An Improved Test Method for Assaying the Inhibition of Bioflavonoids on Xanthine Oxidase Activity in vitro
Abstract The difference on inhibitory effects of bioflavonoids inhibiting XOD activity assayed by varying test methods cause of us to be further in consideration. The reported test method creating a micro‐environment surrounding XOD in the absence of ⋅O2−, which is seemly different from the assay in...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2024-12-01
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| Series: | ChemistryOpen |
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| Online Access: | https://doi.org/10.1002/open.202400127 |
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| author | Yuanyong Yao Tao Wu Meng Zhang Daihua Fu Hai Yang Shixue Chen |
| author_facet | Yuanyong Yao Tao Wu Meng Zhang Daihua Fu Hai Yang Shixue Chen |
| author_sort | Yuanyong Yao |
| collection | DOAJ |
| description | Abstract The difference on inhibitory effects of bioflavonoids inhibiting XOD activity assayed by varying test methods cause of us to be further in consideration. The reported test method creating a micro‐environment surrounding XOD in the absence of ⋅O2−, which is seemly different from the assay in vivo. So, the vitro test method for assaying XOD activity is necessary to be improved for selection of potential inhibitors in the presence of ⋅O2−. The inhibitory results demonstrated that bioflavonoids of MY, DMY, QUE and LUT are capable to be on effective IC50 values, but others are not. As well, their resulting inhibitions determined by the improved test method are much less than that reported in the literature, indicating that their chemical affinities with XOD become weaker. Moreover, DMY assayed on the inhibitions of XOD in the improved test method performs to be a better inhibitor, as compared to the assay of the reported test methods. Abasing on the transformation of DMY into MY in the presence of ⋅O2−, the good inhibition of DMY on XOD activity can be explained by the synergistic effect of MY. |
| format | Article |
| id | doaj-art-50f9ff996cf449599b3f50757f5bebf3 |
| institution | OA Journals |
| issn | 2191-1363 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | ChemistryOpen |
| spelling | doaj-art-50f9ff996cf449599b3f50757f5bebf32025-08-20T02:30:37ZengWiley-VCHChemistryOpen2191-13632024-12-011312n/an/a10.1002/open.202400127An Improved Test Method for Assaying the Inhibition of Bioflavonoids on Xanthine Oxidase Activity in vitroYuanyong Yao0Tao Wu1Meng Zhang2Daihua Fu3Hai Yang4Shixue Chen5State Ethnic Affairs Commission Key Development Laboratory of Chinese Veterinary Medicine & National and Local Joint Engineering Center of Chinese Veterinary Medicine Separation and Purification Technology Tongren Vocational and Technical University Tongren 554300 ChinaInstitute of Material and Chemical Engineering Tongren University Tongren 554300 ChinaInstitute of Material and Chemical Engineering Tongren University Tongren 554300 ChinaInstitute of Material and Chemical Engineering Tongren University Tongren 554300 ChinaInstitute of Material and Chemical Engineering Tongren University Tongren 554300 ChinaInstitute of Material and Chemical Engineering Tongren University Tongren 554300 ChinaAbstract The difference on inhibitory effects of bioflavonoids inhibiting XOD activity assayed by varying test methods cause of us to be further in consideration. The reported test method creating a micro‐environment surrounding XOD in the absence of ⋅O2−, which is seemly different from the assay in vivo. So, the vitro test method for assaying XOD activity is necessary to be improved for selection of potential inhibitors in the presence of ⋅O2−. The inhibitory results demonstrated that bioflavonoids of MY, DMY, QUE and LUT are capable to be on effective IC50 values, but others are not. As well, their resulting inhibitions determined by the improved test method are much less than that reported in the literature, indicating that their chemical affinities with XOD become weaker. Moreover, DMY assayed on the inhibitions of XOD in the improved test method performs to be a better inhibitor, as compared to the assay of the reported test methods. Abasing on the transformation of DMY into MY in the presence of ⋅O2−, the good inhibition of DMY on XOD activity can be explained by the synergistic effect of MY.https://doi.org/10.1002/open.202400127Test methodXanthine oxidaseBioflavonoidsIn vitro inhibition |
| spellingShingle | Yuanyong Yao Tao Wu Meng Zhang Daihua Fu Hai Yang Shixue Chen An Improved Test Method for Assaying the Inhibition of Bioflavonoids on Xanthine Oxidase Activity in vitro ChemistryOpen Test method Xanthine oxidase Bioflavonoids In vitro inhibition |
| title | An Improved Test Method for Assaying the Inhibition of Bioflavonoids on Xanthine Oxidase Activity in vitro |
| title_full | An Improved Test Method for Assaying the Inhibition of Bioflavonoids on Xanthine Oxidase Activity in vitro |
| title_fullStr | An Improved Test Method for Assaying the Inhibition of Bioflavonoids on Xanthine Oxidase Activity in vitro |
| title_full_unstemmed | An Improved Test Method for Assaying the Inhibition of Bioflavonoids on Xanthine Oxidase Activity in vitro |
| title_short | An Improved Test Method for Assaying the Inhibition of Bioflavonoids on Xanthine Oxidase Activity in vitro |
| title_sort | improved test method for assaying the inhibition of bioflavonoids on xanthine oxidase activity in vitro |
| topic | Test method Xanthine oxidase Bioflavonoids In vitro inhibition |
| url | https://doi.org/10.1002/open.202400127 |
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