Screening and identification of the mutants from two-row barley cultivar ZJU3 induced by ethyl methane sulfonate (EMS)

The seeds of two-row barley (Hordeum vulgare L.) cultivar ZJU3 were treated by chemical mutagen ethyl methane sulfonate (EMS) to develop a mutant library. In M<sub>2</sub> generation, biological and agronomic characters were examined, and the identified mutants were validated in M<sub...

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Main Authors: ZHANG Xiao-qin, XUE Da-wei, ZHOU Wei-hui, WU Fei-bo, ZHANG Guo-ping
Format: Article
Language:English
Published: Zhejiang University Press 2011-03-01
Series:浙江大学学报. 农业与生命科学版
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Online Access:https://www.academax.com/doi/10.3785/j.issn.1008-9209.2011.02.008
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author ZHANG Xiao-qin
XUE Da-wei
ZHOU Wei-hui
WU Fei-bo
ZHANG Guo-ping
author_facet ZHANG Xiao-qin
XUE Da-wei
ZHOU Wei-hui
WU Fei-bo
ZHANG Guo-ping
author_sort ZHANG Xiao-qin
collection DOAJ
description The seeds of two-row barley (Hordeum vulgare L.) cultivar ZJU3 were treated by chemical mutagen ethyl methane sulfonate (EMS) to develop a mutant library. In M<sub>2</sub> generation, biological and agronomic characters were examined, and the identified mutants were validated in M<sub>3</sub> generation. Totally 97 phenotype mutants, involved in seedling habits, maturity, leaf morphology, plant height, spike shape and spikelet fertility were obtained from 3 000 treated plants, with mutated frequency of 7.46%. The results indicate the feasibility of developing barley mutant population using EMS treatment and the discussion is done on forward genetics approach of gene function study using mutant population for barley genetic improvement.
format Article
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institution Kabale University
issn 1008-9209
2097-5155
language English
publishDate 2011-03-01
publisher Zhejiang University Press
record_format Article
series 浙江大学学报. 农业与生命科学版
spelling doaj-art-50df119de7e34c6cbd1ed35eac114bc52025-08-20T03:58:11ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552011-03-013716917410.3785/j.issn.1008-9209.2011.02.00810089209Screening and identification of the mutants from two-row barley cultivar ZJU3 induced by ethyl methane sulfonate (EMS)ZHANG Xiao-qinXUE Da-weiZHOU Wei-huiWU Fei-boZHANG Guo-pingThe seeds of two-row barley (Hordeum vulgare L.) cultivar ZJU3 were treated by chemical mutagen ethyl methane sulfonate (EMS) to develop a mutant library. In M<sub>2</sub> generation, biological and agronomic characters were examined, and the identified mutants were validated in M<sub>3</sub> generation. Totally 97 phenotype mutants, involved in seedling habits, maturity, leaf morphology, plant height, spike shape and spikelet fertility were obtained from 3 000 treated plants, with mutated frequency of 7.46%. The results indicate the feasibility of developing barley mutant population using EMS treatment and the discussion is done on forward genetics approach of gene function study using mutant population for barley genetic improvement.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2011.02.008barleyethyl methane sulfonate (EMS)mutantpopulation
spellingShingle ZHANG Xiao-qin
XUE Da-wei
ZHOU Wei-hui
WU Fei-bo
ZHANG Guo-ping
Screening and identification of the mutants from two-row barley cultivar ZJU3 induced by ethyl methane sulfonate (EMS)
浙江大学学报. 农业与生命科学版
barley
ethyl methane sulfonate (EMS)
mutant
population
title Screening and identification of the mutants from two-row barley cultivar ZJU3 induced by ethyl methane sulfonate (EMS)
title_full Screening and identification of the mutants from two-row barley cultivar ZJU3 induced by ethyl methane sulfonate (EMS)
title_fullStr Screening and identification of the mutants from two-row barley cultivar ZJU3 induced by ethyl methane sulfonate (EMS)
title_full_unstemmed Screening and identification of the mutants from two-row barley cultivar ZJU3 induced by ethyl methane sulfonate (EMS)
title_short Screening and identification of the mutants from two-row barley cultivar ZJU3 induced by ethyl methane sulfonate (EMS)
title_sort screening and identification of the mutants from two row barley cultivar zju3 induced by ethyl methane sulfonate ems
topic barley
ethyl methane sulfonate (EMS)
mutant
population
url https://www.academax.com/doi/10.3785/j.issn.1008-9209.2011.02.008
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