A cell-penetrating bispecific antibody suppresses hepatitis B virus replication and secretion
Hepatitis B virus (HBV) represents one of the major pathogenic factor that leads to chronic liver diseases and the development of hepatocellular carcinoma (HCC). The currently approved anti-HBV drugs cannot eradicate the virus or block the development of HCC. HBV nucleocapsid consists of the hepatit...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Virus Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0168170225000073 |
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| author | Chongwei Xie Bing Zhou Da Yao Xin Wang Lihong Zhong Chuanghua Qiu Junfang Zhang |
| author_facet | Chongwei Xie Bing Zhou Da Yao Xin Wang Lihong Zhong Chuanghua Qiu Junfang Zhang |
| author_sort | Chongwei Xie |
| collection | DOAJ |
| description | Hepatitis B virus (HBV) represents one of the major pathogenic factor that leads to chronic liver diseases and the development of hepatocellular carcinoma (HCC). The currently approved anti-HBV drugs cannot eradicate the virus or block the development of HCC. HBV nucleocapsid consists of the hepatitis B core antigen (HBcAg) and the HBV relaxed-circular partially double-stranded DNA (rcDNA), indispensable in virus replication. The present study reported a cell-penetrating bispecific antibody targeting HBcAg and preS1, fused with the cell-penetrating peptide R9TAT, named Anti-preS1 × Anti-HBcAg-R9TAT. The antibody could recognize preS1 and HBcAg and internalize into living cells efficiently, suppressing the extracellular hepatitis B surface antigen (HBsAg) and hepatitis B envelope antigen, and the intracellular HBsAg and HBcAg in vitro. This cell-penetrating bispecific antibody is a novel approach to suppressing HBV replication and secretion and is a promising anti-HBV therapeutic antibody candidate. |
| format | Article |
| id | doaj-art-5166cb61916b414cac32690bfecc331f |
| institution | Kabale University |
| issn | 1872-7492 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Virus Research |
| spelling | doaj-art-5166cb61916b414cac32690bfecc331f2025-02-02T05:26:39ZengElsevierVirus Research1872-74922025-03-01353199531A cell-penetrating bispecific antibody suppresses hepatitis B virus replication and secretionChongwei Xie0Bing Zhou1Da Yao2Xin Wang3Lihong Zhong4Chuanghua Qiu5Junfang Zhang6Medical Research Center, Yuebei People's Hospital, Shantou University Medical College, 512025, Shaoguan, China; Shenzhen Immuthy Biotech Co., Ltd, 518107, Shenzhen, Guangdong, ChinaInstitute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital, The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, 518112, Shenzhen, Guangdong, ChinaThe First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 518037, Shenzhen, Guangdong, ChinaInstitute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital, The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, 518112, Shenzhen, Guangdong, ChinaThe First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 518037, Shenzhen, Guangdong, ChinaThe First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 518037, Shenzhen, Guangdong, China; Corresponding author at: The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 3002 Sungang Road, Shenzhen, Guangdong, China.Medical Research Center, Yuebei People's Hospital, Shantou University Medical College, 512025, Shaoguan, China; Shenzhen Immuthy Biotech Co., Ltd, 518107, Shenzhen, Guangdong, China; Corresponding author at: Shenzhen Immuthy Biotech Co., Ltd, Shenzhen, China, Medical Research Center, Yuebei People's Hospital, Shantou University Medical College, 512025, 133 Huimin Road, Shaoguan, China.Hepatitis B virus (HBV) represents one of the major pathogenic factor that leads to chronic liver diseases and the development of hepatocellular carcinoma (HCC). The currently approved anti-HBV drugs cannot eradicate the virus or block the development of HCC. HBV nucleocapsid consists of the hepatitis B core antigen (HBcAg) and the HBV relaxed-circular partially double-stranded DNA (rcDNA), indispensable in virus replication. The present study reported a cell-penetrating bispecific antibody targeting HBcAg and preS1, fused with the cell-penetrating peptide R9TAT, named Anti-preS1 × Anti-HBcAg-R9TAT. The antibody could recognize preS1 and HBcAg and internalize into living cells efficiently, suppressing the extracellular hepatitis B surface antigen (HBsAg) and hepatitis B envelope antigen, and the intracellular HBsAg and HBcAg in vitro. This cell-penetrating bispecific antibody is a novel approach to suppressing HBV replication and secretion and is a promising anti-HBV therapeutic antibody candidate.http://www.sciencedirect.com/science/article/pii/S0168170225000073Cell-penetrating bispecific antibodyHBcAgCell-penetrating peptideHBV |
| spellingShingle | Chongwei Xie Bing Zhou Da Yao Xin Wang Lihong Zhong Chuanghua Qiu Junfang Zhang A cell-penetrating bispecific antibody suppresses hepatitis B virus replication and secretion Virus Research Cell-penetrating bispecific antibody HBcAg Cell-penetrating peptide HBV |
| title | A cell-penetrating bispecific antibody suppresses hepatitis B virus replication and secretion |
| title_full | A cell-penetrating bispecific antibody suppresses hepatitis B virus replication and secretion |
| title_fullStr | A cell-penetrating bispecific antibody suppresses hepatitis B virus replication and secretion |
| title_full_unstemmed | A cell-penetrating bispecific antibody suppresses hepatitis B virus replication and secretion |
| title_short | A cell-penetrating bispecific antibody suppresses hepatitis B virus replication and secretion |
| title_sort | cell penetrating bispecific antibody suppresses hepatitis b virus replication and secretion |
| topic | Cell-penetrating bispecific antibody HBcAg Cell-penetrating peptide HBV |
| url | http://www.sciencedirect.com/science/article/pii/S0168170225000073 |
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