Transcriptome-Based Revelation of the Effects of Sleep Deprivation on Hepatic Metabolic Rhythms in Tibetan Sheep (<i>Ovis aries</i>)
Sleep deprivation (SD) disrupts circadian rhythms; however, its effects on SD and the mechanisms involved require further investigation. Previous studies on SD were mainly conducted on rodents, such as mice, with few studies on its effects on the liver of large diurnal animals, such as sheep. In thi...
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2024-11-01
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| author | Ya-Le Chen Ru Wang Rui Pang Zhi-Peng Sun Xiao-Long He Wen-Hui Tang Jing-Yu Ou Huan-Ming Yi Xiao Cheng Jia-Hong Chen Yang Yu Chun-Huan Ren Qiang-Jun Wang Zi-Jun Zhang |
| author_facet | Ya-Le Chen Ru Wang Rui Pang Zhi-Peng Sun Xiao-Long He Wen-Hui Tang Jing-Yu Ou Huan-Ming Yi Xiao Cheng Jia-Hong Chen Yang Yu Chun-Huan Ren Qiang-Jun Wang Zi-Jun Zhang |
| author_sort | Ya-Le Chen |
| collection | DOAJ |
| description | Sleep deprivation (SD) disrupts circadian rhythms; however, its effects on SD and the mechanisms involved require further investigation. Previous studies on SD were mainly conducted on rodents, such as mice, with few studies on its effects on the liver of large diurnal animals, such as sheep. In this study, we used a Tibetan sheep model for the first time to investigate the effects of SD on the liver by exposing Tibetan sheep (<i>Ovis aries</i>) to 7 days of SD (6 h/day) and performed transcriptome sequencing analysis on liver samples taken at 4 h intervals over 24 h. The results revealed that SD significantly altered the circadian expression of genes and their expression patterns in the liver of Tibetan sheep. Enrichment analysis of the circadian rhythm-altered genes revealed changes in the pathways related to lipid metabolism in the liver. Further evidence from serum markers and gene expression analyses using qualitative real-time polymerase chain reaction and Oil Red O and apoptosis staining indicated that SD leads to abnormal lipid metabolism in the liver, potentially causing liver damage. Therefore, our results suggest that SD disrupts the circadian rhythms of metabolism-related genes in the Tibetan sheep liver, thereby affecting metabolic homeostasis. |
| format | Article |
| id | doaj-art-50f0fd29e0714b48b4bf2768fb81469f |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj-art-50f0fd29e0714b48b4bf2768fb81469f2024-11-26T17:45:33ZengMDPI AGAnimals2076-26152024-11-011422316510.3390/ani14223165Transcriptome-Based Revelation of the Effects of Sleep Deprivation on Hepatic Metabolic Rhythms in Tibetan Sheep (<i>Ovis aries</i>)Ya-Le Chen0Ru Wang1Rui Pang2Zhi-Peng Sun3Xiao-Long He4Wen-Hui Tang5Jing-Yu Ou6Huan-Ming Yi7Xiao Cheng8Jia-Hong Chen9Yang Yu10Chun-Huan Ren11Qiang-Jun Wang12Zi-Jun Zhang13College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaChongqing Key Laboratory of Herbivore Science, College of Animal Science and Technology, Southwest University, Chongqing 400715, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCenter of Agriculture Technology Cooperation and Promotion of Dingyuan County, Chuzhou 233200, ChinaQinghai Provincial Key Laboratory of Adaptive Management on Alpine Grassland, Qinghai Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining 810016, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaSleep deprivation (SD) disrupts circadian rhythms; however, its effects on SD and the mechanisms involved require further investigation. Previous studies on SD were mainly conducted on rodents, such as mice, with few studies on its effects on the liver of large diurnal animals, such as sheep. In this study, we used a Tibetan sheep model for the first time to investigate the effects of SD on the liver by exposing Tibetan sheep (<i>Ovis aries</i>) to 7 days of SD (6 h/day) and performed transcriptome sequencing analysis on liver samples taken at 4 h intervals over 24 h. The results revealed that SD significantly altered the circadian expression of genes and their expression patterns in the liver of Tibetan sheep. Enrichment analysis of the circadian rhythm-altered genes revealed changes in the pathways related to lipid metabolism in the liver. Further evidence from serum markers and gene expression analyses using qualitative real-time polymerase chain reaction and Oil Red O and apoptosis staining indicated that SD leads to abnormal lipid metabolism in the liver, potentially causing liver damage. Therefore, our results suggest that SD disrupts the circadian rhythms of metabolism-related genes in the Tibetan sheep liver, thereby affecting metabolic homeostasis.https://www.mdpi.com/2076-2615/14/22/3165sleep deprivationcircadian rhythmliverlipid metabolismTibetan sheep |
| spellingShingle | Ya-Le Chen Ru Wang Rui Pang Zhi-Peng Sun Xiao-Long He Wen-Hui Tang Jing-Yu Ou Huan-Ming Yi Xiao Cheng Jia-Hong Chen Yang Yu Chun-Huan Ren Qiang-Jun Wang Zi-Jun Zhang Transcriptome-Based Revelation of the Effects of Sleep Deprivation on Hepatic Metabolic Rhythms in Tibetan Sheep (<i>Ovis aries</i>) Animals sleep deprivation circadian rhythm liver lipid metabolism Tibetan sheep |
| title | Transcriptome-Based Revelation of the Effects of Sleep Deprivation on Hepatic Metabolic Rhythms in Tibetan Sheep (<i>Ovis aries</i>) |
| title_full | Transcriptome-Based Revelation of the Effects of Sleep Deprivation on Hepatic Metabolic Rhythms in Tibetan Sheep (<i>Ovis aries</i>) |
| title_fullStr | Transcriptome-Based Revelation of the Effects of Sleep Deprivation on Hepatic Metabolic Rhythms in Tibetan Sheep (<i>Ovis aries</i>) |
| title_full_unstemmed | Transcriptome-Based Revelation of the Effects of Sleep Deprivation on Hepatic Metabolic Rhythms in Tibetan Sheep (<i>Ovis aries</i>) |
| title_short | Transcriptome-Based Revelation of the Effects of Sleep Deprivation on Hepatic Metabolic Rhythms in Tibetan Sheep (<i>Ovis aries</i>) |
| title_sort | transcriptome based revelation of the effects of sleep deprivation on hepatic metabolic rhythms in tibetan sheep i ovis aries i |
| topic | sleep deprivation circadian rhythm liver lipid metabolism Tibetan sheep |
| url | https://www.mdpi.com/2076-2615/14/22/3165 |
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