Pine Rhizosphere Soil Microorganisms Enhance the Growth and Resistance of <i>Pinus massoniana</i> Against Nematode Infection
Pine wilt disease, caused by <i>Bursaphelenchus xylophilus</i>, poses severe ecological and economic threats to coniferous forests. This study isolated two fungal (<i>Arthropsis hispanica</i>, <i>Penicillium sclerotiorum</i>) and two bacterial (<i>Bacillus a...
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| Language: | English |
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MDPI AG
2025-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/13/4/790 |
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| author | Jiacheng Zhu Chenxi Deng Yichi Zhang Manman Liu Guoying Zhou Junang Liu |
| author_facet | Jiacheng Zhu Chenxi Deng Yichi Zhang Manman Liu Guoying Zhou Junang Liu |
| author_sort | Jiacheng Zhu |
| collection | DOAJ |
| description | Pine wilt disease, caused by <i>Bursaphelenchus xylophilus</i>, poses severe ecological and economic threats to coniferous forests. This study isolated two fungal (<i>Arthropsis hispanica</i>, <i>Penicillium sclerotiorum</i>) and two bacterial (<i>Bacillus amyloliquefaciens</i>, <i>Enterobacter hormaechei</i>) strains from <i>Pinus massoniana</i> rhizospheres, evaluating their biocontrol potential against pine wood nematodes. Molecular characterization confirmed strain identities. In vitro assays demonstrated that combined fermentation filtrates of CSX134+CSZ71 and CSX60+CSZ71 significantly enhanced plant growth parameters (height, biomass) and root-associated soil enzyme activities (urease, acid phosphatase) in <i>P. massoniana</i>. Treated plants exhibited elevated defense enzyme activities and upregulated defense-related gene expression. The treatments achieved 75.07% and 69.65% nematode control efficacy, respectively, compared to controls. These findings highlight the potential of microbial consortia in activating systemic resistance and suppressing pine wilt disease through the dual mechanisms of growth promotion and defense induction. |
| format | Article |
| id | doaj-art-50ae3d816cd042d4a0eb65be7364666b |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-50ae3d816cd042d4a0eb65be7364666b2025-08-20T03:13:32ZengMDPI AGMicroorganisms2076-26072025-03-0113479010.3390/microorganisms13040790Pine Rhizosphere Soil Microorganisms Enhance the Growth and Resistance of <i>Pinus massoniana</i> Against Nematode InfectionJiacheng Zhu0Chenxi Deng1Yichi Zhang2Manman Liu3Guoying Zhou4Junang Liu5Key Laboratory of National Forestry and Grassland Administration on Control of Artificial Forest Diseases and Pests in South China, Central South University of Forestry and Technology, Changsha 410004, ChinaHunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Central South University of Forestry and Technology, Changsha 410004, ChinaKey Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Central South University of Forestry and Technology, Changsha 410004, ChinaKey Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Central South University of Forestry and Technology, Changsha 410004, ChinaKey Laboratory of National Forestry and Grassland Administration on Control of Artificial Forest Diseases and Pests in South China, Central South University of Forestry and Technology, Changsha 410004, ChinaKey Laboratory of National Forestry and Grassland Administration on Control of Artificial Forest Diseases and Pests in South China, Central South University of Forestry and Technology, Changsha 410004, ChinaPine wilt disease, caused by <i>Bursaphelenchus xylophilus</i>, poses severe ecological and economic threats to coniferous forests. This study isolated two fungal (<i>Arthropsis hispanica</i>, <i>Penicillium sclerotiorum</i>) and two bacterial (<i>Bacillus amyloliquefaciens</i>, <i>Enterobacter hormaechei</i>) strains from <i>Pinus massoniana</i> rhizospheres, evaluating their biocontrol potential against pine wood nematodes. Molecular characterization confirmed strain identities. In vitro assays demonstrated that combined fermentation filtrates of CSX134+CSZ71 and CSX60+CSZ71 significantly enhanced plant growth parameters (height, biomass) and root-associated soil enzyme activities (urease, acid phosphatase) in <i>P. massoniana</i>. Treated plants exhibited elevated defense enzyme activities and upregulated defense-related gene expression. The treatments achieved 75.07% and 69.65% nematode control efficacy, respectively, compared to controls. These findings highlight the potential of microbial consortia in activating systemic resistance and suppressing pine wilt disease through the dual mechanisms of growth promotion and defense induction.https://www.mdpi.com/2076-2607/13/4/790pine wilt diseasesoil microorganismsgrowth-promotingdefense response mechanisms |
| spellingShingle | Jiacheng Zhu Chenxi Deng Yichi Zhang Manman Liu Guoying Zhou Junang Liu Pine Rhizosphere Soil Microorganisms Enhance the Growth and Resistance of <i>Pinus massoniana</i> Against Nematode Infection Microorganisms pine wilt disease soil microorganisms growth-promoting defense response mechanisms |
| title | Pine Rhizosphere Soil Microorganisms Enhance the Growth and Resistance of <i>Pinus massoniana</i> Against Nematode Infection |
| title_full | Pine Rhizosphere Soil Microorganisms Enhance the Growth and Resistance of <i>Pinus massoniana</i> Against Nematode Infection |
| title_fullStr | Pine Rhizosphere Soil Microorganisms Enhance the Growth and Resistance of <i>Pinus massoniana</i> Against Nematode Infection |
| title_full_unstemmed | Pine Rhizosphere Soil Microorganisms Enhance the Growth and Resistance of <i>Pinus massoniana</i> Against Nematode Infection |
| title_short | Pine Rhizosphere Soil Microorganisms Enhance the Growth and Resistance of <i>Pinus massoniana</i> Against Nematode Infection |
| title_sort | pine rhizosphere soil microorganisms enhance the growth and resistance of i pinus massoniana i against nematode infection |
| topic | pine wilt disease soil microorganisms growth-promoting defense response mechanisms |
| url | https://www.mdpi.com/2076-2607/13/4/790 |
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