A Self-Emulsifying Nanococktail of Pomegranate and Cannabidiol Reduces Cognitive Decline in Mice
Oded Shoseyov,1,2 Avi Palatnik,1 Maayan Dadon-Freiberg,1 Orit Dagan,1 Ronit Shaltiel Karyo1 1Tahiro Inc, Encino, CA, 91436, USA; 2The Robert H. Smith Institute of Plant Science and Genetics, The Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, IsraelCorrespo...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2025-02-01
|
| Series: | Nutrition and Dietary Supplements |
| Subjects: | |
| Online Access: | https://www.dovepress.com/a-self-emulsifying-nanococktail-of-pomegranate-and-cannabidiol-reduces-peer-reviewed-fulltext-article-NDS |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832540596091224064 |
|---|---|
| author | Shoseyov O Palatnik A Dadon-Freiberg M Dagan O Shaltiel Karyo R |
| author_facet | Shoseyov O Palatnik A Dadon-Freiberg M Dagan O Shaltiel Karyo R |
| author_sort | Shoseyov O |
| collection | DOAJ |
| description | Oded Shoseyov,1,2 Avi Palatnik,1 Maayan Dadon-Freiberg,1 Orit Dagan,1 Ronit Shaltiel Karyo1 1Tahiro Inc, Encino, CA, 91436, USA; 2The Robert H. Smith Institute of Plant Science and Genetics, The Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, IsraelCorrespondence: Ronit Shaltiel Karyo, Tahiro Inc, 16130 Ventura Blvd., Suite 570, Encino, CA, 91436, USA, Email ronitsh80@gmail.comIntroduction: Stress and inflammation in the early stages of cognitive decline has spurred interest in the use of antioxidants as prophylactic and therapeutic agents. Several studies have established the neuroprotective capacities of a pomegranate oil nano-formulation (Nano-PSO). In parallel, cannabis-derived cannabidiol (CBD) prevented social recognition deficits and reduced neuroinflammation in mice. This work assessed the impact of Nano-PSO:CBD on memory and learning.Methods: A battery of hemp extracts was screened for their effects on acetylcholine esterase inhibition and neuronal protection activities. The best-performing extract in the in-vitro assays was nano-formulated via self-emulsifying system with PSO and administered to aged mice and mice with MK-801-induced cognitive decline, which were then subjected to a Morris water maze test and Y-maze test, respectively.Results: Aged mice chronically fed Nano-PSO:CBD performed with a 22– 31% shorter escape latency (p ≤ 0.05), 23% shorter distance swam and increased average speed as compared to aged control mice. Mice pretreated with Nano-PSO:CBD prior to MK-801 had a significantly higher percentage of entries into the novel arm of the Y-maze compared to untreated controls.Discussion: PSO nano-formulated with CBD had a salutary effect on memory, learning and spatial recognition and should be further studied in a clinical context.Keywords: cognition, nanoformulation, CBD, pomegranate seed oil, nano-omega 5, brain protect, punicic acid |
| format | Article |
| id | doaj-art-50e395183c684b0f9a5da24ed2ad19df |
| institution | Kabale University |
| issn | 1179-1489 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Nutrition and Dietary Supplements |
| spelling | doaj-art-50e395183c684b0f9a5da24ed2ad19df2025-02-04T17:15:41ZengDove Medical PressNutrition and Dietary Supplements1179-14892025-02-01Volume 17192899855A Self-Emulsifying Nanococktail of Pomegranate and Cannabidiol Reduces Cognitive Decline in MiceShoseyov OPalatnik ADadon-Freiberg MDagan OShaltiel Karyo ROded Shoseyov,1,2 Avi Palatnik,1 Maayan Dadon-Freiberg,1 Orit Dagan,1 Ronit Shaltiel Karyo1 1Tahiro Inc, Encino, CA, 91436, USA; 2The Robert H. Smith Institute of Plant Science and Genetics, The Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, IsraelCorrespondence: Ronit Shaltiel Karyo, Tahiro Inc, 16130 Ventura Blvd., Suite 570, Encino, CA, 91436, USA, Email ronitsh80@gmail.comIntroduction: Stress and inflammation in the early stages of cognitive decline has spurred interest in