Immunization with a peptide containing MHC class I and II epitopes derived from the tumor antigen SIM2 induces an effective CD4 and CD8 T-cell response.
Here, we sought to determine whether peptide vaccines designed harbor both class I as well as class II restricted antigenic motifs could concurrently induce CD4 and CD8 T cell activation against autologous tumor antigens. Based on our prior genome-wide interrogation of human prostate cancer tissues...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0093231&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849720407977885696 |
|---|---|
| author | Haydn T Kissick Martin G Sanda Laura K Dunn Mohamed S Arredouani |
| author_facet | Haydn T Kissick Martin G Sanda Laura K Dunn Mohamed S Arredouani |
| author_sort | Haydn T Kissick |
| collection | DOAJ |
| description | Here, we sought to determine whether peptide vaccines designed harbor both class I as well as class II restricted antigenic motifs could concurrently induce CD4 and CD8 T cell activation against autologous tumor antigens. Based on our prior genome-wide interrogation of human prostate cancer tissues to identify genes over-expressed in cancer and absent in the periphery, we targeted SIM2 as a prototype autologous tumor antigen for these studies. Using humanized transgenic mice we found that the 9aa HLA-A*0201 epitope, SIM2(237-245), was effective at inducing an antigen specific response against SIM2-expressing prostate cancer cell line, PC3. Immunization with a multi-epitope peptide harboring both MHC-I and MHC-II restricted epitopes induced an IFN-γ response in CD8 T cells to the HLA-A*0201-restricted SIM2(237-245) epitope, and an IL-2 response by CD4 T cells to the SIM2(240-254) epitope. This peptide was also effective at inducing CD8+ T-cells that responded specifically to SIM2-expressing tumor cells. Collectively, the data presented in this study suggest that a single peptide containing multiple SIM2 epitopes can be used to induce both a CD4 and CD8 T cell response, providing a peptide-based vaccine formulation for potential use in immunotherapy of various cancers. |
| format | Article |
| id | doaj-art-51372bfb418a40bf819d2b00698f5e0f |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-51372bfb418a40bf819d2b00698f5e0f2025-08-20T03:11:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9323110.1371/journal.pone.0093231Immunization with a peptide containing MHC class I and II epitopes derived from the tumor antigen SIM2 induces an effective CD4 and CD8 T-cell response.Haydn T KissickMartin G SandaLaura K DunnMohamed S ArredouaniHere, we sought to determine whether peptide vaccines designed harbor both class I as well as class II restricted antigenic motifs could concurrently induce CD4 and CD8 T cell activation against autologous tumor antigens. Based on our prior genome-wide interrogation of human prostate cancer tissues to identify genes over-expressed in cancer and absent in the periphery, we targeted SIM2 as a prototype autologous tumor antigen for these studies. Using humanized transgenic mice we found that the 9aa HLA-A*0201 epitope, SIM2(237-245), was effective at inducing an antigen specific response against SIM2-expressing prostate cancer cell line, PC3. Immunization with a multi-epitope peptide harboring both MHC-I and MHC-II restricted epitopes induced an IFN-γ response in CD8 T cells to the HLA-A*0201-restricted SIM2(237-245) epitope, and an IL-2 response by CD4 T cells to the SIM2(240-254) epitope. This peptide was also effective at inducing CD8+ T-cells that responded specifically to SIM2-expressing tumor cells. Collectively, the data presented in this study suggest that a single peptide containing multiple SIM2 epitopes can be used to induce both a CD4 and CD8 T cell response, providing a peptide-based vaccine formulation for potential use in immunotherapy of various cancers.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0093231&type=printable |
| spellingShingle | Haydn T Kissick Martin G Sanda Laura K Dunn Mohamed S Arredouani Immunization with a peptide containing MHC class I and II epitopes derived from the tumor antigen SIM2 induces an effective CD4 and CD8 T-cell response. PLoS ONE |
| title | Immunization with a peptide containing MHC class I and II epitopes derived from the tumor antigen SIM2 induces an effective CD4 and CD8 T-cell response. |
| title_full | Immunization with a peptide containing MHC class I and II epitopes derived from the tumor antigen SIM2 induces an effective CD4 and CD8 T-cell response. |
| title_fullStr | Immunization with a peptide containing MHC class I and II epitopes derived from the tumor antigen SIM2 induces an effective CD4 and CD8 T-cell response. |
| title_full_unstemmed | Immunization with a peptide containing MHC class I and II epitopes derived from the tumor antigen SIM2 induces an effective CD4 and CD8 T-cell response. |
| title_short | Immunization with a peptide containing MHC class I and II epitopes derived from the tumor antigen SIM2 induces an effective CD4 and CD8 T-cell response. |
| title_sort | immunization with a peptide containing mhc class i and ii epitopes derived from the tumor antigen sim2 induces an effective cd4 and cd8 t cell response |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0093231&type=printable |
| work_keys_str_mv | AT haydntkissick immunizationwithapeptidecontainingmhcclassiandiiepitopesderivedfromthetumorantigensim2inducesaneffectivecd4andcd8tcellresponse AT martingsanda immunizationwithapeptidecontainingmhcclassiandiiepitopesderivedfromthetumorantigensim2inducesaneffectivecd4andcd8tcellresponse AT laurakdunn immunizationwithapeptidecontainingmhcclassiandiiepitopesderivedfromthetumorantigensim2inducesaneffectivecd4andcd8tcellresponse AT mohamedsarredouani immunizationwithapeptidecontainingmhcclassiandiiepitopesderivedfromthetumorantigensim2inducesaneffectivecd4andcd8tcellresponse |