Exosomal MicroRNAs Derived from Human Amniotic Epithelial Cells Accelerate Wound Healing by Promoting the Proliferation and Migration of Fibroblasts
Previous work in our laboratory demonstrated that exosomes derived from human amniotic epithelial cells (hAECs) accelerated wound healing by promoting the proliferation and migration of fibroblasts. It is reported that exosomes, which are carriers of the microRNAs (miRNAs) and proteins, play an impo...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/5420463 |
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| author | Bin Zhao Xiaodong Li Xiaomin Shi Xueqin Shi Wei Zhang Gaofeng Wu Xujie Wang Linlin Su Dahai Hu |
| author_facet | Bin Zhao Xiaodong Li Xiaomin Shi Xueqin Shi Wei Zhang Gaofeng Wu Xujie Wang Linlin Su Dahai Hu |
| author_sort | Bin Zhao |
| collection | DOAJ |
| description | Previous work in our laboratory demonstrated that exosomes derived from human amniotic epithelial cells (hAECs) accelerated wound healing by promoting the proliferation and migration of fibroblasts. It is reported that exosomes, which are carriers of the microRNAs (miRNAs) and proteins, play an important role in the regulation of cell-to-cell communication. However, it is still unclear precisely which molecule or which group of molecules carried within hAEC-derived exosomes (hAEC-Exos) mediated wound healing. Here, we explored purified hAEC-Exos together with either proteinase K (PROse) or RNase A on the effect of fibroblasts and cutaneous wound healing. Our experiments demonstrated that hAEC-Exos were positive for exosomal markers CD9, CD63, and CD81. Also, we found that hAEC-Exos could be internalized by fibroblasts and then stimulated cell migration and proliferation. However, the promotive effect of hAEC-Exos was abolished by pretreating hAEC-Exos with RNase A, not PROse. Importantly, in vivo wound healing assay showed that local injection of hAEC-Exos or PROse pretreated hAEC-Exos at skin wounds significantly accelerated wound healing. Our findings revealed an important role of exosomal miRNAs in wound healing. |
| format | Article |
| id | doaj-art-50c42fe24fa64a0580b928a64cbbc218 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-50c42fe24fa64a0580b928a64cbbc2182025-02-03T06:14:13ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/54204635420463Exosomal MicroRNAs Derived from Human Amniotic Epithelial Cells Accelerate Wound Healing by Promoting the Proliferation and Migration of FibroblastsBin Zhao0Xiaodong Li1Xiaomin Shi2Xueqin Shi3Wei Zhang4Gaofeng Wu5Xujie Wang6Linlin Su7Dahai Hu8Department of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shannxi 710032, ChinaDepartment of Burns and Plastic Surgery, General Hospital of Lanzhou Petrochemical Company, Lanzhou, Gansu 730060, ChinaDepartment of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shannxi 710032, ChinaDepartment of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shannxi 710032, ChinaDepartment of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shannxi 710032, ChinaDepartment of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shannxi 710032, ChinaDepartment of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shannxi 710032, ChinaDepartment of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shannxi 710032, ChinaDepartment of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shannxi 710032, ChinaPrevious work in our laboratory demonstrated that exosomes derived from human amniotic epithelial cells (hAECs) accelerated wound healing by promoting the proliferation and migration of fibroblasts. It is reported that exosomes, which are carriers of the microRNAs (miRNAs) and proteins, play an important role in the regulation of cell-to-cell communication. However, it is still unclear precisely which molecule or which group of molecules carried within hAEC-derived exosomes (hAEC-Exos) mediated wound healing. Here, we explored purified hAEC-Exos together with either proteinase K (PROse) or RNase A on the effect of fibroblasts and cutaneous wound healing. Our experiments demonstrated that hAEC-Exos were positive for exosomal markers CD9, CD63, and CD81. Also, we found that hAEC-Exos could be internalized by fibroblasts and then stimulated cell migration and proliferation. However, the promotive effect of hAEC-Exos was abolished by pretreating hAEC-Exos with RNase A, not PROse. Importantly, in vivo wound healing assay showed that local injection of hAEC-Exos or PROse pretreated hAEC-Exos at skin wounds significantly accelerated wound healing. Our findings revealed an important role of exosomal miRNAs in wound healing.http://dx.doi.org/10.1155/2018/5420463 |
| spellingShingle | Bin Zhao Xiaodong Li Xiaomin Shi Xueqin Shi Wei Zhang Gaofeng Wu Xujie Wang Linlin Su Dahai Hu Exosomal MicroRNAs Derived from Human Amniotic Epithelial Cells Accelerate Wound Healing by Promoting the Proliferation and Migration of Fibroblasts Stem Cells International |
| title | Exosomal MicroRNAs Derived from Human Amniotic Epithelial Cells Accelerate Wound Healing by Promoting the Proliferation and Migration of Fibroblasts |
| title_full | Exosomal MicroRNAs Derived from Human Amniotic Epithelial Cells Accelerate Wound Healing by Promoting the Proliferation and Migration of Fibroblasts |
| title_fullStr | Exosomal MicroRNAs Derived from Human Amniotic Epithelial Cells Accelerate Wound Healing by Promoting the Proliferation and Migration of Fibroblasts |
| title_full_unstemmed | Exosomal MicroRNAs Derived from Human Amniotic Epithelial Cells Accelerate Wound Healing by Promoting the Proliferation and Migration of Fibroblasts |
| title_short | Exosomal MicroRNAs Derived from Human Amniotic Epithelial Cells Accelerate Wound Healing by Promoting the Proliferation and Migration of Fibroblasts |
| title_sort | exosomal micrornas derived from human amniotic epithelial cells accelerate wound healing by promoting the proliferation and migration of fibroblasts |
| url | http://dx.doi.org/10.1155/2018/5420463 |
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