23- EXTRACTION OF POLYSACCHARIDES FROM THE LEAVES OF JEWS-MALLOW Corchorus olitorius L .AND THEIR POTENTIAL ANTICOAGULANT ACTIVITY
Polysaccharide was extracted from the leaves of (Molokhia) Jew’s-mallow by hotwater extraction using trichloro acetic acid(TCA) to removal of protein from the polysaccharide ,precipitation with ethanol, and obtained a polysaccharide yield from 4.2% based on wet weight source. The biochemical composi...
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College of Veterinary Medicine, University of Basrah, Iraq
2018-06-01
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| Series: | Basrah Journal of Veterinary Research |
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| Online Access: | https://bjvr.uobasrah.edu.iq/article_144957_2535d982d536e7a4ca01ae02acf00946.pdf |
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| author | Mohammed Zyarah Eskander |
| author_facet | Mohammed Zyarah Eskander |
| author_sort | Mohammed Zyarah Eskander |
| collection | DOAJ |
| description | Polysaccharide was extracted from the leaves of (Molokhia) Jew’s-mallow by hotwater extraction using trichloro acetic acid(TCA) to removal of protein from the
polysaccharide ,precipitation with ethanol, and obtained a polysaccharide yield from
4.2% based on wet weight source. The biochemical composition of the polysaccharide
contains total carbohydrate,sulfateandprotein77.6%, 8.6%, 0.63%, respectively.
Phytochemical tests were carried out for polysaccharide. The previous studies
indicated the presence of glycoside, phenol and tannin in polysaccharide from Jew’smallow. However, the presence of both alkaloids and saponins was not observed .The
Fourier transform infrared (FTIR) spectral analysis of the polysaccharides consist of
carboxyls and sulfate groups.The anticoagulant activity of polysaccharide was
evaluated by activated partial thromboplastin time (APTT) and prothrombin time (PT)
assays with respect to heparin The results obtained by APTT assay, through the
increase in coagulation time, the increase in blood coagulation time with increased
polysaccharide concentration, the 100 μg/ml polysaccharide concentration had the
longest time of 210Sce and was compatible with the concentration of1000 (IU/ ml) of
heparin.However the time of PT did not have any apparent effectiveness in increasing
the time of coagulation at all concentration compared with heparin. |
| format | Article |
| id | doaj-art-516f505fed5f4bf79917d5e76591a36f |
| institution | OA Journals |
| issn | 1813-8497 2410-8456 |
| language | English |
| publishDate | 2018-06-01 |
| publisher | College of Veterinary Medicine, University of Basrah, Iraq |
| record_format | Article |
| series | Basrah Journal of Veterinary Research |
| spelling | doaj-art-516f505fed5f4bf79917d5e76591a36f2025-08-20T01:49:57ZengCollege of Veterinary Medicine, University of Basrah, IraqBasrah Journal of Veterinary Research1813-84972410-84562018-06-0117127729010.33762/bvetr.2018.14495714495723- EXTRACTION OF POLYSACCHARIDES FROM THE LEAVES OF JEWS-MALLOW Corchorus olitorius L .AND THEIR POTENTIAL ANTICOAGULANT ACTIVITYMohammed Zyarah EskanderPolysaccharide was extracted from the leaves of (Molokhia) Jew’s-mallow by hotwater extraction using trichloro acetic acid(TCA) to removal of protein from the polysaccharide ,precipitation with ethanol, and obtained a polysaccharide yield from 4.2% based on wet weight source. The biochemical composition of the polysaccharide contains total carbohydrate,sulfateandprotein77.6%, 8.6%, 0.63%, respectively. Phytochemical tests were carried out for polysaccharide. The previous studies indicated the presence of glycoside, phenol and tannin in polysaccharide from Jew’smallow. However, the presence of both alkaloids and saponins was not observed .The Fourier transform infrared (FTIR) spectral analysis of the polysaccharides consist of carboxyls and sulfate groups.The anticoagulant activity of polysaccharide was evaluated by activated partial thromboplastin time (APTT) and prothrombin time (PT) assays with respect to heparin The results obtained by APTT assay, through the increase in coagulation time, the increase in blood coagulation time with increased polysaccharide concentration, the 100 μg/ml polysaccharide concentration had the longest time of 210Sce and was compatible with the concentration of1000 (IU/ ml) of heparin.However the time of PT did not have any apparent effectiveness in increasing the time of coagulation at all concentration compared with heparin.https://bjvr.uobasrah.edu.iq/article_144957_2535d982d536e7a4ca01ae02acf00946.pdfjew mallowpolysaccharideaptt |
| spellingShingle | Mohammed Zyarah Eskander 23- EXTRACTION OF POLYSACCHARIDES FROM THE LEAVES OF JEWS-MALLOW Corchorus olitorius L .AND THEIR POTENTIAL ANTICOAGULANT ACTIVITY Basrah Journal of Veterinary Research jew mallow polysaccharide aptt |
| title | 23- EXTRACTION OF POLYSACCHARIDES FROM THE LEAVES OF JEWS-MALLOW Corchorus olitorius L .AND THEIR POTENTIAL ANTICOAGULANT ACTIVITY |
| title_full | 23- EXTRACTION OF POLYSACCHARIDES FROM THE LEAVES OF JEWS-MALLOW Corchorus olitorius L .AND THEIR POTENTIAL ANTICOAGULANT ACTIVITY |
| title_fullStr | 23- EXTRACTION OF POLYSACCHARIDES FROM THE LEAVES OF JEWS-MALLOW Corchorus olitorius L .AND THEIR POTENTIAL ANTICOAGULANT ACTIVITY |
| title_full_unstemmed | 23- EXTRACTION OF POLYSACCHARIDES FROM THE LEAVES OF JEWS-MALLOW Corchorus olitorius L .AND THEIR POTENTIAL ANTICOAGULANT ACTIVITY |
| title_short | 23- EXTRACTION OF POLYSACCHARIDES FROM THE LEAVES OF JEWS-MALLOW Corchorus olitorius L .AND THEIR POTENTIAL ANTICOAGULANT ACTIVITY |
| title_sort | 23 extraction of polysaccharides from the leaves of jews mallow corchorus olitorius l and their potential anticoagulant activity |
| topic | jew mallow polysaccharide aptt |
| url | https://bjvr.uobasrah.edu.iq/article_144957_2535d982d536e7a4ca01ae02acf00946.pdf |
| work_keys_str_mv | AT mohammedzyaraheskander 23extractionofpolysaccharidesfromtheleavesofjewsmallowcorchorusolitoriuslandtheirpotentialanticoagulantactivity |