Semi-rational evolution of a recombinant DNA polymerase for modified nucleotide incorporation efficiency.

Semi-rational evolution of a recombinant DNA polymerase for modified nucleotide incorporation efficiency.

Engineering improved B-family DNA polymerases to catalyze 3'-O-modified nucleotide reversible terminators is limited by an insufficient understanding of the structural determinants that define polymerization efficiency. To explore the key mechanism for unnatural nucleotide incorporation, we eng...

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Bibliographic Details
Main Authors: Lili Zhai, Zi Wang, Fen Liu, Chongjun Xu, Jingjing Wang, Hongyan Han, Qingqing Xie, Wenwei Zhang, Yue Zheng, Alexander K Buell, Yuliang Dong
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2025-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0316531
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