Dynamic Steerable Patterning of Microscale Particles and Living Cells Using an Ultrasound‐Phased Array
Acoustic patterning is a noncontact method to manipulate the spatial distribution of small particles using the forces generated in an ultrasound standing wave field. The technique has found applications in fields such as cell sorting, microfabrication, and tissue engineering. For tissue engineering,...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2025-04-01
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| Series: | Advanced NanoBiomed Research |
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| Online Access: | https://doi.org/10.1002/anbr.202400172 |
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| author | Rick J. P. van Bergen Bart G. W. Groenen Daniëlle C. A. Duffhues Richard G. P. Lopata Carlijn V. C. Bouten Hans‐Martin Schwab |
| author_facet | Rick J. P. van Bergen Bart G. W. Groenen Daniëlle C. A. Duffhues Richard G. P. Lopata Carlijn V. C. Bouten Hans‐Martin Schwab |
| author_sort | Rick J. P. van Bergen |
| collection | DOAJ |
| description | Acoustic patterning is a noncontact method to manipulate the spatial distribution of small particles using the forces generated in an ultrasound standing wave field. The technique has found applications in fields such as cell sorting, microfabrication, and tissue engineering. For tissue engineering, acoustic patterning enables remote cell and tissue manipulation, even in clinical settings. Conventional axial patterning strategies rely on reflector‐based or dual‐probe approaches, limiting their application to controlled setups incompatible with in vivo conditions. In contrast, single‐sided lateral patterning approaches, exploiting the transmit beamforming capabilities and tunability of a clinical ultrasound transducer array, can bridge the gap to in vivo applications. For the first time, a clinical‐phased array is used to acoustically pattern microscale particles in both axial and lateral directions, with dynamic control over pattern shape and orientation by adjusting electronic transducer delays. The data are used to validate a numerical model designed to predict acoustic forces and particle displacement in current and future experiments. Finally, acoustic patterning is successfully applied to living cells, demonstrating the potential translation of the proof of concept toward living tissues. In conclusion, clinical transducer arrays can pattern particles and living cells, augmenting patterning flexibility and advancing acoustic patterning for tissue engineering. |
| format | Article |
| id | doaj-art-50ea6846ee514a43b9767ce7ab9101b1 |
| institution | OA Journals |
| issn | 2699-9307 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced NanoBiomed Research |
| spelling | doaj-art-50ea6846ee514a43b9767ce7ab9101b12025-08-20T02:25:35ZengWiley-VCHAdvanced NanoBiomed Research2699-93072025-04-0154n/an/a10.1002/anbr.202400172Dynamic Steerable Patterning of Microscale Particles and Living Cells Using an Ultrasound‐Phased ArrayRick J. P. van Bergen0Bart G. W. Groenen1Daniëlle C. A. Duffhues2Richard G. P. Lopata3Carlijn V. C. Bouten4Hans‐Martin Schwab5Department of Biomedical Engineering Eindhoven University of Technology 5600 MB Eindhoven NetherlandsDepartment of Biomedical Engineering Eindhoven University of Technology 5600 MB Eindhoven NetherlandsDepartment of Biomedical Engineering Eindhoven University of Technology 5600 MB Eindhoven NetherlandsDepartment of Biomedical Engineering Eindhoven University of Technology 5600 MB Eindhoven NetherlandsDepartment of Biomedical Engineering Eindhoven University of Technology 5600 MB Eindhoven NetherlandsDepartment of Biomedical Engineering Eindhoven University of Technology 5600 MB Eindhoven NetherlandsAcoustic patterning is a noncontact method to manipulate the spatial distribution of small particles using the forces generated in an ultrasound standing wave field. The technique has found applications in fields such as cell sorting, microfabrication, and tissue engineering. For tissue engineering, acoustic patterning enables remote cell and tissue manipulation, even in clinical settings. Conventional axial patterning strategies rely on reflector‐based or dual‐probe approaches, limiting their application to controlled setups incompatible with in vivo conditions. In contrast, single‐sided lateral patterning approaches, exploiting the transmit beamforming capabilities and tunability of a clinical ultrasound transducer array, can bridge the gap to in vivo applications. For the first time, a clinical‐phased array is used to acoustically pattern microscale particles in both axial and lateral directions, with dynamic control over pattern shape and orientation by adjusting electronic transducer delays. The data are used to validate a numerical model designed to predict acoustic forces and particle displacement in current and future experiments. Finally, acoustic patterning is successfully applied to living cells, demonstrating the potential translation of the proof of concept toward living tissues. In conclusion, clinical transducer arrays can pattern particles and living cells, augmenting patterning flexibility and advancing acoustic patterning for tissue engineering.https://doi.org/10.1002/anbr.202400172acoustic patterningparticle manipulationtissue engineeringultrasound transducer arrays |
| spellingShingle | Rick J. P. van Bergen Bart G. W. Groenen Daniëlle C. A. Duffhues Richard G. P. Lopata Carlijn V. C. Bouten Hans‐Martin Schwab Dynamic Steerable Patterning of Microscale Particles and Living Cells Using an Ultrasound‐Phased Array Advanced NanoBiomed Research acoustic patterning particle manipulation tissue engineering ultrasound transducer arrays |
| title | Dynamic Steerable Patterning of Microscale Particles and Living Cells Using an Ultrasound‐Phased Array |
| title_full | Dynamic Steerable Patterning of Microscale Particles and Living Cells Using an Ultrasound‐Phased Array |
| title_fullStr | Dynamic Steerable Patterning of Microscale Particles and Living Cells Using an Ultrasound‐Phased Array |
| title_full_unstemmed | Dynamic Steerable Patterning of Microscale Particles and Living Cells Using an Ultrasound‐Phased Array |
| title_short | Dynamic Steerable Patterning of Microscale Particles and Living Cells Using an Ultrasound‐Phased Array |
| title_sort | dynamic steerable patterning of microscale particles and living cells using an ultrasound phased array |
| topic | acoustic patterning particle manipulation tissue engineering ultrasound transducer arrays |
| url | https://doi.org/10.1002/anbr.202400172 |
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