling Chen The First Molecular Detection of <i>Theileria luwenshuni</i> from <i>Haemaphysalis mageshimaensis</i> on Orchid Island, Taiwan, with No Evidence of SFTSV Pathogens <i>Theileria luwenshuni</i> <i>Haemaphysalis mageshimaensis</i> tick Orchid Island Taiwan |
| title | The First Molecular Detection of <i>Theileria luwenshuni</i> from <i>Haemaphysalis mageshimaensis</i> on Orchid Island, Taiwan, with No Evidence of SFTSV |
| title_full | The First Molecular Detection of <i>Theileria luwenshuni</i> from <i>Haemaphysalis mageshimaensis</i> on Orchid Island, Taiwan, with No Evidence of SFTSV |
| title_fullStr | The First Molecular Detection of <i>Theileria luwenshuni</i> from <i>Haemaphysalis mageshimaensis</i> on Orchid Island, Taiwan, with No Evidence of SFTSV |
| title_full_unstemmed | The First Molecular Detection of <i>Theileria luwenshuni</i> from <i>Haemaphysalis mageshimaensis</i> on Orchid Island, Taiwan, with No Evidence of SFTSV |
| title_short | The First Molecular Detection of <i>Theileria luwenshuni</i> from <i>Haemaphysalis mageshimaensis</i> on Orchid Island, Taiwan, with No Evidence of SFTSV |
| title_sort | first molecular detection of i theileria luwenshuni i from i haemaphysalis mageshimaensis i on orchid island taiwan with no evidence of sftsv |
| topic | <i>Theileria luwenshuni</i> <i>Haemaphysalis mageshimaensis</i> tick Orchid Island Taiwan |
| url | https://www.mdpi.com/2076-0817/14/3/241 |
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