A novel zebrafish model to provide mechanistic insights into the inflammatory events in carrageenan-induced abdominal edema.
A suitable small animal model may help in the screening and evaluation of new drugs, especially those from natural products, which can be administered at lower dosages, fulfilling an urgent worldwide need. In this study, we explore whether zebrafish could be a model organism for carrageenan-induced...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0104414 |
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| author | Shi-Ying Huang Chien-Wei Feng Han-Chun Hung Chiranjib Chakraborty Chun-Hong Chen Wu-Fu Chen Yen-Hsuan Jean Hui-Min David Wang Chun-Sung Sung Yu-Min Sun Chang-Yi Wu Wangta Liu Chung-Der Hsiao Zhi-Hong Wen |
| author_facet | Shi-Ying Huang Chien-Wei Feng Han-Chun Hung Chiranjib Chakraborty Chun-Hong Chen Wu-Fu Chen Yen-Hsuan Jean Hui-Min David Wang Chun-Sung Sung Yu-Min Sun Chang-Yi Wu Wangta Liu Chung-Der Hsiao Zhi-Hong Wen |
| author_sort | Shi-Ying Huang |
| collection | DOAJ |
| description | A suitable small animal model may help in the screening and evaluation of new drugs, especially those from natural products, which can be administered at lower dosages, fulfilling an urgent worldwide need. In this study, we explore whether zebrafish could be a model organism for carrageenan-induced abdominal edema. The research results showed that intraperitoneal (i.p.) administration of 1.5% λ-carrageenan in a volume of 20 µL significantly increased abdominal edema in adult zebrafish. Levels of the proinflammatory proteins tumor necrosis factor-α (TNF-α) and inducible nitric oxide synthase (iNOS) were increased in carrageenan-injected adult zebrafish during the development of abdominal edema. An associated enhancement was also observed in the leukocyte marker, myeloperoxidase (MPO). To support these results, we further observed that i.p. methylprednisolone (MP; 1 µg), a positive control, significantly inhibited carrageenan-induced inflammation 24 h after carrageenan administration. Furthermore, i.p. pretreatment with either an anti-TNF-α antibody (1∶5 dilution in a volume of 20 µL) or the iNOS-selective inhibitor aminoguanidine (AG; 1 µg) inhibited carrageenan-induced abdominal edema in adult zebrafish. This new animal model is uncomplicated, easy to develop, and involves a straightforward inducement of inflammatory edema for the evaluation of small volumes of drugs or test compounds. |
| format | Article |
| id | doaj-art-5114e674de8d4106a6f85a85f907faee |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-5114e674de8d4106a6f85a85f907faee2025-08-20T02:22:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0198e10441410.1371/journal.pone.0104414A novel zebrafish model to provide mechanistic insights into the inflammatory events in carrageenan-induced abdominal edema.Shi-Ying HuangChien-Wei FengHan-Chun HungChiranjib ChakrabortyChun-Hong ChenWu-Fu ChenYen-Hsuan JeanHui-Min David WangChun-Sung SungYu-Min SunChang-Yi WuWangta LiuChung-Der HsiaoZhi-Hong WenA suitable small animal model may help in the screening and evaluation of new drugs, especially those from natural products, which can be administered at lower dosages, fulfilling an urgent worldwide need. In this study, we explore whether zebrafish could be a model organism for carrageenan-induced abdominal edema. The research results showed that intraperitoneal (i.p.) administration of 1.5% λ-carrageenan in a volume of 20 µL significantly increased abdominal edema in adult zebrafish. Levels of the proinflammatory proteins tumor necrosis factor-α (TNF-α) and inducible nitric oxide synthase (iNOS) were increased in carrageenan-injected adult zebrafish during the development of abdominal edema. An associated enhancement was also observed in the leukocyte marker, myeloperoxidase (MPO). To support these results, we further observed that i.p. methylprednisolone (MP; 1 µg), a positive control, significantly inhibited carrageenan-induced inflammation 24 h after carrageenan administration. Furthermore, i.p. pretreatment with either an anti-TNF-α antibody (1∶5 dilution in a volume of 20 µL) or the iNOS-selective inhibitor aminoguanidine (AG; 1 µg) inhibited carrageenan-induced abdominal edema in adult zebrafish. This new animal model is uncomplicated, easy to develop, and involves a straightforward inducement of inflammatory edema for the evaluation of small volumes of drugs or test compounds.https://doi.org/10.1371/journal.pone.0104414 |
| spellingShingle | Shi-Ying Huang Chien-Wei Feng Han-Chun Hung Chiranjib Chakraborty Chun-Hong Chen Wu-Fu Chen Yen-Hsuan Jean Hui-Min David Wang Chun-Sung Sung Yu-Min Sun Chang-Yi Wu Wangta Liu Chung-Der Hsiao Zhi-Hong Wen A novel zebrafish model to provide mechanistic insights into the inflammatory events in carrageenan-induced abdominal edema. PLoS ONE |
| title | A novel zebrafish model to provide mechanistic insights into the inflammatory events in carrageenan-induced abdominal edema. |
| title_full | A novel zebrafish model to provide mechanistic insights into the inflammatory events in carrageenan-induced abdominal edema. |
| title_fullStr | A novel zebrafish model to provide mechanistic insights into the inflammatory events in carrageenan-induced abdominal edema. |
| title_full_unstemmed | A novel zebrafish model to provide mechanistic insights into the inflammatory events in carrageenan-induced abdominal edema. |
| title_short | A novel zebrafish model to provide mechanistic insights into the inflammatory events in carrageenan-induced abdominal edema. |
| title_sort | novel zebrafish model to provide mechanistic insights into the inflammatory events in carrageenan induced abdominal edema |
| url | https://doi.org/10.1371/journal.pone.0104414 |
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