The Effect of Explant Type and Growth Regulators on Callus Induction in Passiflora quadrangularis
The initiation of callus culture and the stabilization of cell suspensions are important steps in obtaining a callus mass for in vitro secondary metabolite production. The protocol described here could be used for efficient callus induction in a wide range of Passiflora spp. Murashige and Skoog medi...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
AcademicPres
2024-11-01
|
| Series: | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture |
| Subjects: | |
| Online Access: | https://journals.usamvcluj.ro/index.php/horticulture/article/view/14972 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850220000540884992 |
|---|---|
| author | Paula OROS Maria CANTOR Corina CĂTANĂ |
| author_facet | Paula OROS Maria CANTOR Corina CĂTANĂ |
| author_sort | Paula OROS |
| collection | DOAJ |
| description | The initiation of callus culture and the stabilization of cell suspensions are important steps in obtaining a callus mass for in vitro secondary metabolite production. The protocol described here could be used for efficient callus induction in a wide range of Passiflora spp. Murashige and Skoog medium supplemented with 2,4-Dichlorophenoxyacetic acid (2,4-D) and Picloram (PIC) in different concentrations and combinations with Benzylaminopurine (BAP) and Kinetin (KIN) were used for culture initiation. A total of 11 treatments and different plant organs as explant source were tested. Callus proliferation was performed in solid and liquid culture systems. The interaction of factors showed that internodal segments callused at 100% on medium containing 2 mg/l 2,4-D and 0.5 mg/l BAP. On this culture medium, the leaf fragments also performed the highest callusing rate (98.33%). For P. quadrangularis, the liquid culture system proved to be superior in terms of cell mass and dry matter content, which can be explained by the better access to nutrients and water in a liquid medium. This research demonstrates that P. quadrangularis callus culture is strongly influenced by the plant growth regulators combination, the type of explants and culture system. The cell suspension obtained is the first step in the secondary metabolite production system. |
| format | Article |
| id | doaj-art-50beb4a201da47eaae76f2a2011ebe56 |
| institution | OA Journals |
| issn | 1843-5254 1843-5394 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | AcademicPres |
| record_format | Article |
| series | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture |
| spelling | doaj-art-50beb4a201da47eaae76f2a2011ebe562025-08-20T02:07:12ZengAcademicPresBulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture1843-52541843-53942024-11-01812626910.15835/buasvmcn-hort:2024.003214972The Effect of Explant Type and Growth Regulators on Callus Induction in Passiflora quadrangularisPaula OROS0Maria CANTOR1Corina CĂTANĂ2Faculty of Horticulture and Business in Rural Development, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5 Mănăștur St., 400374, RomaniaFaculty of Horticulture and Business in Rural Development, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5 Mănăștur St., 400374, RomaniaFaculty of Horticulture and Business in Rural Development, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5 Mănăștur St., 400374, RomaniaThe initiation of callus culture and the stabilization of cell suspensions are important steps in obtaining a callus mass for in vitro secondary metabolite production. The protocol described here could be used for efficient callus induction in a wide range of Passiflora spp. Murashige and Skoog medium supplemented with 2,4-Dichlorophenoxyacetic acid (2,4-D) and Picloram (PIC) in different concentrations and combinations with Benzylaminopurine (BAP) and Kinetin (KIN) were used for culture initiation. A total of 11 treatments and different plant organs as explant source were tested. Callus proliferation was performed in solid and liquid culture systems. The interaction of factors showed that internodal segments callused at 100% on medium containing 2 mg/l 2,4-D and 0.5 mg/l BAP. On this culture medium, the leaf fragments also performed the highest callusing rate (98.33%). For P. quadrangularis, the liquid culture system proved to be superior in terms of cell mass and dry matter content, which can be explained by the better access to nutrients and water in a liquid medium. This research demonstrates that P. quadrangularis callus culture is strongly influenced by the plant growth regulators combination, the type of explants and culture system. The cell suspension obtained is the first step in the secondary metabolite production system.https://journals.usamvcluj.ro/index.php/horticulture/article/view/14972passion flower; callus; cell suspension,; secondary metabolite production. |
| spellingShingle | Paula OROS Maria CANTOR Corina CĂTANĂ The Effect of Explant Type and Growth Regulators on Callus Induction in Passiflora quadrangularis Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture passion flower; callus; cell suspension,; secondary metabolite production. |
| title | The Effect of Explant Type and Growth Regulators on Callus Induction in Passiflora quadrangularis |
| title_full | The Effect of Explant Type and Growth Regulators on Callus Induction in Passiflora quadrangularis |
| title_fullStr | The Effect of Explant Type and Growth Regulators on Callus Induction in Passiflora quadrangularis |
| title_full_unstemmed | The Effect of Explant Type and Growth Regulators on Callus Induction in Passiflora quadrangularis |
| title_short | The Effect of Explant Type and Growth Regulators on Callus Induction in Passiflora quadrangularis |
| title_sort | effect of explant type and growth regulators on callus induction in passiflora quadrangularis |
| topic | passion flower; callus; cell suspension,; secondary metabolite production. |
| url | https://journals.usamvcluj.ro/index.php/horticulture/article/view/14972 |
| work_keys_str_mv | AT paulaoros theeffectofexplanttypeandgrowthregulatorsoncallusinductioninpassifloraquadrangularis AT mariacantor theeffectofexplanttypeandgrowthregulatorsoncallusinductioninpassifloraquadrangularis AT corinacatana theeffectofexplanttypeandgrowthregulatorsoncallusinductioninpassifloraquadrangularis AT paulaoros effectofexplanttypeandgrowthregulatorsoncallusinductioninpassifloraquadrangularis AT mariacantor effectofexplanttypeandgrowthregulatorsoncallusinductioninpassifloraquadrangularis AT corinacatana effectofexplanttypeandgrowthregulatorsoncallusinductioninpassifloraquadrangularis |