Two-photon all-optical neurophysiology for the dissection of larval zebrafish brain functional and effective connectivity
Abstract One of the most audacious goals of modern neuroscience is unraveling the complex web of causal relations underlying the activity of neuronal populations on a whole-brain scale. This endeavor, which was prohibitive only a couple of decades ago, has recently become within reach owing to the a...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-10-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-024-06731-3 |
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| author | Lapo Turrini Pietro Ricci Michele Sorelli Giuseppe de Vito Marco Marchetti Francesco Vanzi Francesco Saverio Pavone |
| author_facet | Lapo Turrini Pietro Ricci Michele Sorelli Giuseppe de Vito Marco Marchetti Francesco Vanzi Francesco Saverio Pavone |
| author_sort | Lapo Turrini |
| collection | DOAJ |
| description | Abstract One of the most audacious goals of modern neuroscience is unraveling the complex web of causal relations underlying the activity of neuronal populations on a whole-brain scale. This endeavor, which was prohibitive only a couple of decades ago, has recently become within reach owing to the advancements in optical methods and the advent of genetically encoded indicators/actuators. These techniques, applied to the translucent larval zebrafish have enabled recording and manipulation of the activity of extensive neuronal populations spanning the entire vertebrate brain. Here, we present a custom two-photon optical system that couples light-sheet imaging and 3D excitation with acousto-optic deflectors for simultaneous high-speed volumetric recording and optogenetic stimulation. By employing a zebrafish line with pan-neuronal expression of both the calcium reporter GCaMP6s and the red-shifted opsin ReaChR, we implemented a crosstalk-free, noninvasive all-optical approach and applied it to reconstruct the functional and effective connectivity of the left habenula. |
| format | Article |
| id | doaj-art-518da65fdc9746e09d0505c576bd9d4d |
| institution | Kabale University |
| issn | 2399-3642 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj-art-518da65fdc9746e09d0505c576bd9d4d2025-08-20T03:48:06ZengNature PortfolioCommunications Biology2399-36422024-10-017111610.1038/s42003-024-06731-3Two-photon all-optical neurophysiology for the dissection of larval zebrafish brain functional and effective connectivityLapo Turrini0Pietro Ricci1Michele Sorelli2Giuseppe de Vito3Marco Marchetti4Francesco Vanzi5Francesco Saverio Pavone6National Institute of Optics, National Research Council (INO-CNR)Department of Physics and Astronomy, University of FlorenceEuropean Laboratory for Non-linear Spectroscopy (LENS)National Institute of Optics, National Research Council (INO-CNR)L4T-Light4TechEuropean Laboratory for Non-linear Spectroscopy (LENS)National Institute of Optics, National Research Council (INO-CNR)Abstract One of the most audacious goals of modern neuroscience is unraveling the complex web of causal relations underlying the activity of neuronal populations on a whole-brain scale. This endeavor, which was prohibitive only a couple of decades ago, has recently become within reach owing to the advancements in optical methods and the advent of genetically encoded indicators/actuators. These techniques, applied to the translucent larval zebrafish have enabled recording and manipulation of the activity of extensive neuronal populations spanning the entire vertebrate brain. Here, we present a custom two-photon optical system that couples light-sheet imaging and 3D excitation with acousto-optic deflectors for simultaneous high-speed volumetric recording and optogenetic stimulation. By employing a zebrafish line with pan-neuronal expression of both the calcium reporter GCaMP6s and the red-shifted opsin ReaChR, we implemented a crosstalk-free, noninvasive all-optical approach and applied it to reconstruct the functional and effective connectivity of the left habenula.https://doi.org/10.1038/s42003-024-06731-3 |
| spellingShingle | Lapo Turrini Pietro Ricci Michele Sorelli Giuseppe de Vito Marco Marchetti Francesco Vanzi Francesco Saverio Pavone Two-photon all-optical neurophysiology for the dissection of larval zebrafish brain functional and effective connectivity Communications Biology |
| title | Two-photon all-optical neurophysiology for the dissection of larval zebrafish brain functional and effective connectivity |
| title_full | Two-photon all-optical neurophysiology for the dissection of larval zebrafish brain functional and effective connectivity |
| title_fullStr | Two-photon all-optical neurophysiology for the dissection of larval zebrafish brain functional and effective connectivity |
| title_full_unstemmed | Two-photon all-optical neurophysiology for the dissection of larval zebrafish brain functional and effective connectivity |
| title_short | Two-photon all-optical neurophysiology for the dissection of larval zebrafish brain functional and effective connectivity |
| title_sort | two photon all optical neurophysiology for the dissection of larval zebrafish brain functional and effective connectivity |
| url | https://doi.org/10.1038/s42003-024-06731-3 |
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