Positive Effect of Camelina Intercropping with Legumes on Soil Microbial Diversity by Applying NGS Analysis and Mobile Fluorescence Spectroscopy
Camelina (<i>Camelina sativa</i> (L.) Crantz) is a valuable source of essential amino acids, especially sulphur-containing ones, which are generally lacking in leguminous crops, thus representing an alternative source of protein for both humans and farm animals. Rhizosphere soil samples...
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2024-10-01
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| author | Marina Marcheva Mariana Petkova Vanya Slavova Vladislav Popov |
| author_facet | Marina Marcheva Mariana Petkova Vanya Slavova Vladislav Popov |
| author_sort | Marina Marcheva |
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| description | Camelina (<i>Camelina sativa</i> (L.) Crantz) is a valuable source of essential amino acids, especially sulphur-containing ones, which are generally lacking in leguminous crops, thus representing an alternative source of protein for both humans and farm animals. Rhizosphere soil samples from five experimental plots with mono- and mixed cultivations of three camelina cultivars, including two introduced varieties Cs1.Pro (Luna) and Cs2.Pro (Lenka) and one Bulgarian variety Cs3.Pro (local Bulgarian landrace) with variety 666 of vetch (<i>Vicia sativa</i> L.) (Cs3-Vs.Pro) and variety Mir of pea (<i>Pisum sativum</i> L.) (Cs3-Ps.Pro), were collected and analysed. The total DNA was isolated from the rhizosphere soils and the presence of the 16S rRNA gene was confirmed by amplification with the universal primer 16SV34. In the present study, the structure of the soil bacterial community in five different plots (Cs1.S.Pro, Cs2.S.Pro, Cs3.S.Pro, Cs3.Vs.S.Pro, and Cs3.Ps.S.Pro) where camelina was grown alone and by being intercropped with pea and vetch was analysed via a metagenomic approach. The number of observed species was highest in the local genotype of the camelina Cs3 grown alone, followed by soil from the intercropped variants Cs3-Vs and CsS-Ps. The soil bacterial communities differed between the sole cultivation of camelina and that grown with joint cultivation with vetch and peas, indicating that legumes considerably affected the growth and development of beneficial microorganisms by aspects such as nitrogen fixing, levels of nitrifying bacteria, and levels of phosphorus-dissolving bacteria, thus helping to provide better plant nutrition. The α-diversity indicated that bacterial communities in the rhizosphere were higher in soils intercropped with vetch and pea. The optical properties of cereals and legumes were determined by their energy structure, which includes both their occupied and free electronic energy levels and the energy levels of the atomic vibrations of the molecules or the crystal lattice. |
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| spelling | doaj-art-50e3f65d5ea6444d84d5c9a7266f2ea72025-08-20T01:47:42ZengMDPI AGApplied Sciences2076-34172024-10-011419904610.3390/app14199046Positive Effect of Camelina Intercropping with Legumes on Soil Microbial Diversity by Applying NGS Analysis and Mobile Fluorescence SpectroscopyMarina Marcheva0Mariana Petkova1Vanya Slavova2Vladislav Popov3Department of Crop Science, Faculty of Agronomy, Agricultural University Plovdiv, 12 Mendeleev blvd, 4000 Plovdiv, BulgariaDepartment of Microbiology and Environmental Biotechnology, Faculty of Plant Protection and Agroecology, Agricultural University Plovdiv, 12 Mendeleev blvd, 4000 Plovdiv, BulgariaMaritsa Vegetable Crops Research Institute, Agricultural Academy Bulgaria, 32 Brezovsko shosse St., 4003 Plovdiv, BulgariaDepartment of Agroecology, Faculty of Plant Protection and Agroecology, Agricultural University Plovdiv, 12 Mendeleev blvd, 4000 Plovdiv, BulgariaCamelina (<i>Camelina sativa</i> (L.) Crantz) is a valuable source of essential amino acids, especially sulphur-containing ones, which are generally lacking in leguminous crops, thus representing an alternative source of protein for both humans and farm animals. Rhizosphere soil samples from five experimental plots with mono- and mixed cultivations of three camelina cultivars, including two introduced varieties Cs1.Pro (Luna) and Cs2.Pro (Lenka) and one Bulgarian variety Cs3.Pro (local Bulgarian landrace) with variety 666 of vetch (<i>Vicia sativa</i> L.) (Cs3-Vs.Pro) and variety Mir of pea (<i>Pisum sativum</i> L.) (Cs3-Ps.Pro), were collected and analysed. The total DNA was isolated from the rhizosphere soils and the presence of the 16S rRNA gene was confirmed by amplification with the universal primer 16SV34. In the present study, the structure of the soil bacterial community in five different plots (Cs1.S.Pro, Cs2.S.Pro, Cs3.S.Pro, Cs3.Vs.S.Pro, and Cs3.Ps.S.Pro) where camelina was grown alone and by being intercropped with pea and vetch was analysed via a metagenomic approach. The number of observed species was highest in the local genotype of the camelina Cs3 grown alone, followed by soil from the intercropped variants Cs3-Vs and CsS-Ps. The soil bacterial communities differed between the sole cultivation of camelina and that grown with joint cultivation with vetch and peas, indicating that legumes considerably affected the growth and development of beneficial microorganisms by aspects such as nitrogen fixing, levels of nitrifying bacteria, and levels of phosphorus-dissolving bacteria, thus helping to provide better plant nutrition. The α-diversity indicated that bacterial communities in the rhizosphere were higher in soils intercropped with vetch and pea. The optical properties of cereals and legumes were determined by their energy structure, which includes both their occupied and free electronic energy levels and the energy levels of the atomic vibrations of the molecules or the crystal lattice.https://www.mdpi.com/2076-3417/14/19/9046camelina sativaintercropping with pea and vetchmobile fluorescence spectroscopyNGSsoil microbiome |
| spellingShingle | Marina Marcheva Mariana Petkova Vanya Slavova Vladislav Popov Positive Effect of Camelina Intercropping with Legumes on Soil Microbial Diversity by Applying NGS Analysis and Mobile Fluorescence Spectroscopy Applied Sciences camelina sativa intercropping with pea and vetch mobile fluorescence spectroscopy NGS soil microbiome |
| title | Positive Effect of Camelina Intercropping with Legumes on Soil Microbial Diversity by Applying NGS Analysis and Mobile Fluorescence Spectroscopy |
| title_full | Positive Effect of Camelina Intercropping with Legumes on Soil Microbial Diversity by Applying NGS Analysis and Mobile Fluorescence Spectroscopy |
| title_fullStr | Positive Effect of Camelina Intercropping with Legumes on Soil Microbial Diversity by Applying NGS Analysis and Mobile Fluorescence Spectroscopy |
| title_full_unstemmed | Positive Effect of Camelina Intercropping with Legumes on Soil Microbial Diversity by Applying NGS Analysis and Mobile Fluorescence Spectroscopy |
| title_short | Positive Effect of Camelina Intercropping with Legumes on Soil Microbial Diversity by Applying NGS Analysis and Mobile Fluorescence Spectroscopy |
| title_sort | positive effect of camelina intercropping with legumes on soil microbial diversity by applying ngs analysis and mobile fluorescence spectroscopy |
| topic | camelina sativa intercropping with pea and vetch mobile fluorescence spectroscopy NGS soil microbiome |
| url | https://www.mdpi.com/2076-3417/14/19/9046 |
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