Porcine SCD1 Regulates Lipid Droplet Number via CLSTN3B in PK15 Cells
Fat deposition plays a key role in determining porcine meat quality traits, with lipid droplets serving as critical organelles for lipid storage in adipose tissue. Inhibiting lipid droplet biogenesis disrupts the lipid storage capacity of adipocytes. The Stearoyl-CoA Desaturase (<i>SCD</i&g...
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MDPI AG
2025-06-01
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| author | Wenzhe Bai Qianhai Fang Yanzhen Bi Rui Wang Ke Xu Ao Zhou Hao Gu Hongbo Chen |
| author_facet | Wenzhe Bai Qianhai Fang Yanzhen Bi Rui Wang Ke Xu Ao Zhou Hao Gu Hongbo Chen |
| author_sort | Wenzhe Bai |
| collection | DOAJ |
| description | Fat deposition plays a key role in determining porcine meat quality traits, with lipid droplets serving as critical organelles for lipid storage in adipose tissue. Inhibiting lipid droplet biogenesis disrupts the lipid storage capacity of adipocytes. The Stearoyl-CoA Desaturase (<i>SCD</i>) family is crucial in regulating polyunsaturated fatty acid/monounsaturated fatty acid (PUFA/MUFA) composition, while its role in lipid droplet formation remains unclear. This study employed CRISPR/Cas9 to create SCD1-deficient porcine renal epithelial cells (PK15), enabling an investigation into SCD1’s role in fatty acid composition and lipid droplet regulation. RNA-seq analysis was conducted to elucidate the mechanisms underlying SCD1’s impact on lipid droplet numbers. Results showed that SCD1 deletion significantly decreased triacylglycerols (TAG) content, altered fatty acid composition, and decreased lipid droplet numbers. Conversely, SCD1 overexpression increased lipid droplet numbers, confirming SCD1’s role in regulating lipid droplet abundance. RNA-seq analysis revealed that SCD1 regulates lipid metabolism via Calsyntenin 3β (<i>CLSTN3B</i>). Experimental validation confirmed the SCD1-CLSTN3B regulation of lipid droplet numbers. In summary, we discovered the role of SCD1 in regulating the number of lipid droplets, highlighting its potential impact on lipid metabolism and adipocyte function in pigs. |
| format | Article |
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| institution | DOAJ |
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| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-50c80f857dfd4db3a563bde5a366cc8c2025-08-20T03:10:54ZengMDPI AGAnimals2076-26152025-06-011511166310.3390/ani15111663Porcine SCD1 Regulates Lipid Droplet Number via CLSTN3B in PK15 CellsWenzhe Bai0Qianhai Fang1Yanzhen Bi2Rui Wang3Ke Xu4Ao Zhou5Hao Gu6Hongbo Chen7Laboratory of Genetic Breeding, Reproduction and Precision Livestock Farming, Hubei Provincial Center of Technology Innovation for Domestic Animal Breeding, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaKey Laboratory of Animal Embryo Engineering and Molecular Breeding of Hubei Province, Wuhan 430064, ChinaKey Laboratory of Animal Embryo Engineering and Molecular Breeding of Hubei Province, Wuhan 430064, ChinaLaboratory of Genetic Breeding, Reproduction and Precision Livestock Farming, Hubei Provincial Center of Technology Innovation for Domestic Animal Breeding, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaLaboratory of Genetic Breeding, Reproduction and Precision Livestock Farming, Hubei Provincial Center of Technology Innovation for Domestic Animal Breeding, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaLaboratory of Genetic Breeding, Reproduction and Precision Livestock Farming, Hubei Provincial Center of Technology Innovation for Domestic Animal Breeding, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaKey Laboratory of Animal Embryo Engineering and Molecular Breeding of Hubei Province, Wuhan 430064, ChinaLaboratory of Genetic Breeding, Reproduction and Precision Livestock Farming, Hubei Provincial Center of Technology Innovation for Domestic Animal Breeding, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaFat deposition plays a key role in determining porcine meat quality traits, with lipid droplets serving as critical organelles for lipid storage in adipose tissue. Inhibiting lipid droplet biogenesis disrupts the lipid storage capacity of adipocytes. The Stearoyl-CoA Desaturase (<i>SCD</i>) family is crucial in regulating polyunsaturated fatty acid/monounsaturated fatty acid (PUFA/MUFA) composition, while its role in lipid droplet formation remains unclear. This study employed CRISPR/Cas9 to create SCD1-deficient porcine renal epithelial cells (PK15), enabling an investigation into SCD1’s role in fatty acid composition and lipid droplet regulation. RNA-seq analysis was conducted to elucidate the mechanisms underlying SCD1’s impact on lipid droplet numbers. Results showed that SCD1 deletion significantly decreased triacylglycerols (TAG) content, altered fatty acid composition, and decreased lipid droplet numbers. Conversely, SCD1 overexpression increased lipid droplet numbers, confirming SCD1’s role in regulating lipid droplet abundance. RNA-seq analysis revealed that SCD1 regulates lipid metabolism via Calsyntenin 3β (<i>CLSTN3B</i>). Experimental validation confirmed the SCD1-CLSTN3B regulation of lipid droplet numbers. In summary, we discovered the role of SCD1 in regulating the number of lipid droplets, highlighting its potential impact on lipid metabolism and adipocyte function in pigs.https://www.mdpi.com/2076-2615/15/11/1663pig<i>SCD1</i><i>CLSTN3B</i>lipid dropletmeat quality |
| spellingShingle | Wenzhe Bai Qianhai Fang Yanzhen Bi Rui Wang Ke Xu Ao Zhou Hao Gu Hongbo Chen Porcine SCD1 Regulates Lipid Droplet Number via CLSTN3B in PK15 Cells Animals pig <i>SCD1</i> <i>CLSTN3B</i> lipid droplet meat quality |
| title | Porcine SCD1 Regulates Lipid Droplet Number via CLSTN3B in PK15 Cells |
| title_full | Porcine SCD1 Regulates Lipid Droplet Number via CLSTN3B in PK15 Cells |
| title_fullStr | Porcine SCD1 Regulates Lipid Droplet Number via CLSTN3B in PK15 Cells |
| title_full_unstemmed | Porcine SCD1 Regulates Lipid Droplet Number via CLSTN3B in PK15 Cells |
| title_short | Porcine SCD1 Regulates Lipid Droplet Number via CLSTN3B in PK15 Cells |
| title_sort | porcine scd1 regulates lipid droplet number via clstn3b in pk15 cells |
| topic | pig <i>SCD1</i> <i>CLSTN3B</i> lipid droplet meat quality |
| url | https://www.mdpi.com/2076-2615/15/11/1663 |
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