Arsenic compounds activate MAPK and inhibit Akt pathways to induce apoptosis in MA‐10 mouse Leydig tumor cells
Abstract Arsenic compounds have been applied treating acute promyelocytic 1eukemia and solid tumors with brief mechanism investigations. In fact, we have demonstrated that sodium arsenite plus dimethylarsenic acid could activate apoptosis in MA‐10 mouse Leydig tumor cells by inducing caspase pathway...
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| Format: | Article |
| Language: | English |
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Wiley
2023-02-01
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| Series: | Cancer Medicine |
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| Online Access: | https://doi.org/10.1002/cam4.5068 |
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| author | Wei‐Sheng Juan Yi‐Fen Mu Chia‐Yih Wang Edmund‐Cheung So Yi‐Ping Lee Sheng‐Che Lin Bu‐Miin Huang |
| author_facet | Wei‐Sheng Juan Yi‐Fen Mu Chia‐Yih Wang Edmund‐Cheung So Yi‐Ping Lee Sheng‐Che Lin Bu‐Miin Huang |
| author_sort | Wei‐Sheng Juan |
| collection | DOAJ |
| description | Abstract Arsenic compounds have been applied treating acute promyelocytic 1eukemia and solid tumors with brief mechanism investigations. In fact, we have demonstrated that sodium arsenite plus dimethylarsenic acid could activate apoptosis in MA‐10 mouse Leydig tumor cells by inducing caspase pathways. However, detail underlying mechanisms how caspase cascade is regulated remains elusive. Therefore, the apoptotic mechanism of sodium arsenite plus dimethylarsenic acid were examined in MA‐10 cells in this study. Our results reveal that Fas/FasL protein expressions were stimulated by sodium arsenite plus dimethylarsenic acid in MA‐10 cells. In addition, reactive oxygen species (ROS) generation, cytochrome C release, Bid truncation, and Bax translocation were induced in MA‐10 cells by arsenic compounds. Moreover, activation of p38, JNK and ERK1/2, MAPK pathways was stimulated while Akt phosphorylated levels and Akt expression were decreased by sodium arsenite plus dimethylarsenic in MA‐10 cells. In conclusion, sodium arsenite and dimethylarsenic acid did activate MAPK pathway plus ROS generation, but suppress Akt pathway, to modulate caspase pathway and then induce MA‐10 cell apoptosis. |
| format | Article |
| id | doaj-art-50bcb6f030d241d2855a66a19578d65d |
| institution | OA Journals |
| issn | 2045-7634 |
| language | English |
| publishDate | 2023-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj-art-50bcb6f030d241d2855a66a19578d65d2025-08-20T01:53:22ZengWileyCancer Medicine2045-76342023-02-011233260327510.1002/cam4.5068Arsenic compounds activate MAPK and inhibit Akt pathways to induce apoptosis in MA‐10 mouse Leydig tumor cellsWei‐Sheng Juan0Yi‐Fen Mu1Chia‐Yih Wang2Edmund‐Cheung So3Yi‐Ping Lee4Sheng‐Che Lin5Bu‐Miin Huang6Department of Neurosurgery, An Nan Hospital China Medical University Tainan City TaiwanDepartment of Cell Biology and Anatomy, College of Medicine National Cheng Kung University Tainan TaiwanDepartment of Cell Biology and Anatomy, College of Medicine National Cheng Kung University Tainan TaiwanDepartment of Anesthesia & Medical Research, An Nan Hospital China Medical University Tainan City TaiwanDepartment of Cell Biology and Anatomy, College of Medicine National Cheng Kung University Tainan TaiwanDepartment of Plastic Surgical, An Nan Hospital China Medical University Tainan City TaiwanDepartment of Cell Biology and Anatomy, College of Medicine National Cheng Kung University Tainan TaiwanAbstract Arsenic compounds have been applied treating acute promyelocytic 1eukemia and solid tumors with brief mechanism investigations. In fact, we have demonstrated that sodium arsenite plus dimethylarsenic acid could activate apoptosis in MA‐10 mouse Leydig tumor cells by inducing caspase pathways. However, detail underlying mechanisms how caspase cascade is regulated remains elusive. Therefore, the apoptotic mechanism of sodium arsenite plus dimethylarsenic acid were examined in MA‐10 cells in this study. Our results reveal that Fas/FasL protein expressions were stimulated by sodium arsenite plus dimethylarsenic acid in MA‐10 cells. In addition, reactive oxygen species (ROS) generation, cytochrome C release, Bid truncation, and Bax translocation were induced in MA‐10 cells by arsenic compounds. Moreover, activation of p38, JNK and ERK1/2, MAPK pathways was stimulated while Akt phosphorylated levels and Akt expression were decreased by sodium arsenite plus dimethylarsenic in MA‐10 cells. In conclusion, sodium arsenite and dimethylarsenic acid did activate MAPK pathway plus ROS generation, but suppress Akt pathway, to modulate caspase pathway and then induce MA‐10 cell apoptosis.https://doi.org/10.1002/cam4.5068Aktdimethylarsenic acidLeydig tumor cellMAPKROSsodium arsenite |
| spellingShingle | Wei‐Sheng Juan Yi‐Fen Mu Chia‐Yih Wang Edmund‐Cheung So Yi‐Ping Lee Sheng‐Che Lin Bu‐Miin Huang Arsenic compounds activate MAPK and inhibit Akt pathways to induce apoptosis in MA‐10 mouse Leydig tumor cells Cancer Medicine Akt dimethylarsenic acid Leydig tumor cell MAPK ROS sodium arsenite |
| title | Arsenic compounds activate MAPK and inhibit Akt pathways to induce apoptosis in MA‐10 mouse Leydig tumor cells |
| title_full | Arsenic compounds activate MAPK and inhibit Akt pathways to induce apoptosis in MA‐10 mouse Leydig tumor cells |
| title_fullStr | Arsenic compounds activate MAPK and inhibit Akt pathways to induce apoptosis in MA‐10 mouse Leydig tumor cells |
| title_full_unstemmed | Arsenic compounds activate MAPK and inhibit Akt pathways to induce apoptosis in MA‐10 mouse Leydig tumor cells |
| title_short | Arsenic compounds activate MAPK and inhibit Akt pathways to induce apoptosis in MA‐10 mouse Leydig tumor cells |
| title_sort | arsenic compounds activate mapk and inhibit akt pathways to induce apoptosis in ma 10 mouse leydig tumor cells |
| topic | Akt dimethylarsenic acid Leydig tumor cell MAPK ROS sodium arsenite |
| url | https://doi.org/10.1002/cam4.5068 |
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