Marrow Stromal Cell Infusion Rescues Hematopoiesis in Lethally Irradiated Mice despite Rapid Clearance after Infusion
Marrow stromal cells (MSCs, also termed mesenchymal stem cells) have been proposed as a promising cellular therapy for tissue injury including radiation-induced marrow failure, but evidence for a direct effect is lacking. To assess the effects of MSCs on survival after lethal irradiation, we infused...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Advances in Hematology |
| Online Access: | http://dx.doi.org/10.1155/2012/142530 |
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| author | Xiaodong Yang Ilango Balakrishnan Beverly Torok-Storb Manoj M. Pillai |
| author_facet | Xiaodong Yang Ilango Balakrishnan Beverly Torok-Storb Manoj M. Pillai |
| author_sort | Xiaodong Yang |
| collection | DOAJ |
| description | Marrow stromal cells (MSCs, also termed mesenchymal stem cells) have been proposed as a promising cellular therapy for tissue injury including radiation-induced marrow failure, but evidence for a direct effect is lacking. To assess the effects of MSCs on survival after lethal irradiation, we infused syngeneic MSCs (either as immortalized MSCs clones or primary MSCs) intravenously into wild-type C57/Bl6 mice within 24 hours of lethal total body irradiation (TBI). Mice receiving either of the MSC preparations had significantly improved survival when compared to controls. In vivo imaging, immune histochemistry, and RT-PCR employed to detect MSCs indicated that the infused MSCs were predominantly localized to the lungs and rapidly cleared following infusion. Our results suggest that a single infusion of MSCs can improve survival after otherwise lethal TBI but the effect is not due to a direct interaction with, or contribution to, the damaged marrow by MSCs. |
| format | Article |
| id | doaj-art-50d4559cf5c8465a8b58d1bd529d9caf |
| institution | OA Journals |
| issn | 1687-9104 1687-9112 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Hematology |
| spelling | doaj-art-50d4559cf5c8465a8b58d1bd529d9caf2025-08-20T02:05:41ZengWileyAdvances in Hematology1687-91041687-91122012-01-01201210.1155/2012/142530142530Marrow Stromal Cell Infusion Rescues Hematopoiesis in Lethally Irradiated Mice despite Rapid Clearance after InfusionXiaodong Yang0Ilango Balakrishnan1Beverly Torok-Storb2Manoj M. Pillai3Department of Medicine, University of Colorado Denver, Aurora, CO 80045, USADepartment of Medicine, University of Colorado Denver, Aurora, CO 80045, USAClinical Research Division, Fred Hutchinson Cancer Research Centre, Seattle, WA 98109, USADepartment of Medicine, University of Colorado Denver, Aurora, CO 80045, USAMarrow stromal cells (MSCs, also termed mesenchymal stem cells) have been proposed as a promising cellular therapy for tissue injury including radiation-induced marrow failure, but evidence for a direct effect is lacking. To assess the effects of MSCs on survival after lethal irradiation, we infused syngeneic MSCs (either as immortalized MSCs clones or primary MSCs) intravenously into wild-type C57/Bl6 mice within 24 hours of lethal total body irradiation (TBI). Mice receiving either of the MSC preparations had significantly improved survival when compared to controls. In vivo imaging, immune histochemistry, and RT-PCR employed to detect MSCs indicated that the infused MSCs were predominantly localized to the lungs and rapidly cleared following infusion. Our results suggest that a single infusion of MSCs can improve survival after otherwise lethal TBI but the effect is not due to a direct interaction with, or contribution to, the damaged marrow by MSCs.http://dx.doi.org/10.1155/2012/142530 |
| spellingShingle | Xiaodong Yang Ilango Balakrishnan Beverly Torok-Storb Manoj M. Pillai Marrow Stromal Cell Infusion Rescues Hematopoiesis in Lethally Irradiated Mice despite Rapid Clearance after Infusion Advances in Hematology |
| title | Marrow Stromal Cell Infusion Rescues Hematopoiesis in Lethally Irradiated Mice despite Rapid Clearance after Infusion |
| title_full | Marrow Stromal Cell Infusion Rescues Hematopoiesis in Lethally Irradiated Mice despite Rapid Clearance after Infusion |
| title_fullStr | Marrow Stromal Cell Infusion Rescues Hematopoiesis in Lethally Irradiated Mice despite Rapid Clearance after Infusion |
| title_full_unstemmed | Marrow Stromal Cell Infusion Rescues Hematopoiesis in Lethally Irradiated Mice despite Rapid Clearance after Infusion |
| title_short | Marrow Stromal Cell Infusion Rescues Hematopoiesis in Lethally Irradiated Mice despite Rapid Clearance after Infusion |
| title_sort | marrow stromal cell infusion rescues hematopoiesis in lethally irradiated mice despite rapid clearance after infusion |
| url | http://dx.doi.org/10.1155/2012/142530 |
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