Apoptotic Cell Death and the Proliferative Capacity of Human Breast Cancers
The proliferative capacity (%S‐phase fraction), DNA ploidy, apoptosis frequency (DNA fragmentation) and steroid hormone receptor status (estrogen receptor, ER; progesterone receptor, PR) of 110 samples of human breast tissues with ductal invasive carcinoma were measured using biochemical and cytoflu...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
1998-01-01
|
| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/1998/906749 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832566814481055744 |
|---|---|
| author | Gabriele A. Losa Riccardo Graber |
| author_facet | Gabriele A. Losa Riccardo Graber |
| author_sort | Gabriele A. Losa |
| collection | DOAJ |
| description | The proliferative capacity (%S‐phase fraction), DNA ploidy, apoptosis frequency (DNA fragmentation) and steroid hormone receptor status (estrogen receptor, ER; progesterone receptor, PR) of 110 samples of human breast tissues with ductal invasive carcinoma were measured using biochemical and cytofluorimetric procedures. The DNA fragmentation had a left‐skewed frequency distribution and an overall median value of 1.64%, whilst the median %S‐phase fraction was 8%. The median %DNA fragmentation and %S‐phase fraction were 1.96% and 16% in hyperdiploid tumours (n=29; DNA index >1.1) higher than in hypodiploid tumors (n=10; DNA index 0.96), 0.38% and 7.5%. DNA diploid tumours (n=71) had median %DNA fragmentation and %S‐phase values of 1.68% and 6%, consistently lower than the median values of DNA hyperdiploid tumours. The ER content of hypodiploid tumours was about one half (median: 5.9 fmol/mg) the median values in hyperdiploid (10.6 fmol/mg) and diploid tumours (14.6 fmol/mg). This may correlate with the lowest frequency of apoptosis in hypodiploid tumours, at least when measured by biochemical methods which only detect cells in the late phases of apoptosis. In contrast, the median PR was lowest in hyperdiploid tumours than in hypo and/or diploid tumours. The %S‐phase/%fragmented DNA ratio for the hypodiploid tumours was 19.7, significantly higher than the ratios for hyperdiploid (8.2) and diploid tumours (3.6). These findings indicated that there is an imbalance between proliferative capacity and cell death or growth arrest in human breast tumours. This imbalance may well be linked to a loss of steroid hormone control. |
| format | Article |
| id | doaj-art-51155740561c4fab90ac4dcf0d541aad |
| institution | Kabale University |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 1998-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-51155740561c4fab90ac4dcf0d541aad2025-02-03T01:03:03ZengWileyAnalytical Cellular Pathology0921-89121878-36511998-01-0116111010.1155/1998/906749Apoptotic Cell Death and the Proliferative Capacity of Human Breast CancersGabriele A. Losa0Riccardo Graber1Laboratorio di Patologia Cellulare, Istituto cantonale di Patologia, CH‐6601 Locarno, SwitzerlandLaboratorio di Patologia Cellulare, Istituto cantonale di Patologia, CH‐6601 Locarno, SwitzerlandThe proliferative capacity (%S‐phase fraction), DNA ploidy, apoptosis frequency (DNA fragmentation) and steroid hormone receptor status (estrogen receptor, ER; progesterone receptor, PR) of 110 samples of human breast tissues with ductal invasive carcinoma were measured using biochemical and cytofluorimetric procedures. The DNA fragmentation had a left‐skewed frequency distribution and an overall median value of 1.64%, whilst the median %S‐phase fraction was 8%. The median %DNA fragmentation and %S‐phase fraction were 1.96% and 16% in hyperdiploid tumours (n=29; DNA index >1.1) higher than in hypodiploid tumors (n=10; DNA index 0.96), 0.38% and 7.5%. DNA diploid tumours (n=71) had median %DNA fragmentation and %S‐phase values of 1.68% and 6%, consistently lower than the median values of DNA hyperdiploid tumours. The ER content of hypodiploid tumours was about one half (median: 5.9 fmol/mg) the median values in hyperdiploid (10.6 fmol/mg) and diploid tumours (14.6 fmol/mg). This may correlate with the lowest frequency of apoptosis in hypodiploid tumours, at least when measured by biochemical methods which only detect cells in the late phases of apoptosis. In contrast, the median PR was lowest in hyperdiploid tumours than in hypo and/or diploid tumours. The %S‐phase/%fragmented DNA ratio for the hypodiploid tumours was 19.7, significantly higher than the ratios for hyperdiploid (8.2) and diploid tumours (3.6). These findings indicated that there is an imbalance between proliferative capacity and cell death or growth arrest in human breast tumours. This imbalance may well be linked to a loss of steroid hormone control.http://dx.doi.org/10.1155/1998/906749 |
| spellingShingle | Gabriele A. Losa Riccardo Graber Apoptotic Cell Death and the Proliferative Capacity of Human Breast Cancers Analytical Cellular Pathology |
| title | Apoptotic Cell Death and the Proliferative Capacity of Human Breast Cancers |
| title_full | Apoptotic Cell Death and the Proliferative Capacity of Human Breast Cancers |
| title_fullStr | Apoptotic Cell Death and the Proliferative Capacity of Human Breast Cancers |
| title_full_unstemmed | Apoptotic Cell Death and the Proliferative Capacity of Human Breast Cancers |
| title_short | Apoptotic Cell Death and the Proliferative Capacity of Human Breast Cancers |
| title_sort | apoptotic cell death and the proliferative capacity of human breast cancers |
| url | http://dx.doi.org/10.1155/1998/906749 |
| work_keys_str_mv | AT gabrielealosa apoptoticcelldeathandtheproliferativecapacityofhumanbreastcancers AT riccardograber apoptoticcelldeathandtheproliferativecapacityofhumanbreastcancers |