Glutathione Peroxidase 4 in Blunt Snout Bream (<i>Megalobrama amblycephala</i>) Regulates Ferroptosis and Inflammation in Response to <i>Aeromonas hydrophila</i> Infection
Glutathione peroxidase 4 (GPX4) plays a crucial role in regulating lipid peroxidation and is associated with infection and inflammation, particularly in terms of its effects on inflammatory cytokines and ferroptosis. This study aimed to investigate the regulatory effects of Gpx4 on the inflammatory...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
|
| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/47/5/326 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850127095022223360 |
|---|---|
| author | Miao He Huanling Wang Hong Liu |
| author_facet | Miao He Huanling Wang Hong Liu |
| author_sort | Miao He |
| collection | DOAJ |
| description | Glutathione peroxidase 4 (GPX4) plays a crucial role in regulating lipid peroxidation and is associated with infection and inflammation, particularly in terms of its effects on inflammatory cytokines and ferroptosis. This study aimed to investigate the regulatory effects of Gpx4 on the inflammatory response and ferroptosis in blunt snout bream (<i>Megalobrama amblycephala</i>), a significant freshwater fish species in China, after <i>Aeromonas hydrophila</i> infection. Using a bioinformatics analysis, we discovered that Gpx4 has a conserved protein structure and high amino acid identity in various carp species, indicating functional conservation across species and through involution. RT-qPCR analysis revealed that <i>gpx4</i> mRNA increased after the neuroembryonic stage during early development and was particularly highly expressed in the liver of healthy adult fish. Upon <i>A. hydrophila</i> infection, <i>gpx4</i> expression decreased significantly and rapidly in the liver. In L8824 cells, overexpression of <i>gpx4</i> suppressed inflammatory cytokines and inhibited ferroptosis in response to both <i>A. hydrophila</i> infection and induction of ferroptosis-inducer RSL3. These findings highlight the regulatory role of Gpx4 in cellular ferroptosis and inflammation, providing insights into the complex mechanisms of disease resistance and potentially aiding in the development of strategies for disease control in fish. |
| format | Article |
| id | doaj-art-513a159752414d5d9599131b71befdfa |
| institution | OA Journals |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj-art-513a159752414d5d9599131b71befdfa2025-08-20T02:33:44ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452025-05-0147532610.3390/cimb47050326Glutathione Peroxidase 4 in Blunt Snout Bream (<i>Megalobrama amblycephala</i>) Regulates Ferroptosis and Inflammation in Response to <i>Aeromonas hydrophila</i> InfectionMiao He0Huanling Wang1Hong Liu2Key Lab of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affair/Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaKey Lab of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affair/Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaKey Lab of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affair/Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaGlutathione peroxidase 4 (GPX4) plays a crucial role in regulating lipid peroxidation and is associated with infection and inflammation, particularly in terms of its effects on inflammatory cytokines and ferroptosis. This study aimed to investigate the regulatory effects of Gpx4 on the inflammatory response and ferroptosis in blunt snout bream (<i>Megalobrama amblycephala</i>), a significant freshwater fish species in China, after <i>Aeromonas hydrophila</i> infection. Using a bioinformatics analysis, we discovered that Gpx4 has a conserved protein structure and high amino acid identity in various carp species, indicating functional conservation across species and through involution. RT-qPCR analysis revealed that <i>gpx4</i> mRNA increased after the neuroembryonic stage during early development and was particularly highly expressed in the liver of healthy adult fish. Upon <i>A. hydrophila</i> infection, <i>gpx4</i> expression decreased significantly and rapidly in the liver. In L8824 cells, overexpression of <i>gpx4</i> suppressed inflammatory cytokines and inhibited ferroptosis in response to both <i>A. hydrophila</i> infection and induction of ferroptosis-inducer RSL3. These findings highlight the regulatory role of Gpx4 in cellular ferroptosis and inflammation, providing insights into the complex mechanisms of disease resistance and potentially aiding in the development of strategies for disease control in fish.https://www.mdpi.com/1467-3045/47/5/326<i>Megalobrama amblycephala</i><i>Aeromonas hydrophila</i><i>gpx4</i>ferroptosisinflammation |
| spellingShingle | Miao He Huanling Wang Hong Liu Glutathione Peroxidase 4 in Blunt Snout Bream (<i>Megalobrama amblycephala</i>) Regulates Ferroptosis and Inflammation in Response to <i>Aeromonas hydrophila</i> Infection Current Issues in Molecular Biology <i>Megalobrama amblycephala</i> <i>Aeromonas hydrophila</i> <i>gpx4</i> ferroptosis inflammation |
| title | Glutathione Peroxidase 4 in Blunt Snout Bream (<i>Megalobrama amblycephala</i>) Regulates Ferroptosis and Inflammation in Response to <i>Aeromonas hydrophila</i> Infection |
| title_full | Glutathione Peroxidase 4 in Blunt Snout Bream (<i>Megalobrama amblycephala</i>) Regulates Ferroptosis and Inflammation in Response to <i>Aeromonas hydrophila</i> Infection |
| title_fullStr | Glutathione Peroxidase 4 in Blunt Snout Bream (<i>Megalobrama amblycephala</i>) Regulates Ferroptosis and Inflammation in Response to <i>Aeromonas hydrophila</i> Infection |
| title_full_unstemmed | Glutathione Peroxidase 4 in Blunt Snout Bream (<i>Megalobrama amblycephala</i>) Regulates Ferroptosis and Inflammation in Response to <i>Aeromonas hydrophila</i> Infection |
| title_short | Glutathione Peroxidase 4 in Blunt Snout Bream (<i>Megalobrama amblycephala</i>) Regulates Ferroptosis and Inflammation in Response to <i>Aeromonas hydrophila</i> Infection |
| title_sort | glutathione peroxidase 4 in blunt snout bream i megalobrama amblycephala i regulates ferroptosis and inflammation in response to i aeromonas hydrophila i infection |
| topic | <i>Megalobrama amblycephala</i> <i>Aeromonas hydrophila</i> <i>gpx4</i> ferroptosis inflammation |
| url | https://www.mdpi.com/1467-3045/47/5/326 |
| work_keys_str_mv | AT miaohe glutathioneperoxidase4inbluntsnoutbreamimegalobramaamblycephalairegulatesferroptosisandinflammationinresponsetoiaeromonashydrophilaiinfection AT huanlingwang glutathioneperoxidase4inbluntsnoutbreamimegalobramaamblycephalairegulatesferroptosisandinflammationinresponsetoiaeromonashydrophilaiinfection AT hongliu glutathioneperoxidase4inbluntsnoutbreamimegalobramaamblycephalairegulatesferroptosisandinflammationinresponsetoiaeromonashydrophilaiinfection |