Rapid Determination of 107 Illegally Added Nafils in Foods by Ultra-high Performance Liquid Chromatography-High Resolution Mass Spectrometry
A rapid method for the determination 107 illegally added nafils in compressed candies, honey and beverage was developed by ultra-high performance liquid chromatography coupled with quadrupole orbitrap high-resolution mass spectrometry. The samples were extracted with methanol and separated on a Wate...
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| Main Author: | |
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| Format: | Article |
| Language: | English |
| Published: |
China Food Publishing Company
2025-05-01
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| Series: | Shipin Kexue |
| Subjects: | |
| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-10-032.pdf |
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| Summary: | A rapid method for the determination 107 illegally added nafils in compressed candies, honey and beverage was developed by ultra-high performance liquid chromatography coupled with quadrupole orbitrap high-resolution mass spectrometry. The samples were extracted with methanol and separated on a Waters Acquity UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 μm) with gradient elution using a mobile phase consisting of acetonitrile and 0.1% formic acid in water. The detection was conducted in both positive and negative ion modes, and a full MS scan/data dependent MS2 mode was used to collect data. The 107 nafils showed good linearity in the concentration range of 4–300 μg/L, with correlation coefficients (R2) greater than 0.99. The limits of detection (LODs) were 0.1–1 mg/kg, and the limits of quantification (LOQs) were 0.2–2 mg/kg. The average recoveries ranged from 82.5% to 117.0% at three spiked levels of 2, 5, 10 mg/kg, with relative standard deviations of 0.9%–10.0% (n = 6). This method was fast, accurate, sensitive, and suitable for the rapid identification and quantification of illegal additives in foods. |
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| ISSN: | 1002-6630 |