Phthalylglycyl chloride as a derivatization reagent for determination of urinary amino acids using ultra high-performance liquid chromatography coupled with high resolution mass spectrometry
A new derivatization reagent, phthalylglycyl chloride (PG-Cl), for the analysis of urinary amino acids was demonstrated using reversed-phase ultra-high performance liquid chromatography coupled to electrospray ionization-quadrupole-time-of-flight mass spectrometer. The study compares the limits of d...
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Elsevier
2024-11-01
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| Series: | Journal of Chromatography Open |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772391724000495 |
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| author | Azamat Temerdashev Pavel N. Nesterenko Sanka N. Atapattu Yu-Qi Feng Maria Zorina Kseniya Zhurkina Elina Gashimova Maxim O. Steshin Victor V. Dotsenko |
| author_facet | Azamat Temerdashev Pavel N. Nesterenko Sanka N. Atapattu Yu-Qi Feng Maria Zorina Kseniya Zhurkina Elina Gashimova Maxim O. Steshin Victor V. Dotsenko |
| author_sort | Azamat Temerdashev |
| collection | DOAJ |
| description | A new derivatization reagent, phthalylglycyl chloride (PG-Cl), for the analysis of urinary amino acids was demonstrated using reversed-phase ultra-high performance liquid chromatography coupled to electrospray ionization-quadrupole-time-of-flight mass spectrometer. The study compares the limits of detection and quantification for different sample preparation techniques with the commonly used and effective method of derivatization using dansyl chloride. The stability of the derivatives was investigated, and the study includes an example of urinary amino acid analysis using PG-Cl derivatization. The optimal values for pH, temperature, and concentration of the derivatization reagent were established. Limits of quantification in the range of 0.5–500 µg/mL were obtained for different amino acids. The possibility of phthalylglycyl chloride usage for non-target screening applications and targeted analysis of amino acids in urine was discussed. An advantages and issues of PG-Cl in comparison with commonly used acyl-chlorides (FMOC-Cl and DNS-Cl) provided and discussed. |
| format | Article |
| id | doaj-art-5178b873cd564966896ddec73c7c2736 |
| institution | OA Journals |
| issn | 2772-3917 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Chromatography Open |
| spelling | doaj-art-5178b873cd564966896ddec73c7c27362025-08-20T02:37:42ZengElsevierJournal of Chromatography Open2772-39172024-11-01610016210.1016/j.jcoa.2024.100162Phthalylglycyl chloride as a derivatization reagent for determination of urinary amino acids using ultra high-performance liquid chromatography coupled with high resolution mass spectrometryAzamat Temerdashev0Pavel N. Nesterenko1Sanka N. Atapattu2Yu-Qi Feng3Maria Zorina4Kseniya Zhurkina5Elina Gashimova6Maxim O. Steshin7Victor V. Dotsenko8Kuban State University, Stavropolskayast., 149, Krasnodar, 350040, Russia; Corresponding author.M.V. Lomonosov Moscow State University, Leninskie Gory, 1, Moscow, 119234, RussiaCanAm Bioresearch, Winnipeg, CanadaWuhan Textile University, Wuhan, ChinaKuban State University, Stavropolskayast., 149, Krasnodar, 350040, RussiaKuban State University, Stavropolskayast., 149, Krasnodar, 350040, RussiaKuban State University, Stavropolskayast., 149, Krasnodar, 350040, RussiaAlgimed-Techno, Moscow, RussiaKuban State University, Stavropolskayast., 149, Krasnodar, 350040, Russia; North Caucasus Federal University, Stavropol, 355009, RussiaA new derivatization reagent, phthalylglycyl chloride (PG-Cl), for the analysis of urinary amino acids was demonstrated using reversed-phase ultra-high performance liquid chromatography coupled to electrospray ionization-quadrupole-time-of-flight mass spectrometer. The study compares the limits of detection and quantification for different sample preparation techniques with the commonly used and effective method of derivatization using dansyl chloride. The stability of the derivatives was investigated, and the study includes an example of urinary amino acid analysis using PG-Cl derivatization. The optimal values for pH, temperature, and concentration of the derivatization reagent were established. Limits of quantification in the range of 0.5–500 µg/mL were obtained for different amino acids. The possibility of phthalylglycyl chloride usage for non-target screening applications and targeted analysis of amino acids in urine was discussed. An advantages and issues of PG-Cl in comparison with commonly used acyl-chlorides (FMOC-Cl and DNS-Cl) provided and discussed.http://www.sciencedirect.com/science/article/pii/S2772391724000495DerivatizationSample preparationPhthalylglycyl chlorideNon-targeted screeningUHPLC-MS/MSHRMS |
| spellingShingle | Azamat Temerdashev Pavel N. Nesterenko Sanka N. Atapattu Yu-Qi Feng Maria Zorina Kseniya Zhurkina Elina Gashimova Maxim O. Steshin Victor V. Dotsenko Phthalylglycyl chloride as a derivatization reagent for determination of urinary amino acids using ultra high-performance liquid chromatography coupled with high resolution mass spectrometry Journal of Chromatography Open Derivatization Sample preparation Phthalylglycyl chloride Non-targeted screening UHPLC-MS/MS HRMS |
| title | Phthalylglycyl chloride as a derivatization reagent for determination of urinary amino acids using ultra high-performance liquid chromatography coupled with high resolution mass spectrometry |
| title_full | Phthalylglycyl chloride as a derivatization reagent for determination of urinary amino acids using ultra high-performance liquid chromatography coupled with high resolution mass spectrometry |
| title_fullStr | Phthalylglycyl chloride as a derivatization reagent for determination of urinary amino acids using ultra high-performance liquid chromatography coupled with high resolution mass spectrometry |
| title_full_unstemmed | Phthalylglycyl chloride as a derivatization reagent for determination of urinary amino acids using ultra high-performance liquid chromatography coupled with high resolution mass spectrometry |
| title_short | Phthalylglycyl chloride as a derivatization reagent for determination of urinary amino acids using ultra high-performance liquid chromatography coupled with high resolution mass spectrometry |
| title_sort | phthalylglycyl chloride as a derivatization reagent for determination of urinary amino acids using ultra high performance liquid chromatography coupled with high resolution mass spectrometry |
| topic | Derivatization Sample preparation Phthalylglycyl chloride Non-targeted screening UHPLC-MS/MS HRMS |
| url | http://www.sciencedirect.com/science/article/pii/S2772391724000495 |
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