In vivo MRI characterization of progressive cardiac dysfunction in the mdx mouse model of muscular dystrophy.
<h4>Aims</h4>The mdx mouse has proven to be useful in understanding the cardiomyopathy that frequently occurs in muscular dystrophy patients. Here we employed a comprehensive array of clinically relevant in vivo MRI techniques to identify early markers of cardiac dysfunction and follow d...
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Public Library of Science (PLoS)
2012-01-01
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| author | Daniel J Stuckey Carolyn A Carr Patrizia Camelliti Damian J Tyler Kay E Davies Kieran Clarke |
| author_facet | Daniel J Stuckey Carolyn A Carr Patrizia Camelliti Damian J Tyler Kay E Davies Kieran Clarke |
| author_sort | Daniel J Stuckey |
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| description | <h4>Aims</h4>The mdx mouse has proven to be useful in understanding the cardiomyopathy that frequently occurs in muscular dystrophy patients. Here we employed a comprehensive array of clinically relevant in vivo MRI techniques to identify early markers of cardiac dysfunction and follow disease progression in the hearts of mdx mice.<h4>Methods and results</h4>Serial measurements of cardiac morphology and function were made in the same group of mdx mice and controls (housed in a non-SPF facility) using MRI at 1, 3, 6, 9 and 12 months after birth. Left ventricular (LV) and right ventricular (RV) systolic and diastolic function, response to dobutamine stress and myocardial fibrosis were assessed. RV dysfunction preceded LV dysfunction, with RV end systolic volumes increased and RV ejection fractions reduced at 3 months of age. LV ejection fractions were reduced at 12 months, compared with controls. An abnormal response to dobutamine stress was identified in the RV of mdx mice as early as 1 month. Late-gadolinium-enhanced MRI identified increased levels of myocardial fibrosis in 6, 9 and 12-month-old mdx mice, the extent of fibrosis correlating with the degree of cardiac remodeling and hypertrophy.<h4>Conclusions</h4>MRI could identify cardiac abnormalities in the RV of mdx mice as young as 1 month, and detected myocardial fibrosis at 6 months. We believe these to be the earliest MRI measurements of cardiac function reported for any mice, and the first use of late-gadolinium-enhancement in a mouse model of congenital cardiomyopathy. These techniques offer a sensitive and clinically relevant in vivo method for assessment of cardiomyopathy caused by muscular dystrophy and other diseases. |
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| language | English |
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| spelling | doaj-art-5097eac8e4d040858df642d015ace3cb2025-08-20T02:05:32ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0171e2856910.1371/journal.pone.0028569In vivo MRI characterization of progressive cardiac dysfunction in the mdx mouse model of muscular dystrophy.Daniel J StuckeyCarolyn A CarrPatrizia CamellitiDamian J TylerKay E DaviesKieran Clarke<h4>Aims</h4>The mdx mouse has proven to be useful in understanding the cardiomyopathy that frequently occurs in muscular dystrophy patients. Here we employed a comprehensive array of clinically relevant in vivo MRI techniques to identify early markers of cardiac dysfunction and follow disease progression in the hearts of mdx mice.<h4>Methods and results</h4>Serial measurements of cardiac morphology and function were made in the same group of mdx mice and controls (housed in a non-SPF facility) using MRI at 1, 3, 6, 9 and 12 months after birth. Left ventricular (LV) and right ventricular (RV) systolic and diastolic function, response to dobutamine stress and myocardial fibrosis were assessed. RV dysfunction preceded LV dysfunction, with RV end systolic volumes increased and RV ejection fractions reduced at 3 months of age. LV ejection fractions were reduced at 12 months, compared with controls. An abnormal response to dobutamine stress was identified in the RV of mdx mice as early as 1 month. Late-gadolinium-enhanced MRI identified increased levels of myocardial fibrosis in 6, 9 and 12-month-old mdx mice, the extent of fibrosis correlating with the degree of cardiac remodeling and hypertrophy.<h4>Conclusions</h4>MRI could identify cardiac abnormalities in the RV of mdx mice as young as 1 month, and detected myocardial fibrosis at 6 months. We believe these to be the earliest MRI measurements of cardiac function reported for any mice, and the first use of late-gadolinium-enhancement in a mouse model of congenital cardiomyopathy. These techniques offer a sensitive and clinically relevant in vivo method for assessment of cardiomyopathy caused by muscular dystrophy and other diseases.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0028569&type=printable |
| spellingShingle | Daniel J Stuckey Carolyn A Carr Patrizia Camelliti Damian J Tyler Kay E Davies Kieran Clarke In vivo MRI characterization of progressive cardiac dysfunction in the mdx mouse model of muscular dystrophy. PLoS ONE |
| title | In vivo MRI characterization of progressive cardiac dysfunction in the mdx mouse model of muscular dystrophy. |
| title_full | In vivo MRI characterization of progressive cardiac dysfunction in the mdx mouse model of muscular dystrophy. |
| title_fullStr | In vivo MRI characterization of progressive cardiac dysfunction in the mdx mouse model of muscular dystrophy. |
| title_full_unstemmed | In vivo MRI characterization of progressive cardiac dysfunction in the mdx mouse model of muscular dystrophy. |
| title_short | In vivo MRI characterization of progressive cardiac dysfunction in the mdx mouse model of muscular dystrophy. |
| title_sort | in vivo mri characterization of progressive cardiac dysfunction in the mdx mouse model of muscular dystrophy |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0028569&type=printable |
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