the use of antioxidants as prophylactic and therapeutic agents. Several studies have established the neuroprotective capacities of a pomegranate oil nano-formulation (Nano-PSO). In parallel, cannabis-derived cannabidiol (CBD) prevented social recognition deficits and reduced neuroinflammation in mice. This work assessed the impact of Nano-PSO:CBD on memory and learning.Methods: A battery of hemp extracts was screened for their effects on acetylcholine esterase inhibition and neuronal protection activities. The best-performing extract in the in-vitro assays was nano-formulated via self-emulsifying system with PSO and administered to aged mice and mice with MK-801-induced cognitive decline, which were then subjected to a Morris water maze test and Y-maze test, respectively.Results: Aged mice chronically fed Nano-PSO:CBD performed with a 22– 31% shorter escape latency (p ≤ 0.05), 23% shorter distance swam and increased average speed as compared to aged control mice. Mice pretreated with Nano-PSO:CBD prior to MK-801 had a significantly higher percentage of entries into the novel arm of the Y-maze compared to untreated controls.Discussion: PSO nano-formulated with CBD had a salutary effect on memory, learning and spatial recognition and should be further studied in a clinical context.Keywords: cognition, nanoformulation, CBD, pomegranate seed oil, nano-omega 5, brain protect, punicic acidhttps://www.dovepress.com/a-self-emulsifying-nanococktail-of-pomegranate-and-cannabidiol-reduces-peer-reviewed-fulltext-article-NDScognitionnanoformulationcbdpomegranate seed oilnano-omega 5brain protectpunicic acid |
| spellingShingle | Shoseyov O Palatnik A Dadon-Freiberg M Dagan O Shaltiel Karyo R A Self-Emulsifying Nanococktail of Pomegranate and Cannabidiol Reduces Cognitive Decline in Mice Nutrition and Dietary Supplements cognition nanoformulation cbd pomegranate seed oil nano-omega 5 brain protect punicic acid |
| title | A Self-Emulsifying Nanococktail of Pomegranate and Cannabidiol Reduces Cognitive Decline in Mice |
| title_full | A Self-Emulsifying Nanococktail of Pomegranate and Cannabidiol Reduces Cognitive Decline in Mice |
| title_fullStr | A Self-Emulsifying Nanococktail of Pomegranate and Cannabidiol Reduces Cognitive Decline in Mice |
| title_full_unstemmed | A Self-Emulsifying Nanococktail of Pomegranate and Cannabidiol Reduces Cognitive Decline in Mice |
| title_short | A Self-Emulsifying Nanococktail of Pomegranate and Cannabidiol Reduces Cognitive Decline in Mice |
| title_sort | self emulsifying nanococktail of pomegranate and cannabidiol reduces cognitive decline in mice |
| topic | cognition nanoformulation cbd pomegranate seed oil nano-omega 5 brain protect punicic acid |
| url | https://www.dovepress.com/a-self-emulsifying-nanococktail-of-pomegranate-and-cannabidiol-reduces-peer-reviewed-fulltext-article-NDS |
| work_keys_str_mv | AT shoseyovo aselfemulsifyingnanococktailofpomegranateandcannabidiolreducescognitivedeclineinmice AT palatnika aselfemulsifyingnanococktailofpomegranateandcannabidiolreducescognitivedeclineinmice AT dadonfreibergm aselfemulsifyingnanococktailofpomegranateandcannabidiolreducescognitivedeclineinmice AT dagano aselfemulsifyingnanococktailofpomegranateandcannabidiolreducescognitivedeclineinmice AT shaltielkaryor aselfemulsifyingnanococktailofpomegranateandcannabidiolreducescognitivedeclineinmice AT shoseyovo selfemulsifyingnanococktailofpomegranateandcannabidiolreducescognitivedeclineinmice AT palatnika selfemulsifyingnanococktailofpomegranateandcannabidiolreducescognitivedeclineinmice AT dadonfreibergm selfemulsifyingnanococktailofpomegranateandcannabidiolreducescognitivedeclineinmice AT dagano selfemulsifyingnanococktailofpomegranateandcannabidiolreducescognitivedeclineinmice AT shaltielkaryor selfemulsifyingnanococktailofpomegranateandcannabidiolreducescognitivedeclineinmice |