Screening and identification of the mutants from two-row barley cultivar ZJU3 induced by ethyl methane sulfonate (EMS)
The seeds of two-row barley (Hordeum vulgare L.) cultivar ZJU3 were treated by chemical mutagen ethyl methane sulfonate (EMS) to develop a mutant library. In M<sub>2</sub> generation, biological and agronomic characters were examined, and the identified mutants were validated in M<sub...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2011-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2011.02.008 |
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| Summary: | The seeds of two-row barley (Hordeum vulgare L.) cultivar ZJU3 were treated by chemical mutagen ethyl methane sulfonate (EMS) to develop a mutant library. In M<sub>2</sub> generation, biological and agronomic characters were examined, and the identified mutants were validated in M<sub>3</sub> generation. Totally 97 phenotype mutants, involved in seedling habits, maturity, leaf morphology, plant height, spike shape and spikelet fertility were obtained from 3 000 treated plants, with mutated frequency of 7.46%. The results indicate the feasibility of developing barley mutant population using EMS treatment and the discussion is done on forward genetics approach of gene function study using mutant population for barley genetic improvement. |
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| ISSN: | 1008-9209 2097-5155 |