Development of Chitosan Nanoparticles as a Stable Drug Delivery System for Protein/siRNA
Chitosan nanoparticles (CS NPs) exhibit good physicochemical properties as drug delivery systems. The aim of this study is to determine the modulation of preparative parameters on the physical characteristics and colloidal stability of CS NPs. CS NPs were fabricated by ionic interaction with dextran...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2013/146320 |
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| author | Haliza Katas Maria Abdul Ghafoor Raja Kai Leong Lam |
| author_facet | Haliza Katas Maria Abdul Ghafoor Raja Kai Leong Lam |
| author_sort | Haliza Katas |
| collection | DOAJ |
| description | Chitosan nanoparticles (CS NPs) exhibit good physicochemical properties as drug delivery systems. The aim of this study is to determine the modulation of preparative parameters on the physical characteristics and colloidal stability of CS NPs. CS NPs were fabricated by ionic interaction with dextran sulphate (DS) prior to determination of their storage stability. The smallest CS NPs of 353±23 nm with a surface charge of +56.2±1.5 mV were produced when CS and DS were mixed at pH 4 and with a DS : CS mass ratio of 0.5 : 1. An entrapment efficiency of 98% was achieved when BSA/siRNA was loaded into the nanoparticles. The results also showed that particle size and surface charge of CS NPs were slightly changed up to 2 weeks when stored at 4°C. Greater particle size and surface charge were obtained with increasing the concentration of DS. In conclusion, NPs were sufficiently stable when kept at 4°C and able to carry and protect protein. |
| format | Article |
| id | doaj-art-50ff6618c1c04cb693ff44355d7810c5 |
| institution | Kabale University |
| issn | 1687-8787 1687-8795 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomaterials |
| spelling | doaj-art-50ff6618c1c04cb693ff44355d7810c52025-02-03T01:11:36ZengWileyInternational Journal of Biomaterials1687-87871687-87952013-01-01201310.1155/2013/146320146320Development of Chitosan Nanoparticles as a Stable Drug Delivery System for Protein/siRNAHaliza Katas0Maria Abdul Ghafoor Raja1Kai Leong Lam2Centre for Drug Delivery Research, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, MalaysiaCentre for Drug Delivery Research, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, MalaysiaCentre for Drug Delivery Research, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, MalaysiaChitosan nanoparticles (CS NPs) exhibit good physicochemical properties as drug delivery systems. The aim of this study is to determine the modulation of preparative parameters on the physical characteristics and colloidal stability of CS NPs. CS NPs were fabricated by ionic interaction with dextran sulphate (DS) prior to determination of their storage stability. The smallest CS NPs of 353±23 nm with a surface charge of +56.2±1.5 mV were produced when CS and DS were mixed at pH 4 and with a DS : CS mass ratio of 0.5 : 1. An entrapment efficiency of 98% was achieved when BSA/siRNA was loaded into the nanoparticles. The results also showed that particle size and surface charge of CS NPs were slightly changed up to 2 weeks when stored at 4°C. Greater particle size and surface charge were obtained with increasing the concentration of DS. In conclusion, NPs were sufficiently stable when kept at 4°C and able to carry and protect protein.http://dx.doi.org/10.1155/2013/146320 |
| spellingShingle | Haliza Katas Maria Abdul Ghafoor Raja Kai Leong Lam Development of Chitosan Nanoparticles as a Stable Drug Delivery System for Protein/siRNA International Journal of Biomaterials |
| title | Development of Chitosan Nanoparticles as a Stable Drug Delivery System for Protein/siRNA |
| title_full | Development of Chitosan Nanoparticles as a Stable Drug Delivery System for Protein/siRNA |
| title_fullStr | Development of Chitosan Nanoparticles as a Stable Drug Delivery System for Protein/siRNA |
| title_full_unstemmed | Development of Chitosan Nanoparticles as a Stable Drug Delivery System for Protein/siRNA |
| title_short | Development of Chitosan Nanoparticles as a Stable Drug Delivery System for Protein/siRNA |
| title_sort | development of chitosan nanoparticles as a stable drug delivery system for protein sirna |
| url | http://dx.doi.org/10.1155/2013/146320 |
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