Performance of the Abbott Real Time CT/NG assay in urines and cervico-vaginal samples from Senegal
Introduction: Chlamydia trachomatis and Neisseria gonorrhoeae are the most common causes of sexually transmitted disease in Senegal and worldwide. Molecular techniques have become the standard for their detection, and due to the frequency of co-infections, these tests can detect both agents and can...
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The Journal of Infection in Developing Countries
2014-07-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/4026 |
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| author | Sokhna B Gueye Halimatou Diop-Ndiaye Aliou Gningue Ousseynou Ndiaye Abdou S Mbengue Aïssatou Gaye-Diallo Angelique Ndjioyi Souleymane Mboup Coumba Touré-Kane |
| author_facet | Sokhna B Gueye Halimatou Diop-Ndiaye Aliou Gningue Ousseynou Ndiaye Abdou S Mbengue Aïssatou Gaye-Diallo Angelique Ndjioyi Souleymane Mboup Coumba Touré-Kane |
| author_sort | Sokhna B Gueye |
| collection | DOAJ |
| description | Introduction: Chlamydia trachomatis and Neisseria gonorrhoeae are the most common causes of sexually transmitted disease in Senegal and worldwide. Molecular techniques have become the standard for their detection, and due to the frequency of co-infections, these tests can detect both agents and can be used on urine samples, vaginal swabs, or endocervical samples. In developing countries, the use of these molecular techniques is very limited and there is a need for evaluations of these techniques to be done.
Methodology: A total of 181 samples were tested with the Abbott RealTime CT/NG assay and compared with the Roche Cobas Amplicor CT/NG assay. Specimens were collected from the key population of men having sex with men (urine, n = 60), female sex workers (genital swabs, n = 60) and from women visiting the laboratory for a gynecological checkup (urine, n = 60 and endocervical samples, n = 61).
Results: The agreement between the two techniques was 98.90% with a Kappa coefficient of 0.98. A sensitivity of 93.3%, a specificity of 100%, a positive predictive value of 100%, and a negative predictive value of 93.3% were found for both Chlamydia trachomatis and Neisseria gonorrhoeae.
Conclusion: These results showed that both methods are similar and suitable for the detection of CT/NG in all types of samples examined in this study.
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| format | Article |
| id | doaj-art-50d29db0779142a7b019492366ac308f |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2014-07-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-50d29db0779142a7b019492366ac308f2025-08-20T02:27:15ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802014-07-0180710.3855/jidc.4026Performance of the Abbott Real Time CT/NG assay in urines and cervico-vaginal samples from SenegalSokhna B Gueye0Halimatou Diop-Ndiaye1Aliou Gningue2Ousseynou Ndiaye3Abdou S Mbengue4Aïssatou Gaye-Diallo5Angelique Ndjioyi6Souleymane Mboup7Coumba Touré-Kane8Université Cheikh Anta Diop, Dakar, SenegalUniversité Cheikh Anta Diop, Dakar, SenegalUniversité Cheikh Anta Diop, Dakar, SenegalUniversité Cheikh Anta Diop, Dakar, SenegalUniversité Cheikh Anta Diop, Dakar, SenegalUniversité Cheikh Anta Diop, Dakar, SenegalUniversité des Sciences de la Santé, Libreville, GabonUniversité Cheikh Anta Diop, Dakar, SenegalUniversité Cheikh Anta Diop, Dakar, SenegalIntroduction: Chlamydia trachomatis and Neisseria gonorrhoeae are the most common causes of sexually transmitted disease in Senegal and worldwide. Molecular techniques have become the standard for their detection, and due to the frequency of co-infections, these tests can detect both agents and can be used on urine samples, vaginal swabs, or endocervical samples. In developing countries, the use of these molecular techniques is very limited and there is a need for evaluations of these techniques to be done. Methodology: A total of 181 samples were tested with the Abbott RealTime CT/NG assay and compared with the Roche Cobas Amplicor CT/NG assay. Specimens were collected from the key population of men having sex with men (urine, n = 60), female sex workers (genital swabs, n = 60) and from women visiting the laboratory for a gynecological checkup (urine, n = 60 and endocervical samples, n = 61). Results: The agreement between the two techniques was 98.90% with a Kappa coefficient of 0.98. A sensitivity of 93.3%, a specificity of 100%, a positive predictive value of 100%, and a negative predictive value of 93.3% were found for both Chlamydia trachomatis and Neisseria gonorrhoeae. Conclusion: These results showed that both methods are similar and suitable for the detection of CT/NG in all types of samples examined in this study. https://jidc.org/index.php/journal/article/view/4026Abbott RealTime CT/NG assayurinegenital swabs |
| spellingShingle | Sokhna B Gueye Halimatou Diop-Ndiaye Aliou Gningue Ousseynou Ndiaye Abdou S Mbengue Aïssatou Gaye-Diallo Angelique Ndjioyi Souleymane Mboup Coumba Touré-Kane Performance of the Abbott Real Time CT/NG assay in urines and cervico-vaginal samples from Senegal Journal of Infection in Developing Countries Abbott RealTime CT/NG assay urine genital swabs |
| title | Performance of the Abbott Real Time CT/NG assay in urines and cervico-vaginal samples from Senegal |
| title_full | Performance of the Abbott Real Time CT/NG assay in urines and cervico-vaginal samples from Senegal |
| title_fullStr | Performance of the Abbott Real Time CT/NG assay in urines and cervico-vaginal samples from Senegal |
| title_full_unstemmed | Performance of the Abbott Real Time CT/NG assay in urines and cervico-vaginal samples from Senegal |
| title_short | Performance of the Abbott Real Time CT/NG assay in urines and cervico-vaginal samples from Senegal |
| title_sort | performance of the abbott real time ct ng assay in urines and cervico vaginal samples from senegal |
| topic | Abbott RealTime CT/NG assay urine genital swabs |
| url | https://jidc.org/index.php/journal/article/view/4026 |
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