Evaluation of the relationship between progression and SARS-CoV-2 viral load in COVID-19 cases in Ankara, Turkey

Introduction: Patients infected with SARS-CoV-2 may present with varying clinical pictures. This study aimed to examine the relationship between viral load cycle threshold value, clinical prognosis and other laboratory parameters in initial swab samples on the day of hospitalization. Methodology...

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Main Authors: Mustafa Guney, Tugrul Hosbul, Ferhat Cuce, Cumhur Artuk, Gurhan Taskin, Murat Caglayan, Sema Alacam, Muhammed Furkan Kurkcu, Fatime Yildiz, Harun Erdal, Gul Erdem, Ayfer Bakir
Format: Article
Language:English
Published: The Journal of Infection in Developing Countries 2022-03-01
Series:Journal of Infection in Developing Countries
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Online Access:https://jidc.org/index.php/journal/article/view/14940
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author Mustafa Guney
Tugrul Hosbul
Ferhat Cuce
Cumhur Artuk
Gurhan Taskin
Murat Caglayan
Sema Alacam
Muhammed Furkan Kurkcu
Fatime Yildiz
Harun Erdal
Gul Erdem
Ayfer Bakir
author_facet Mustafa Guney
Tugrul Hosbul
Ferhat Cuce
Cumhur Artuk
Gurhan Taskin
Murat Caglayan
Sema Alacam
Muhammed Furkan Kurkcu
Fatime Yildiz
Harun Erdal
Gul Erdem
Ayfer Bakir
author_sort Mustafa Guney
collection DOAJ
description Introduction: Patients infected with SARS-CoV-2 may present with varying clinical pictures. This study aimed to examine the relationship between viral load cycle threshold value, clinical prognosis and other laboratory parameters in initial swab samples on the day of hospitalization. Methodology: This retrospective and cross-sectional study included 112 patients, who were diagnosed with SARS-CoV-2 via the Bio-Rad CFX96 TouchTM system. Cycle threshold values for the RdRp gene obtained from reverse transcriptase polymerase chain reaction positive patients were recorded. Results: The mean age of the 112 patients was 47.57 ± 17 years. No relationship was found in symptoms, pneumonia, oxygen need, follow-up in intensive care unit, and mortality between patient groups with cycle threshold values of < 30 and ≥ 30. Frequencies of thrombocytopenia (50%) and elevated LDH levels were higher in patients with cycle threshold values of ≥ 30 (p = 0.02 and p = 0.04, respectively). There was a weak but significant correlation between cycle threshold values and CRP levels (Pearson’s r = 0.207, p = 0.029). Conclusions: Symptoms or clinical prognosis were not significantly related to the SARS-CoV-2 viral load levels tested at admission or for the first time within the scope of this study. Thrombocytopenia and elevated LDH rates were higher in patients with cycle threshold values of ≥ 30. A weak but significant correlation was found between the viral load and CRP levels. Large-scale studies are needed to further elucidate this subject matter.
