Validity and reliability of the step test to estimate maximal oxygen consumption in pediatric population

Abstract Cardiorespiratory fitness is the most important variable related to health and a strong predictor of mortality. However, it is rarely used in clinics due to costs, specialized equipment, space needs, and the requirements of expert staff such as an exercise physiologist, physician, or other...

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Main Authors: Gerardo Weisstaub, José González, Ignacio Orizola, Juan Borquez, Matías Monsalves-Alvarez, Lydia Lera, Rodrigo Troncoso, Carlos Sepúlveda
Format: Article
Language:English
Published: Nature Portfolio 2025-01-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-024-84336-w
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author Gerardo Weisstaub
José González
Ignacio Orizola
Juan Borquez
Matías Monsalves-Alvarez
Lydia Lera
Rodrigo Troncoso
Carlos Sepúlveda
author_facet Gerardo Weisstaub
José González
Ignacio Orizola
Juan Borquez
Matías Monsalves-Alvarez
Lydia Lera
Rodrigo Troncoso
Carlos Sepúlveda
author_sort Gerardo Weisstaub
collection DOAJ
description Abstract Cardiorespiratory fitness is the most important variable related to health and a strong predictor of mortality. However, it is rarely used in clinics due to costs, specialized equipment, space needs, and the requirements of expert staff such as an exercise physiologist, physician, or other health professional. This work aims to validate and test the reliability of a submaximal step test to estimate VO2max of 8-to 16-year-old pediatric populations as a simple and low-cost tool for clinical practice. A cross-sectional study included 242 children and adolescents (42.1% girls) aged 8–16. Cardiorespiratory fitness was determined by a maximal incremental test on a treadmill until exhaustion. The step test entailed maintaining a steady pace of 22 steps per minute for 3 min (60 bpm), with the heart rate being recorded at the end of the test. Nutritional status was computed through BMI z-score. A multiple linear regression model validated the step test and developed a new equation to predict VO2max, including the third-minute heart rate, weight, and height. The reliability among predicted and measured VO2max was assessed by Bland-Altman analysis. The mean age was 12.5 ± 2.6; 51.6% were overweight or obese. The cardiorespiratory fitness measured as VO2max was 35.01 ± 0.58 ml·min-1·kg−1. A robust correlation was observed between the predicted VO2max from the step test and the measured VO2max (r = 0.86, p < 0.001). Bland-Altman analysis indicated statistical concordance between predicted and measured VO2max. Our findings indicate that the step test protocol is valid and reliable for estimating VO2max in children and adolescents. Furthermore, the predictive equation is suitable for application among children aged 8–16.
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spelling doaj-art-516ea8479dc740d5949d5db84dff2bd52025-08-20T02:53:58ZengNature PortfolioScientific Reports2045-23222025-01-011511810.1038/s41598-024-84336-wValidity and reliability of the step test to estimate maximal oxygen consumption in pediatric populationGerardo Weisstaub0José González1Ignacio Orizola2Juan Borquez3Matías Monsalves-Alvarez4Lydia Lera5Rodrigo Troncoso6Carlos Sepúlveda7Laboratory of Nutritional Evaluation and Body Composition, Institute of Nutrition and Food Technology (INTA), University of ChileLaboratory of Nutrition and Physical Activity Research (LABINAF), Institute of Nutrition and Food Technology (INTA), University of ChileUnidad de Fisiología Clinica del Ejercicio, Centro de Especialidades Médicas y Odontológicas, Dirección de Salud IMFacultad de Ciencias de la Salud, Universidad de MagallanesExercise and Rehabilitation Sciences Institute, Faculty of Rehabilitation Sciences, Universidad Andres BelloLatin Division, Keiser UniversityLaboratory of Nutrition and Physical Activity Research (LABINAF), Institute of Nutrition and Food Technology (INTA), University of ChileLaboratory of Nutrition and Physical Activity Research (LABINAF), Institute of Nutrition and Food Technology (INTA), University of ChileAbstract Cardiorespiratory fitness is the most important variable related to health and a strong predictor of mortality. However, it is rarely used in clinics due to costs, specialized equipment, space needs, and the requirements of expert staff such as an exercise physiologist, physician, or other health professional. This work aims to validate and test the reliability of a submaximal step test to estimate VO2max of 8-to 16-year-old pediatric populations as a simple and low-cost tool for clinical practice. A cross-sectional study included 242 children and adolescents (42.1% girls) aged 8–16. Cardiorespiratory fitness was determined by a maximal incremental test on a treadmill until exhaustion. The step test entailed maintaining a steady pace of 22 steps per minute for 3 min (60 bpm), with the heart rate being recorded at the end of the test. Nutritional status was computed through BMI z-score. A multiple linear regression model validated the step test and developed a new equation to predict VO2max, including the third-minute heart rate, weight, and height. The reliability among predicted and measured VO2max was assessed by Bland-Altman analysis. The mean age was 12.5 ± 2.6; 51.6% were overweight or obese. The cardiorespiratory fitness measured as VO2max was 35.01 ± 0.58 ml·min-1·kg−1. A robust correlation was observed between the predicted VO2max from the step test and the measured VO2max (r = 0.86, p < 0.001). Bland-Altman analysis indicated statistical concordance between predicted and measured VO2max. Our findings indicate that the step test protocol is valid and reliable for estimating VO2max in children and adolescents. Furthermore, the predictive equation is suitable for application among children aged 8–16.https://doi.org/10.1038/s41598-024-84336-wCardiorespiratory fitnessChildrenExercise testingHeart rate.
spellingShingle Gerardo Weisstaub
José González
Ignacio Orizola
Juan Borquez
Matías Monsalves-Alvarez
Lydia Lera
Rodrigo Troncoso
Carlos Sepúlveda
Validity and reliability of the step test to estimate maximal oxygen consumption in pediatric population
Scientific Reports
Cardiorespiratory fitness
Children
Exercise testing
Heart rate.
title Validity and reliability of the step test to estimate maximal oxygen consumption in pediatric population
title_full Validity and reliability of the step test to estimate maximal oxygen consumption in pediatric population
title_fullStr Validity and reliability of the step test to estimate maximal oxygen consumption in pediatric population
title_full_unstemmed Validity and reliability of the step test to estimate maximal oxygen consumption in pediatric population
title_short Validity and reliability of the step test to estimate maximal oxygen consumption in pediatric population
title_sort validity and reliability of the step test to estimate maximal oxygen consumption in pediatric population
topic Cardiorespiratory fitness
Children
Exercise testing
Heart rate.
url https://doi.org/10.1038/s41598-024-84336-w
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