Toward measurements of absolute membrane potential in Bacillus subtilis using fluorescence lifetime
Membrane potential (MP) changes can provide a simple readout of bacterial functional and metabolic state or stress levels. While several optical methods exist for measuring fast changes in MP in excitable cells, there is a dearth of such methods for absolute and precise measurements of steady-state...
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Main Authors: | Debjit Roy, Xavier Michalet, Evan W. Miller, Kiran Bharadwaj, Shimon Weiss |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2025-03-01
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Series: | Biophysical Reports |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2667074725000011 |
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