Protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo chorioallantoic membrane model
Summary: The chick embryo chorioallantoic membrane (CAM) is a viable alternative in vivo model to explore cancer biology due to its simplicity, accessibility, and cost-effectiveness. Here, we present a protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo CAM model....
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725000693 |
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| author | Tommy Chastel Serena Filiberti Stefania Mitola Roberto Ronca Andrei Turtoi Michela Corsini |
| author_facet | Tommy Chastel Serena Filiberti Stefania Mitola Roberto Ronca Andrei Turtoi Michela Corsini |
| author_sort | Tommy Chastel |
| collection | DOAJ |
| description | Summary: The chick embryo chorioallantoic membrane (CAM) is a viable alternative in vivo model to explore cancer biology due to its simplicity, accessibility, and cost-effectiveness. Here, we present a protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo CAM model. We describe steps for initial general preparation and for the angiogenic and tumorigenic assays in two distinct sections. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-5194e2eb5e7844ca9ec74a5411275bc6 |
| institution | OA Journals |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-5194e2eb5e7844ca9ec74a5411275bc62025-08-20T02:00:33ZengElsevierSTAR Protocols2666-16672025-03-016110366310.1016/j.xpro.2025.103663Protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo chorioallantoic membrane modelTommy Chastel0Serena Filiberti1Stefania Mitola2Roberto Ronca3Andrei Turtoi4Michela Corsini5Tumor Microenvironment and Resistance to Treatment Lab, INSERM U1194, 34090 Montpellier, FranceDepartment of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, ItalyDepartment of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, ItalyDepartment of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, ItalyTumor Microenvironment and Resistance to Treatment Lab, INSERM U1194, 34090 Montpellier, FranceDepartment of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy; Corresponding authorSummary: The chick embryo chorioallantoic membrane (CAM) is a viable alternative in vivo model to explore cancer biology due to its simplicity, accessibility, and cost-effectiveness. Here, we present a protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo CAM model. We describe steps for initial general preparation and for the angiogenic and tumorigenic assays in two distinct sections. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725000693Cell biologyCancerModel organisms |
| spellingShingle | Tommy Chastel Serena Filiberti Stefania Mitola Roberto Ronca Andrei Turtoi Michela Corsini Protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo chorioallantoic membrane model STAR Protocols Cell biology Cancer Model organisms |
| title | Protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo chorioallantoic membrane model |
| title_full | Protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo chorioallantoic membrane model |
| title_fullStr | Protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo chorioallantoic membrane model |
| title_full_unstemmed | Protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo chorioallantoic membrane model |
| title_short | Protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo chorioallantoic membrane model |
| title_sort | protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo chorioallantoic membrane model |
| topic | Cell biology Cancer Model organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725000693 |
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