Some characteristics of the microbial community of reservoir fluids of the Romashkinskoe field
The condition of oil wells is reflected by the abundance and species composition of microorganisms in their reservoir fluids. However, such microorganisms are often present in small numbers, which hampers their analysis. The culture enrichment method is a good strategy to overcome this problem. Here...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Kazan Federal University
2022-06-01
|
| Series: | Учёные записки Казанского университета: Серия Естественные науки |
| Subjects: | |
| Online Access: | https://kpfu.ru/uz-eng-ns-2022-2-6.html |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850088828911484928 |
|---|---|
| author | L.R. Biktasheva S.Y. Selivanovskaya R.A. Mukhtarova H. Abdaljialil P.Y. Galitskaya |
| author_facet | L.R. Biktasheva S.Y. Selivanovskaya R.A. Mukhtarova H. Abdaljialil P.Y. Galitskaya |
| author_sort | L.R. Biktasheva |
| collection | DOAJ |
| description | The condition of oil wells is reflected by the abundance and species composition of microorganisms in their reservoir fluids. However, such microorganisms are often present in small numbers, which hampers their analysis. The culture enrichment method is a good strategy to overcome this problem. Here we discuss the results of a molecular biology-based comparative analysis of some characteristics of the microbial communities from the following two samples: the initial reservoir fluid sample (2313A) and the enriched sample (2313B).
The metagenomic analysis showed that sample 2313A was dominated by aerobic bacteria belonging to the genera Pseudomonas (order Pseudomonales) (47.5%) and Rhodococcus (order Actinomycetales) (45%). The community also comprised representatives of the anaerobic orders Halanaerobiales (1.4%) and Desulfovibrionales (0.03%). In sample 2313B, bacteria from the phylum Actinobacteria prevailed (99.9%) with Rhodococcus fascians (order Actinomycetales) identified as the dominant OTU. The quantitative real-time PCR analysis revealed that the enrichment process led to a 1063 and 244 times decrease in the relative abundance of copies of the rhlB and rhlAB genes encoding enzymes for the production of biosurfactants. The cultivation had a lesser effect on the abundance of copies of the dsrA and apsA genes, both involved in the sulfate reduction. When summarized, the obtained results indicate that the culture enrichment method enables the formation of a community differing substantially from the initial microbial communities of reservoir fluids of the oil wells. |
| format | Article |
| id | doaj-art-511c33a817d347cd8ad716ae8ad37559 |
| institution | DOAJ |
| issn | 2542-064X 2500-218X |
| language | English |
| publishDate | 2022-06-01 |
| publisher | Kazan Federal University |
| record_format | Article |
| series | Учёные записки Казанского университета: Серия Естественные науки |
| spelling | doaj-art-511c33a817d347cd8ad716ae8ad375592025-08-20T02:42:56ZengKazan Federal UniversityУчёные записки Казанского университета: Серия Естественные науки2542-064X2500-218X2022-06-01164226327810.26907/2542-064X.2022.2.263-278Some characteristics of the microbial community of reservoir fluids of the Romashkinskoe fieldL.R. Biktasheva0S.Y. Selivanovskaya1R.A. Mukhtarova2H. Abdaljialil3P.Y. Galitskaya4Kazan Federal University, Kazan, 420008 RussiaKazan Federal University, Kazan, 420008 RussiaKazan Federal University, Kazan, 420008 RussiaKazan Federal University, Kazan, 420008 RussiaKazan Federal University, Kazan, 420008 RussiaThe condition of oil wells is reflected by the abundance and species composition of microorganisms in their reservoir fluids. However, such microorganisms are often present in small numbers, which hampers their analysis. The culture enrichment method is a good strategy to overcome this problem. Here we discuss the results of a molecular biology-based comparative analysis of some characteristics of the microbial communities from the following two samples: the initial reservoir fluid sample (2313A) and the enriched sample (2313B). The metagenomic analysis showed that sample 2313A was dominated by aerobic bacteria belonging to the genera Pseudomonas (order Pseudomonales) (47.5%) and Rhodococcus (order Actinomycetales) (45%). The community also comprised representatives of the anaerobic orders Halanaerobiales (1.4%) and Desulfovibrionales (0.03%). In sample 2313B, bacteria from the phylum Actinobacteria prevailed (99.9%) with Rhodococcus fascians (order Actinomycetales) identified as the dominant OTU. The quantitative real-time PCR analysis revealed that the enrichment process led to a 1063 and 244 times decrease in the relative abundance of copies of the rhlB and rhlAB genes encoding enzymes for the production of biosurfactants. The cultivation had a lesser effect on the abundance of copies of the dsrA and apsA genes, both involved in the sulfate reduction. When summarized, the obtained results indicate that the culture enrichment method enables the formation of a community differing substantially from the initial microbial communities of reservoir fluids of the oil wells.https://kpfu.ru/uz-eng-ns-2022-2-6.htmlreservoir fluidbacterial communitysulfate reductionbiosurfactants |
| spellingShingle | L.R. Biktasheva S.Y. Selivanovskaya R.A. Mukhtarova H. Abdaljialil P.Y. Galitskaya Some characteristics of the microbial community of reservoir fluids of the Romashkinskoe field Учёные записки Казанского университета: Серия Естественные науки reservoir fluid bacterial community sulfate reduction biosurfactants |
| title | Some characteristics of the microbial community of reservoir fluids of the Romashkinskoe field |
| title_full | Some characteristics of the microbial community of reservoir fluids of the Romashkinskoe field |
| title_fullStr | Some characteristics of the microbial community of reservoir fluids of the Romashkinskoe field |
| title_full_unstemmed | Some characteristics of the microbial community of reservoir fluids of the Romashkinskoe field |
| title_short | Some characteristics of the microbial community of reservoir fluids of the Romashkinskoe field |
| title_sort | some characteristics of the microbial community of reservoir fluids of the romashkinskoe field |
| topic | reservoir fluid bacterial community sulfate reduction biosurfactants |
| url | https://kpfu.ru/uz-eng-ns-2022-2-6.html |
| work_keys_str_mv | AT lrbiktasheva somecharacteristicsofthemicrobialcommunityofreservoirfluidsoftheromashkinskoefield AT syselivanovskaya somecharacteristicsofthemicrobialcommunityofreservoirfluidsoftheromashkinskoefield AT ramukhtarova somecharacteristicsofthemicrobialcommunityofreservoirfluidsoftheromashkinskoefield AT habdaljialil somecharacteristicsofthemicrobialcommunityofreservoirfluidsoftheromashkinskoefield AT pygalitskaya somecharacteristicsofthemicrobialcommunityofreservoirfluidsoftheromashkinskoefield |