Corneal Epithelial Removal with a Newly Designed Epithelial Brush
This study aimed to evaluate and compare the effectiveness of a newly developed epithelial removal brush with conventional methods in a rabbit model of corneal epithelial defects. The corneal epithelia of thirty-seven rabbits were removed by three different methods including blades (blade group), ne...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2021/4668056 |
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| author | Ho Seok Chung Seung Hwan Moon Soon-Suk Kang Minseop Kim Hun Lee Hungwon Tchah Jae Yong Kim |
| author_facet | Ho Seok Chung Seung Hwan Moon Soon-Suk Kang Minseop Kim Hun Lee Hungwon Tchah Jae Yong Kim |
| author_sort | Ho Seok Chung |
| collection | DOAJ |
| description | This study aimed to evaluate and compare the effectiveness of a newly developed epithelial removal brush with conventional methods in a rabbit model of corneal epithelial defects. The corneal epithelia of thirty-seven rabbits were removed by three different methods including blades (blade group), newly developed epithelial brushes (Ocu group), and conventional rotating brushes (Amo group). The defect area was measured with light microscopy immediately and at 4, 18, 24, and 50 hours after removal. Corneas were obtained immediately and at 24 and 50 hours and subjected to hematoxylin and eosin (H&E) and immunofluorescence staining using proliferating cell nuclear antigen (PCNA) and phosphorylated heat shock protein 27 (pHSP27) antibodies. The residual stromal surface was observed by scanning electron microscopy (SEM). In the Ocu group, epithelia were significantly recovered at 18, 24, and 50 hours compared with immediately after removal, and in the blade and Amo groups, epithelia were significantly recovered only at 50 hours after epithelial removal. The expression levels of PCNA and pHSP27 did not differ among three groups. There was significantly more inflammatory cell infiltration in the blade group than in the other groups. SEM showed a more regular and uniform residual stromal surface in the Ocu group than in the other groups. The newly developed epithelial brush showed better polishing ability and led to earlier significant epithelial recovery and a more regular and uniform stromal surface than conventional methods in this rabbit model of epithelial defects. Accumulation of clinical data is expected to expand the scope of application of new brushes for laser surface ablation. |
| format | Article |
| id | doaj-art-51ae0954cfc74803a4144fda336b93ce |
| institution | Kabale University |
| issn | 2090-0058 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-51ae0954cfc74803a4144fda336b93ce2025-02-03T01:07:10ZengWileyJournal of Ophthalmology2090-00582021-01-01202110.1155/2021/4668056Corneal Epithelial Removal with a Newly Designed Epithelial BrushHo Seok Chung0Seung Hwan Moon1Soon-Suk Kang2Minseop Kim3Hun Lee4Hungwon Tchah5Jae Yong Kim6Department of OphthalmologyDepartment of OphthalmologyDepartment of OphthalmologyCheonan Seoul Eye CenterDepartment of OphthalmologyDepartment of OphthalmologyDepartment of OphthalmologyThis study aimed to evaluate and compare the effectiveness of a newly developed epithelial removal brush with conventional methods in a rabbit model of corneal epithelial defects. The corneal epithelia of thirty-seven rabbits were removed by three different methods including blades (blade group), newly developed epithelial brushes (Ocu group), and conventional rotating brushes (Amo group). The defect area was measured with light microscopy immediately and at 4, 18, 24, and 50 hours after removal. Corneas were obtained immediately and at 24 and 50 hours and subjected to hematoxylin and eosin (H&E) and immunofluorescence staining using proliferating cell nuclear antigen (PCNA) and phosphorylated heat shock protein 27 (pHSP27) antibodies. The residual stromal surface was observed by scanning electron microscopy (SEM). In the Ocu group, epithelia were significantly recovered at 18, 24, and 50 hours compared with immediately after removal, and in the blade and Amo groups, epithelia were significantly recovered only at 50 hours after epithelial removal. The expression levels of PCNA and pHSP27 did not differ among three groups. There was significantly more inflammatory cell infiltration in the blade group than in the other groups. SEM showed a more regular and uniform residual stromal surface in the Ocu group than in the other groups. The newly developed epithelial brush showed better polishing ability and led to earlier significant epithelial recovery and a more regular and uniform stromal surface than conventional methods in this rabbit model of epithelial defects. Accumulation of clinical data is expected to expand the scope of application of new brushes for laser surface ablation.http://dx.doi.org/10.1155/2021/4668056 |
| spellingShingle | Ho Seok Chung Seung Hwan Moon Soon-Suk Kang Minseop Kim Hun Lee Hungwon Tchah Jae Yong Kim Corneal Epithelial Removal with a Newly Designed Epithelial Brush Journal of Ophthalmology |
| title | Corneal Epithelial Removal with a Newly Designed Epithelial Brush |
| title_full | Corneal Epithelial Removal with a Newly Designed Epithelial Brush |
| title_fullStr | Corneal Epithelial Removal with a Newly Designed Epithelial Brush |
| title_full_unstemmed | Corneal Epithelial Removal with a Newly Designed Epithelial Brush |
| title_short | Corneal Epithelial Removal with a Newly Designed Epithelial Brush |
| title_sort | corneal epithelial removal with a newly designed epithelial brush |
| url | http://dx.doi.org/10.1155/2021/4668056 |
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