Current Perspectives in Detection of Bioactive Compounds From Costus spicatus Through GC–MS and LC-MS/MS: Antioxidant Properties and In Silico Analysis for Industrial Applications
Members of the Costus genus are the conventional medicinal plants used in the therapeutic management of numerous ailments, especially for their antioxidant and pharmacological activities. The crude extract of Costus spicatus was profiled using high-resolution GC–MS and LC-MS/MS techniques to determi...
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| Language: | English |
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Wiley
2025-01-01
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| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/bri/9599942 |
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| author | Dharmar Manimaran Ararsa Tessema M. Govindarajulu Yadav S. Parthasarathi Vasan Palanisamy |
| author_facet | Dharmar Manimaran Ararsa Tessema M. Govindarajulu Yadav S. Parthasarathi Vasan Palanisamy |
| author_sort | Dharmar Manimaran |
| collection | DOAJ |
| description | Members of the Costus genus are the conventional medicinal plants used in the therapeutic management of numerous ailments, especially for their antioxidant and pharmacological activities. The crude extract of Costus spicatus was profiled using high-resolution GC–MS and LC-MS/MS techniques to determine possible bioactive compounds that are vital to the antioxidant activity. A total of 52 and 63 bioactive compounds have been detected in GC–MS chromatograms using different solvents (methanol and ethanol) in C. spicatus leaf extracts, representing the presence of certain bioactive compounds. The identified bioactive compounds in both extracts which exhibit neuroprotective effects have been confirmed through various literature studies. They are cholestan-3-amine, loliolide, stigmasterol and methylprednisolone acetate, succinimide, fumaric acid, beta-tocopherol and gamma-tocopherol. The aqueous extract possessed the highest antioxidant activity for DPPH scavenging activity and lipid peroxidation inhibition assays, whereas the alcoholic aqueous extract showed superior efficacy for hydroxyl radical scavenging activity. In this study, we performed molecular docking and found that four compounds exhibited promising binding affinities with predicted binding sites on alpha-synuclein. Notably, Androsta [17-16-b] furan-5′-imine, 4′-methylene-3-methoxy-N-cyclohexyl- showed the highest docking interaction score of −7.4, indicating a strong binding affinity. These findings, combined with the presence of bioactive components in the crude extract of C. spicatus, suggest that this plant may possess neuroprotective properties, warranting further investigation for potential industrial applications in the development of neuroprotective agents. |
| format | Article |
| id | doaj-art-50ea02bf5c4741a0a767e2c87fce3b7b |
| institution | Kabale University |
| issn | 2090-2255 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-50ea02bf5c4741a0a767e2c87fce3b7b2025-01-23T05:00:03ZengWileyBiochemistry Research International2090-22552025-01-01202510.1155/bri/9599942Current Perspectives in Detection of Bioactive Compounds From Costus spicatus Through GC–MS and LC-MS/MS: Antioxidant Properties and In Silico Analysis for Industrial ApplicationsDharmar Manimaran0Ararsa Tessema1M. Govindarajulu Yadav2S. Parthasarathi3Vasan Palanisamy4Department of Animal NutritionDepartment of Food EngineeringDepartment of Food EngineeringDepartment of BiotechnologyDepartment of Animal NutritionMembers of the Costus genus are the conventional medicinal plants used in the therapeutic management of numerous ailments, especially for their antioxidant and pharmacological activities. The crude extract of Costus spicatus was profiled using high-resolution GC–MS and LC-MS/MS techniques to determine possible bioactive compounds that are vital to the antioxidant activity. A total of 52 and 63 bioactive compounds have been detected in GC–MS chromatograms using different solvents (methanol and ethanol) in C. spicatus leaf extracts, representing the presence of certain bioactive compounds. The identified bioactive compounds in both extracts which exhibit neuroprotective effects have been confirmed through various literature studies. They are cholestan-3-amine, loliolide, stigmasterol and methylprednisolone acetate, succinimide, fumaric acid, beta-tocopherol and gamma-tocopherol. The aqueous extract possessed the highest antioxidant activity for DPPH scavenging activity and lipid peroxidation inhibition assays, whereas the alcoholic aqueous extract showed superior efficacy for hydroxyl radical scavenging activity. In this study, we performed molecular docking and found that four compounds exhibited promising binding affinities with predicted binding sites on alpha-synuclein. Notably, Androsta [17-16-b] furan-5′-imine, 4′-methylene-3-methoxy-N-cyclohexyl- showed the highest docking interaction score of −7.4, indicating a strong binding affinity. These findings, combined with the presence of bioactive components in the crude extract of C. spicatus, suggest that this plant may possess neuroprotective properties, warranting further investigation for potential industrial applications in the development of neuroprotective agents.http://dx.doi.org/10.1155/bri/9599942 |
| spellingShingle | Dharmar Manimaran Ararsa Tessema M. Govindarajulu Yadav S. Parthasarathi Vasan Palanisamy Current Perspectives in Detection of Bioactive Compounds From Costus spicatus Through GC–MS and LC-MS/MS: Antioxidant Properties and In Silico Analysis for Industrial Applications Biochemistry Research International |
| title | Current Perspectives in Detection of Bioactive Compounds From Costus spicatus Through GC–MS and LC-MS/MS: Antioxidant Properties and In Silico Analysis for Industrial Applications |
| title_full | Current Perspectives in Detection of Bioactive Compounds From Costus spicatus Through GC–MS and LC-MS/MS: Antioxidant Properties and In Silico Analysis for Industrial Applications |
| title_fullStr | Current Perspectives in Detection of Bioactive Compounds From Costus spicatus Through GC–MS and LC-MS/MS: Antioxidant Properties and In Silico Analysis for Industrial Applications |
| title_full_unstemmed | Current Perspectives in Detection of Bioactive Compounds From Costus spicatus Through GC–MS and LC-MS/MS: Antioxidant Properties and In Silico Analysis for Industrial Applications |
| title_short | Current Perspectives in Detection of Bioactive Compounds From Costus spicatus Through GC–MS and LC-MS/MS: Antioxidant Properties and In Silico Analysis for Industrial Applications |
| title_sort | current perspectives in detection of bioactive compounds from costus spicatus through gc ms and lc ms ms antioxidant properties and in silico analysis for industrial applications |
| url | http://dx.doi.org/10.1155/bri/9599942 |
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