Gibberellin Treatment Accelerates Starch Decomposition and Seed Germination in Sticky Nightshade (<i>Solanum sisymbriifolium</i> Lam.)

Sticky nightshade (<i>Solanum sisymbriifolium</i> Lam.) is a spiny species with a variety of disease resistance characteristics found worldwide within the <i>Solanum</i> genus. However, its low germination rate and long germination period pose obstacles to the effective use o...

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Main Authors: Haobo Xu, Danni Mo, Xingyu Zhang, Fangman Li, Jinbao Tao, Pingfei Ge, Yang Yang, Ziyuan Wang, Yuyang Zhang
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Horticulturae
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Online Access:https://www.mdpi.com/2311-7524/10/12/1342
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author Haobo Xu
Danni Mo
Xingyu Zhang
Fangman Li
Jinbao Tao
Pingfei Ge
Yang Yang
Ziyuan Wang
Yuyang Zhang
author_facet Haobo Xu
Danni Mo
Xingyu Zhang
Fangman Li
Jinbao Tao
Pingfei Ge
Yang Yang
Ziyuan Wang
Yuyang Zhang
author_sort Haobo Xu
collection DOAJ
description Sticky nightshade (<i>Solanum sisymbriifolium</i> Lam.) is a spiny species with a variety of disease resistance characteristics found worldwide within the <i>Solanum</i> genus. However, its low germination rate and long germination period pose obstacles to the effective use of this species. Here, we treated Sticky nightshade with different concentrations of gibberellin (GA<sub>3</sub>) and observed paraffin sections of Sticky nightshade seeds treated with different GA<sub>3</sub> concentrations over different time ranges. The results showed that a 400 mg/L exogenous GA<sub>3</sub> concentration at room temperature could improve the germination rate of Sticky nightshade the most effectively. Exogenous GA<sub>3</sub> treatment can significantly accelerate the hydrolysis of starch granules and increase the germination rate of seeds. Subsequently, we also measured the MDA content of Sticky nightshade seeds treated with different GA<sub>3</sub> concentrations over different time ranges. The result reveals that GA<sub>3</sub> treatment can steadily decrease Sticky nightshade seeds’ MDA content during germination, indicating that exogenous GA<sub>3</sub> treatment also reduces membrane peroxidation and maintains the stability of the plasma membrane. In this paper, we identified an optimal GA<sub>3</sub>-treated concentration of Sticky nightshade to improve seed germination at room temperature and explored the reason why the exogenous GA<sub>3</sub> treatment of Sticky nightshade seed increased the germination rate.
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spelling doaj-art-50f2d1afd18f4bb0afa04810792327b52025-08-20T02:00:23ZengMDPI AGHorticulturae2311-75242024-12-011012134210.3390/horticulturae10121342Gibberellin Treatment Accelerates Starch Decomposition and Seed Germination in Sticky Nightshade (<i>Solanum sisymbriifolium</i> Lam.)Haobo Xu0Danni Mo1Xingyu Zhang2Fangman Li3Jinbao Tao4Pingfei Ge5Yang Yang6Ziyuan Wang7Yuyang Zhang8National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan 430070, ChinaSticky nightshade (<i>Solanum sisymbriifolium</i> Lam.) is a spiny species with a variety of disease resistance characteristics found worldwide within the <i>Solanum</i> genus. However, its low germination rate and long germination period pose obstacles to the effective use of this species. Here, we treated Sticky nightshade with different concentrations of gibberellin (GA<sub>3</sub>) and observed paraffin sections of Sticky nightshade seeds treated with different GA<sub>3</sub> concentrations over different time ranges. The results showed that a 400 mg/L exogenous GA<sub>3</sub> concentration at room temperature could improve the germination rate of Sticky nightshade the most effectively. Exogenous GA<sub>3</sub> treatment can significantly accelerate the hydrolysis of starch granules and increase the germination rate of seeds. Subsequently, we also measured the MDA content of Sticky nightshade seeds treated with different GA<sub>3</sub> concentrations over different time ranges. The result reveals that GA<sub>3</sub> treatment can steadily decrease Sticky nightshade seeds’ MDA content during germination, indicating that exogenous GA<sub>3</sub> treatment also reduces membrane peroxidation and maintains the stability of the plasma membrane. In this paper, we identified an optimal GA<sub>3</sub>-treated concentration of Sticky nightshade to improve seed germination at room temperature and explored the reason why the exogenous GA<sub>3</sub> treatment of Sticky nightshade seed increased the germination rate.https://www.mdpi.com/2311-7524/10/12/1342sticky nightshadegerminationgibberellinstarch granules
spellingShingle Haobo Xu
Danni Mo
Xingyu Zhang
Fangman Li
Jinbao Tao
Pingfei Ge
Yang Yang
Ziyuan Wang
Yuyang Zhang
Gibberellin Treatment Accelerates Starch Decomposition and Seed Germination in Sticky Nightshade (<i>Solanum sisymbriifolium</i> Lam.)
Horticulturae
sticky nightshade
germination
gibberellin
starch granules
title Gibberellin Treatment Accelerates Starch Decomposition and Seed Germination in Sticky Nightshade (<i>Solanum sisymbriifolium</i> Lam.)
title_full Gibberellin Treatment Accelerates Starch Decomposition and Seed Germination in Sticky Nightshade (<i>Solanum sisymbriifolium</i> Lam.)
title_fullStr Gibberellin Treatment Accelerates Starch Decomposition and Seed Germination in Sticky Nightshade (<i>Solanum sisymbriifolium</i> Lam.)
title_full_unstemmed Gibberellin Treatment Accelerates Starch Decomposition and Seed Germination in Sticky Nightshade (<i>Solanum sisymbriifolium</i> Lam.)
title_short Gibberellin Treatment Accelerates Starch Decomposition and Seed Germination in Sticky Nightshade (<i>Solanum sisymbriifolium</i> Lam.)
title_sort gibberellin treatment accelerates starch decomposition and seed germination in sticky nightshade i solanum sisymbriifolium i lam
topic sticky nightshade
germination
gibberellin
starch granules
url https://www.mdpi.com/2311-7524/10/12/1342
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