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publisher The Journal of Infection in Developing Countries
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spelling doaj-art-513b708e2d8f488496a2a4ab675d4fc02025-08-20T03:52:42ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802022-03-01160310.3855/jidc.14940Evaluation of the relationship between progression and SARS-CoV-2 viral load in COVID-19 cases in Ankara, TurkeyMustafa Guney0Tugrul Hosbul1Ferhat Cuce2Cumhur Artuk3Gurhan Taskin4Murat Caglayan5Sema Alacam6Muhammed Furkan Kurkcu7Fatime Yildiz8Harun Erdal9Gul Erdem10Ayfer Bakir11University of Health Sciences, Gulhane Faculty of Medicine, Department of Microbiology, Ankara, TurkeyUniversity of Health Sciences, Gulhane Faculty of Medicine, Department of Microbiology, Ankara, TurkeyUniversity of Health Sciences, Gulhane Training and Research Hospital, Department of Radiology, Ankara, TurkeyUniversity of Health Sciences, Gulhane Faculty of Medicine, Department of Infectious Diseases and Clinical Microbiology, Ankara, TurkeyUniversity of Health Sciences, Gulhane Faculty of Medicine, Department of Critical Care, Ankara, TurkeyAnkara Provincial Health Directorate, Ministry of Health, Republic of Turkey, Ankara, TurkeyMinistry Of Health, Istanbul Education Research Hospital, Istanbul, TurkeyUniversity of Health Sciences, Gulhane Faculty of Medicine, Department of Microbiology, Ankara, TurkeyUniversity of Health Sciences, Gulhane Training and Research Hospital, Department of Microbiology, Ankara, TurkeyUniversity of Health Sciences, Gulhane Training and Research Hospital, Department of Gastroenterology, Ankara, TurkeyUniversity of Health Sciences, Diskapi Yildirim Beyazit Training and Research Hospital, Department of Microbiology, Ankara, TurkeyUniversity of Health Sciences, Gulhane Training and Research Hospital, Department of Microbiology, Ankara, Turkey Introduction: Patients infected with SARS-CoV-2 may present with varying clinical pictures. This study aimed to examine the relationship between viral load cycle threshold value, clinical prognosis and other laboratory parameters in initial swab samples on the day of hospitalization. Methodology: This retrospective and cross-sectional study included 112 patients, who were diagnosed with SARS-CoV-2 via the Bio-Rad CFX96 TouchTM system. Cycle threshold values for the RdRp gene obtained from reverse transcriptase polymerase chain reaction positive patients were recorded. Results: The mean age of the 112 patients was 47.57 ± 17 years. No relationship was found in symptoms, pneumonia, oxygen need, follow-up in intensive care unit, and mortality between patient groups with cycle threshold values of < 30 and ≥ 30. Frequencies of thrombocytopenia (50%) and elevated LDH levels were higher in patients with cycle threshold values of ≥ 30 (p = 0.02 and p = 0.04, respectively). There was a weak but significant correlation between cycle threshold values and CRP levels (Pearson’s r = 0.207, p = 0.029). Conclusions: Symptoms or clinical prognosis were not significantly related to the SARS-CoV-2 viral load levels tested at admission or for the first time within the scope of this study. Thrombocytopenia and elevated LDH rates were higher in patients with cycle threshold values of ≥ 30. A weak but significant correlation was found between the viral load and CRP levels. Large-scale studies are needed to further elucidate this subject matter. https://jidc.org/index.php/journal/article/view/14940SARS-CoV-2viral loadPCRprognosis
spellingShingle Mustafa Guney
Tugrul Hosbul
Ferhat Cuce
Cumhur Artuk
Gurhan Taskin
Murat Caglayan
Sema Alacam
Muhammed Furkan Kurkcu
Fatime Yildiz
Harun Erdal
Gul Erdem
Ayfer Bakir
Evaluation of the relationship between progression and SARS-CoV-2 viral load in COVID-19 cases in Ankara, Turkey
Journal of Infection in Developing Countries
SARS-CoV-2
viral load
PCR
prognosis
title Evaluation of the relationship between progression and SARS-CoV-2 viral load in COVID-19 cases in Ankara, Turkey
title_full Evaluation of the relationship between progression and SARS-CoV-2 viral load in COVID-19 cases in Ankara, Turkey
title_fullStr Evaluation of the relationship between progression and SARS-CoV-2 viral load in COVID-19 cases in Ankara, Turkey
title_full_unstemmed Evaluation of the relationship between progression and SARS-CoV-2 viral load in COVID-19 cases in Ankara, Turkey
title_short Evaluation of the relationship between progression and SARS-CoV-2 viral load in COVID-19 cases in Ankara, Turkey
title_sort evaluation of the relationship between progression and sars cov 2 viral load in covid 19 cases in ankara turkey
topic SARS-CoV-2
viral load
PCR
prognosis
url https://jidc.org/index.php/journal/article/view/14940
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