Aquaporin-1-Mediated Effects of Low Level He-Ne Laser Irradiation on Human Erythrocytes

The role of membrane aquaporin-1 (APQ-1) in the photobiomodulation (PBM) on erythrocyte deformability will be studied in this paper with human dehydrated erythrocytes as echinocytic shape alterations lead to decreased cellular deformability. Human dehydrated erythrocytes were irradiated with low int...

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Main Authors: Gang-Yue Luo, Li Sun, Timon Cheng-Yi Liu
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:International Journal of Photoenergy
Online Access:http://dx.doi.org/10.1155/2012/275209
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author Gang-Yue Luo
Li Sun
Timon Cheng-Yi Liu
author_facet Gang-Yue Luo
Li Sun
Timon Cheng-Yi Liu
author_sort Gang-Yue Luo
collection DOAJ
description The role of membrane aquaporin-1 (APQ-1) in the photobiomodulation (PBM) on erythrocyte deformability will be studied in this paper with human dehydrated erythrocytes as echinocytic shape alterations lead to decreased cellular deformability. Human dehydrated erythrocytes were irradiated with low intensity He-Ne laser irradiation (LHNL) at 0.9, 1.8, 2.7, and 4.4 mW/cm2 for 5, 15, and 30 min, respectively, and APQ-1 inhibitor, 0.2 μmol/L HgCl2, was used to study the role of APQ-1 in mediating PBM with LHNL at 4.4 mW/cm2 for 5 min. Comprehensive morphological parameters of an intact cell such as contact area, perimeter, roundness and erythrocyte elongation index (EEI) were measured to characterize erythrocyte deformability with fast micro multi-channel spectrophotometer. It was observed that the dosage of LHNL improvement of the morphological parameters of dehydrated erythrocytes was morphological-parameter-dependent, but the Bunsen-Roscoe rule did not hold for roundness. The LHNL at 4.4 mW/cm2 for 5 min significantly improved the contact area (P<0.05) and EEI (P<0.05) of the dehydrated erythrocytes, but the improvement was significantly inhibited by 0.2 μmol/L HgCl2 (P<0.05). It was concluded that AQP-1 might mediate the effects of LHNL on erythrocyte deformability, which supports the membranotropic mechanism of PBM.
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spelling doaj-art-50df5f5a389641388ff090b7db29679a2025-02-03T07:25:08ZengWileyInternational Journal of Photoenergy1110-662X1687-529X2012-01-01201210.1155/2012/275209275209Aquaporin-1-Mediated Effects of Low Level He-Ne Laser Irradiation on Human ErythrocytesGang-Yue Luo0Li Sun1Timon Cheng-Yi Liu2Laboratory of Laser Sports Medicine, South China Normal University, Guangzhou 510631, ChinaMedical School, Jinan University, Guangzhou 510632, ChinaLaboratory of Laser Sports Medicine, South China Normal University, Guangzhou 510631, ChinaThe role of membrane aquaporin-1 (APQ-1) in the photobiomodulation (PBM) on erythrocyte deformability will be studied in this paper with human dehydrated erythrocytes as echinocytic shape alterations lead to decreased cellular deformability. Human dehydrated erythrocytes were irradiated with low intensity He-Ne laser irradiation (LHNL) at 0.9, 1.8, 2.7, and 4.4 mW/cm2 for 5, 15, and 30 min, respectively, and APQ-1 inhibitor, 0.2 μmol/L HgCl2, was used to study the role of APQ-1 in mediating PBM with LHNL at 4.4 mW/cm2 for 5 min. Comprehensive morphological parameters of an intact cell such as contact area, perimeter, roundness and erythrocyte elongation index (EEI) were measured to characterize erythrocyte deformability with fast micro multi-channel spectrophotometer. It was observed that the dosage of LHNL improvement of the morphological parameters of dehydrated erythrocytes was morphological-parameter-dependent, but the Bunsen-Roscoe rule did not hold for roundness. The LHNL at 4.4 mW/cm2 for 5 min significantly improved the contact area (P<0.05) and EEI (P<0.05) of the dehydrated erythrocytes, but the improvement was significantly inhibited by 0.2 μmol/L HgCl2 (P<0.05). It was concluded that AQP-1 might mediate the effects of LHNL on erythrocyte deformability, which supports the membranotropic mechanism of PBM.http://dx.doi.org/10.1155/2012/275209
spellingShingle Gang-Yue Luo
Li Sun
Timon Cheng-Yi Liu
Aquaporin-1-Mediated Effects of Low Level He-Ne Laser Irradiation on Human Erythrocytes
International Journal of Photoenergy
title Aquaporin-1-Mediated Effects of Low Level He-Ne Laser Irradiation on Human Erythrocytes
title_full Aquaporin-1-Mediated Effects of Low Level He-Ne Laser Irradiation on Human Erythrocytes
title_fullStr Aquaporin-1-Mediated Effects of Low Level He-Ne Laser Irradiation on Human Erythrocytes
title_full_unstemmed Aquaporin-1-Mediated Effects of Low Level He-Ne Laser Irradiation on Human Erythrocytes
title_short Aquaporin-1-Mediated Effects of Low Level He-Ne Laser Irradiation on Human Erythrocytes
title_sort aquaporin 1 mediated effects of low level he ne laser irradiation on human erythrocytes
url http://dx.doi.org/10.1155/2012/275209
work_keys_str_mv AT gangyueluo aquaporin1mediatedeffectsoflowlevelhenelaserirradiationonhumanerythrocytes
AT lisun aquaporin1mediatedeffectsoflowlevelhenelaserirradiationonhumanerythrocytes
AT timonchengyiliu aquaporin1mediatedeffectsoflowlevelhenelaserirradiationonhumanerythrocytes