Rapid Determination of 107 Illegally Added Nafils in Foods by Ultra-high Performance Liquid Chromatography-High Resolution Mass Spectrometry
A rapid method for the determination 107 illegally added nafils in compressed candies, honey and beverage was developed by ultra-high performance liquid chromatography coupled with quadrupole orbitrap high-resolution mass spectrometry. The samples were extracted with methanol and separated on a Wate...
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China Food Publishing Company
2025-05-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-10-032.pdf |
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| author | FENG Feng, CHANG Qizhi, SUN Xiao, DU Rongzhu, ZHANG Feng |
| author_facet | FENG Feng, CHANG Qizhi, SUN Xiao, DU Rongzhu, ZHANG Feng |
| author_sort | FENG Feng, CHANG Qizhi, SUN Xiao, DU Rongzhu, ZHANG Feng |
| collection | DOAJ |
| description | A rapid method for the determination 107 illegally added nafils in compressed candies, honey and beverage was developed by ultra-high performance liquid chromatography coupled with quadrupole orbitrap high-resolution mass spectrometry. The samples were extracted with methanol and separated on a Waters Acquity UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 μm) with gradient elution using a mobile phase consisting of acetonitrile and 0.1% formic acid in water. The detection was conducted in both positive and negative ion modes, and a full MS scan/data dependent MS2 mode was used to collect data. The 107 nafils showed good linearity in the concentration range of 4–300 μg/L, with correlation coefficients (R2) greater than 0.99. The limits of detection (LODs) were 0.1–1 mg/kg, and the limits of quantification (LOQs) were 0.2–2 mg/kg. The average recoveries ranged from 82.5% to 117.0% at three spiked levels of 2, 5, 10 mg/kg, with relative standard deviations of 0.9%–10.0% (n = 6). This method was fast, accurate, sensitive, and suitable for the rapid identification and quantification of illegal additives in foods. |
| format | Article |
| id | doaj-art-50fd9bc766ab4b52a7569cff5bddd22e |
| institution | Kabale University |
| issn | 1002-6630 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-50fd9bc766ab4b52a7569cff5bddd22e2025-08-20T03:25:07ZengChina Food Publishing CompanyShipin Kexue1002-66302025-05-01461029029810.7506/spkx1002-6630-20240808-067Rapid Determination of 107 Illegally Added Nafils in Foods by Ultra-high Performance Liquid Chromatography-High Resolution Mass SpectrometryFENG Feng, CHANG Qizhi, SUN Xiao, DU Rongzhu, ZHANG Feng0(1. Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100176, China; 2. Key Laboratory of Food Quality and Safety, State Administration for Market Regulation, Beijing 100176, China; 3. School of Pharmacy, China Medical University, Shenyang 110122, China; 4. Xi’an Institute for Food and Drug Control, Xi’an 710119, China)A rapid method for the determination 107 illegally added nafils in compressed candies, honey and beverage was developed by ultra-high performance liquid chromatography coupled with quadrupole orbitrap high-resolution mass spectrometry. The samples were extracted with methanol and separated on a Waters Acquity UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 μm) with gradient elution using a mobile phase consisting of acetonitrile and 0.1% formic acid in water. The detection was conducted in both positive and negative ion modes, and a full MS scan/data dependent MS2 mode was used to collect data. The 107 nafils showed good linearity in the concentration range of 4–300 μg/L, with correlation coefficients (R2) greater than 0.99. The limits of detection (LODs) were 0.1–1 mg/kg, and the limits of quantification (LOQs) were 0.2–2 mg/kg. The average recoveries ranged from 82.5% to 117.0% at three spiked levels of 2, 5, 10 mg/kg, with relative standard deviations of 0.9%–10.0% (n = 6). This method was fast, accurate, sensitive, and suitable for the rapid identification and quantification of illegal additives in foods.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-10-032.pdfultra-high performance liquid chromatography; high-resolution mass spectrometry; illegal additives; nafils |
| spellingShingle | FENG Feng, CHANG Qizhi, SUN Xiao, DU Rongzhu, ZHANG Feng Rapid Determination of 107 Illegally Added Nafils in Foods by Ultra-high Performance Liquid Chromatography-High Resolution Mass Spectrometry Shipin Kexue ultra-high performance liquid chromatography; high-resolution mass spectrometry; illegal additives; nafils |
| title | Rapid Determination of 107 Illegally Added Nafils in Foods by Ultra-high Performance Liquid Chromatography-High Resolution Mass Spectrometry |
| title_full | Rapid Determination of 107 Illegally Added Nafils in Foods by Ultra-high Performance Liquid Chromatography-High Resolution Mass Spectrometry |
| title_fullStr | Rapid Determination of 107 Illegally Added Nafils in Foods by Ultra-high Performance Liquid Chromatography-High Resolution Mass Spectrometry |
| title_full_unstemmed | Rapid Determination of 107 Illegally Added Nafils in Foods by Ultra-high Performance Liquid Chromatography-High Resolution Mass Spectrometry |
| title_short | Rapid Determination of 107 Illegally Added Nafils in Foods by Ultra-high Performance Liquid Chromatography-High Resolution Mass Spectrometry |
| title_sort | rapid determination of 107 illegally added nafils in foods by ultra high performance liquid chromatography high resolution mass spectrometry |
| topic | ultra-high performance liquid chromatography; high-resolution mass spectrometry; illegal additives; nafils |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-10-032.pdf |
| work_keys_str_mv | AT fengfengchangqizhisunxiaodurongzhuzhangfeng rapiddeterminationof107illegallyaddednafilsinfoodsbyultrahighperformanceliquidchromatographyhighresolutionmassspectrometry |