Antiadhesive Property of Photoreactive Azidophenyl Low-Molecular-Weight Chitosan in Rabbit Laminotomy Model
Newly developed photoreactive azidophenyl chitosan (P-ALMC) has characteristics of a transformable gel type and its outer layer could be sealed up like a film after UV radiation. We aim to evaluate the antiadhesive properties of P-ALMC through comparing it with hyaluronic acid-carboxymethylcellulose...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/297159 |
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| author | Jong Won Kim Kwang-Sup Song Hyun Kang Eui-Chan Jang Mi Kyung Kim Tae Il Son |
| author_facet | Jong Won Kim Kwang-Sup Song Hyun Kang Eui-Chan Jang Mi Kyung Kim Tae Il Son |
| author_sort | Jong Won Kim |
| collection | DOAJ |
| description | Newly developed photoreactive azidophenyl chitosan (P-ALMC) has characteristics of a transformable gel type and its outer layer could be sealed up like a film after UV radiation. We aim to evaluate the antiadhesive properties of P-ALMC through comparing it with hyaluronic acid-carboxymethylcellulose membrane (HA-CMC) in a rabbit laminotomy model. Laminotomies were performed at the L3-4, L4-5, and L5-6 levels in 41 rabbits and each level was randomly assigned to either receive saline (group I), HA-CMC (group II), or P-ALMC (group III). The extent of peridural fibrosis, density of fibroblasts and inflammatory cells, and dural thickness were evaluated at 6 and 12 weeks postoperatively. In the groups II and III, the extents of peridural fibrosis and dural thickness were significantly smaller than those in group I (P<0.001) and no differences between groups II and III were found at the postoperative 6 and 12 weeks. There were no differences of cell density among groups. P-ALMC showed effective antiadhesive properties comparable to HA-CMC and could be one of the candidates as an anti-adhesive agent for spine surgery even further study is required to identify the effectiveness of its unique characteristics as mechanical barrier. |
| format | Article |
| id | doaj-art-51291c05eff74a4e8ffd5f4ef5b76ec4 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-51291c05eff74a4e8ffd5f4ef5b76ec42025-08-20T03:36:37ZengWileyJournal of Chemistry2090-90632090-90712013-01-01201310.1155/2013/297159297159Antiadhesive Property of Photoreactive Azidophenyl Low-Molecular-Weight Chitosan in Rabbit Laminotomy ModelJong Won Kim0Kwang-Sup Song1Hyun Kang2Eui-Chan Jang3Mi Kyung Kim4Tae Il Son5Department of Orthopaedic Surgery, Gang-Nam Himchan Hospital, 20-8 Songpa-dong, Songpa-gu, Seoul 138-170, Republic of KoreaDepartment of Orthopedic Surgery, College of Medicine, Chung-Ang University, Heukseok-dong, Dongjak-gu, Seoul 224-1, Republic of KoreaDepartment of Anesthesiology and Pain Medicine, College of Medicine, Chung-Ang University, Heukseok-dong, Dongjak-gu, Seoul 224-1, Republic of KoreaDepartment of Orthopedic Surgery, College of Medicine, Chung-Ang University, Heukseok-dong, Dongjak-gu, Seoul 224-1, Republic of KoreaDepartment of Pathology, College of Medicine, Chung-Ang University, Heukseok-dong, Dongjak-gu, Seoul 224-1, Republic of KoreaDepartment of Biotechnology and Bio-Environmental Technology (BET) Research Institute, Chung-Ang University, Gyeonggi-do 456-756, Republic of KoreaNewly developed photoreactive azidophenyl chitosan (P-ALMC) has characteristics of a transformable gel type and its outer layer could be sealed up like a film after UV radiation. We aim to evaluate the antiadhesive properties of P-ALMC through comparing it with hyaluronic acid-carboxymethylcellulose membrane (HA-CMC) in a rabbit laminotomy model. Laminotomies were performed at the L3-4, L4-5, and L5-6 levels in 41 rabbits and each level was randomly assigned to either receive saline (group I), HA-CMC (group II), or P-ALMC (group III). The extent of peridural fibrosis, density of fibroblasts and inflammatory cells, and dural thickness were evaluated at 6 and 12 weeks postoperatively. In the groups II and III, the extents of peridural fibrosis and dural thickness were significantly smaller than those in group I (P<0.001) and no differences between groups II and III were found at the postoperative 6 and 12 weeks. There were no differences of cell density among groups. P-ALMC showed effective antiadhesive properties comparable to HA-CMC and could be one of the candidates as an anti-adhesive agent for spine surgery even further study is required to identify the effectiveness of its unique characteristics as mechanical barrier.http://dx.doi.org/10.1155/2013/297159 |
| spellingShingle | Jong Won Kim Kwang-Sup Song Hyun Kang Eui-Chan Jang Mi Kyung Kim Tae Il Son Antiadhesive Property of Photoreactive Azidophenyl Low-Molecular-Weight Chitosan in Rabbit Laminotomy Model Journal of Chemistry |
| title | Antiadhesive Property of Photoreactive Azidophenyl Low-Molecular-Weight Chitosan in Rabbit Laminotomy Model |
| title_full | Antiadhesive Property of Photoreactive Azidophenyl Low-Molecular-Weight Chitosan in Rabbit Laminotomy Model |
| title_fullStr | Antiadhesive Property of Photoreactive Azidophenyl Low-Molecular-Weight Chitosan in Rabbit Laminotomy Model |
| title_full_unstemmed | Antiadhesive Property of Photoreactive Azidophenyl Low-Molecular-Weight Chitosan in Rabbit Laminotomy Model |
| title_short | Antiadhesive Property of Photoreactive Azidophenyl Low-Molecular-Weight Chitosan in Rabbit Laminotomy Model |
| title_sort | antiadhesive property of photoreactive azidophenyl low molecular weight chitosan in rabbit laminotomy model |
| url | http://dx.doi.org/10.1155/2013/297159 |
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