Silicon might mitigate nickel toxicity in maize roots via chelation, detoxification, and membrane transport
Nickel is an essential micronutrient for plant growth and development. However, in excessive amounts caused by accidental pollution of soils, this heavy metal is toxic to plants. Although silicon is a non-essential nutrient, it accumulates in most monocots, particularly the vital crop maize (corn, Z...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324014106 |
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| author | Olha Lakhneko Ivana Fialová Roderik Fiala Mária Kopáčová Andrej Kováč Maksym Danchenko |
| author_facet | Olha Lakhneko Ivana Fialová Roderik Fiala Mária Kopáčová Andrej Kováč Maksym Danchenko |
| author_sort | Olha Lakhneko |
| collection | DOAJ |
| description | Nickel is an essential micronutrient for plant growth and development. However, in excessive amounts caused by accidental pollution of soils, this heavy metal is toxic to plants. Although silicon is a non-essential nutrient, it accumulates in most monocots, particularly the vital crop maize (corn, Zea mays). In fact, this metalloid mineral can alleviate the toxicity of heavy metals, though the mechanism is not entirely clear yet. Herein, we measured proteome, gene expression, enzyme activities, and selected sugars to investigate such effect thoroughly. Deep proteomic analysis revealed a minor impact of 100 µM Ni, 2.5 mM Si, or their combination on roots in 12-day-old hydroponically grown maize seedlings upon 9 days of exposure. Nonetheless, we suggested plausible mechanisms of Si mitigation of excessive Ni: Chelation by metallothioneins and phytochelatins, detoxification by glycine betaine pathway, and restructuring of plasma membrane transporters. Higher activity of glutathione S-transferase confirmed its plausible involvement in reducing Ni toxicity in combined treatment. Accumulation of sucrose synthase and corresponding soluble sugars in Ni and combined treatment implied high energy requirements both during heavy metal stress and its mitigation. Expression analysis of genes coding a few differentially accumulated proteins failed to reveal concordant changes, indicating posttranscriptional regulation. Proposed mitigation mechanisms should be functionally validated in follow-up studies. |
| format | Article |
| id | doaj-art-519a1af101c445318a9c18aaa98a4cdb |
| institution | OA Journals |
| issn | 0147-6513 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-519a1af101c445318a9c18aaa98a4cdb2025-08-20T02:37:32ZengElsevierEcotoxicology and Environmental Safety0147-65132024-12-0128811733410.1016/j.ecoenv.2024.117334Silicon might mitigate nickel toxicity in maize roots via chelation, detoxification, and membrane transportOlha Lakhneko0Ivana Fialová1Roderik Fiala2Mária Kopáčová3Andrej Kováč4Maksym Danchenko5Plant Science and Biodiversity Centre, Slovak Academy of Sciences, Bratislava 84523, SlovakiaPlant Science and Biodiversity Centre, Slovak Academy of Sciences, Bratislava 84523, SlovakiaPlant Science and Biodiversity Centre, Slovak Academy of Sciences, Bratislava 84523, SlovakiaInstitute of Chemistry, Slovak Academy of Sciences, Bratislava 84538, SlovakiaInstitute of Neuroimmunology, Slovak Academy of Sciences, Bratislava 84510, SlovakiaPlant Science and Biodiversity Centre, Slovak Academy of Sciences, Bratislava 84523, Slovakia; Correspondence author.Nickel is an essential micronutrient for plant growth and development. However, in excessive amounts caused by accidental pollution of soils, this heavy metal is toxic to plants. Although silicon is a non-essential nutrient, it accumulates in most monocots, particularly the vital crop maize (corn, Zea mays). In fact, this metalloid mineral can alleviate the toxicity of heavy metals, though the mechanism is not entirely clear yet. Herein, we measured proteome, gene expression, enzyme activities, and selected sugars to investigate such effect thoroughly. Deep proteomic analysis revealed a minor impact of 100 µM Ni, 2.5 mM Si, or their combination on roots in 12-day-old hydroponically grown maize seedlings upon 9 days of exposure. Nonetheless, we suggested plausible mechanisms of Si mitigation of excessive Ni: Chelation by metallothioneins and phytochelatins, detoxification by glycine betaine pathway, and restructuring of plasma membrane transporters. Higher activity of glutathione S-transferase confirmed its plausible involvement in reducing Ni toxicity in combined treatment. Accumulation of sucrose synthase and corresponding soluble sugars in Ni and combined treatment implied high energy requirements both during heavy metal stress and its mitigation. Expression analysis of genes coding a few differentially accumulated proteins failed to reveal concordant changes, indicating posttranscriptional regulation. Proposed mitigation mechanisms should be functionally validated in follow-up studies.http://www.sciencedirect.com/science/article/pii/S0147651324014106Glycine betaineHeavy metalMineral amendmentProteomeSoluble sugarsZea mays |
| spellingShingle | Olha Lakhneko Ivana Fialová Roderik Fiala Mária Kopáčová Andrej Kováč Maksym Danchenko Silicon might mitigate nickel toxicity in maize roots via chelation, detoxification, and membrane transport Ecotoxicology and Environmental Safety Glycine betaine Heavy metal Mineral amendment Proteome Soluble sugars Zea mays |
| title | Silicon might mitigate nickel toxicity in maize roots via chelation, detoxification, and membrane transport |
| title_full | Silicon might mitigate nickel toxicity in maize roots via chelation, detoxification, and membrane transport |
| title_fullStr | Silicon might mitigate nickel toxicity in maize roots via chelation, detoxification, and membrane transport |
| title_full_unstemmed | Silicon might mitigate nickel toxicity in maize roots via chelation, detoxification, and membrane transport |
| title_short | Silicon might mitigate nickel toxicity in maize roots via chelation, detoxification, and membrane transport |
| title_sort | silicon might mitigate nickel toxicity in maize roots via chelation detoxification and membrane transport |
| topic | Glycine betaine Heavy metal Mineral amendment Proteome Soluble sugars Zea mays |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324014106 |
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