Protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo chorioallantoic membrane model

Summary: The chick embryo chorioallantoic membrane (CAM) is a viable alternative in vivo model to explore cancer biology due to its simplicity, accessibility, and cost-effectiveness. Here, we present a protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo CAM model....

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Bibliographic Details
Main Authors: Tommy Chastel, Serena Filiberti, Stefania Mitola, Roberto Ronca, Andrei Turtoi, Michela Corsini
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725000693
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Summary:Summary: The chick embryo chorioallantoic membrane (CAM) is a viable alternative in vivo model to explore cancer biology due to its simplicity, accessibility, and cost-effectiveness. Here, we present a protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo CAM model. We describe steps for initial general preparation and for the angiogenic and tumorigenic assays in two distinct sections. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
ISSN:2666-1667