BnDREB1 confers cadmium tolerance in ramie
Abstract Cadmium (Cd) is a toxic heavy metal whose contamination in soil threatens food safety, agricultural production, and human health. To date, phytoremediation is a low-cost and environmentally friendly method for eliminating Cd contamination. In this study, we report a gene from ramie (Boehmer...
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Nature Portfolio
2025-04-01
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| Online Access: | https://doi.org/10.1038/s41598-025-96051-1 |
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| author | Xiaoyang Zhang Mingyu Shao Wenxian Peng Hongyue Qu Xinran Han Hucheng Xing |
| author_facet | Xiaoyang Zhang Mingyu Shao Wenxian Peng Hongyue Qu Xinran Han Hucheng Xing |
| author_sort | Xiaoyang Zhang |
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| description | Abstract Cadmium (Cd) is a toxic heavy metal whose contamination in soil threatens food safety, agricultural production, and human health. To date, phytoremediation is a low-cost and environmentally friendly method for eliminating Cd contamination. In this study, we report a gene from ramie (Boehmeria nivea) that encodes a dehydration responsive element binding (DREB) factor associated with plant tolerance to Cd, namely BnDREB1. The open reading frame of BnDREB1 comprises 873 bp encoding 290 amino acids and includes a characteristic AP2 domain. Its cloned promoter sequence contains various hormone and stress responsive elements. Quantitative RT-PCR analysis showed that BnDREB1 is expressed in different organs of ramie. Treatments with polyethylene glycol (PEG), abscisic acid (ABA), and Cd upregulated the expression of BnDREB1. Confocal microscopic analysis revealed that BnDREB1 is mainly localized in the nucleus. Overexpression of BnDREB1 in Arabidopsis thaliana increased the tolerance of transgenic plants to Cd, thereby protecting plant growth from its toxicity. Biochemical analysis revealed that overexpression of BnDREB1 reduced the levels of Cd induced malonaldehyde and hydrogen peroxide, inhibited the reduction of Cd caused soluble protein contents, increased the Cd accumulation, and enhanced Cd translocation in transgenic plants. Taken together, these findings suggest that BnDREB1 is an appropriate candidate gene for phytoremediation of Cd-contaminated soil . |
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| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
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| spelling | doaj-art-508a37ac2b4943a1a9aa93e4d98d4c012025-08-20T02:25:40ZengNature PortfolioScientific Reports2045-23222025-04-0115111310.1038/s41598-025-96051-1BnDREB1 confers cadmium tolerance in ramieXiaoyang Zhang0Mingyu Shao1Wenxian Peng2Hongyue Qu3Xinran Han4Hucheng Xing5Ramie Research Institute of Hunan Agricultural UniversityRamie Research Institute of Hunan Agricultural UniversityRamie Research Institute of Hunan Agricultural UniversityRamie Research Institute of Hunan Agricultural UniversityRamie Research Institute of Hunan Agricultural UniversityRamie Research Institute of Hunan Agricultural UniversityAbstract Cadmium (Cd) is a toxic heavy metal whose contamination in soil threatens food safety, agricultural production, and human health. To date, phytoremediation is a low-cost and environmentally friendly method for eliminating Cd contamination. In this study, we report a gene from ramie (Boehmeria nivea) that encodes a dehydration responsive element binding (DREB) factor associated with plant tolerance to Cd, namely BnDREB1. The open reading frame of BnDREB1 comprises 873 bp encoding 290 amino acids and includes a characteristic AP2 domain. Its cloned promoter sequence contains various hormone and stress responsive elements. Quantitative RT-PCR analysis showed that BnDREB1 is expressed in different organs of ramie. Treatments with polyethylene glycol (PEG), abscisic acid (ABA), and Cd upregulated the expression of BnDREB1. Confocal microscopic analysis revealed that BnDREB1 is mainly localized in the nucleus. Overexpression of BnDREB1 in Arabidopsis thaliana increased the tolerance of transgenic plants to Cd, thereby protecting plant growth from its toxicity. Biochemical analysis revealed that overexpression of BnDREB1 reduced the levels of Cd induced malonaldehyde and hydrogen peroxide, inhibited the reduction of Cd caused soluble protein contents, increased the Cd accumulation, and enhanced Cd translocation in transgenic plants. Taken together, these findings suggest that BnDREB1 is an appropriate candidate gene for phytoremediation of Cd-contaminated soil .https://doi.org/10.1038/s41598-025-96051-1ArabidopsisBoehmeria niveaDREBTranscription factorCadmium stressTransgenic plant |
| spellingShingle | Xiaoyang Zhang Mingyu Shao Wenxian Peng Hongyue Qu Xinran Han Hucheng Xing BnDREB1 confers cadmium tolerance in ramie Scientific Reports Arabidopsis Boehmeria nivea DREB Transcription factor Cadmium stress Transgenic plant |
| title | BnDREB1 confers cadmium tolerance in ramie |
| title_full | BnDREB1 confers cadmium tolerance in ramie |
| title_fullStr | BnDREB1 confers cadmium tolerance in ramie |
| title_full_unstemmed | BnDREB1 confers cadmium tolerance in ramie |
| title_short | BnDREB1 confers cadmium tolerance in ramie |
| title_sort | bndreb1 confers cadmium tolerance in ramie |
| topic | Arabidopsis Boehmeria nivea DREB Transcription factor Cadmium stress Transgenic plant |
| url | https://doi.org/10.1038/s41598-025-96051-1 |
| work_keys_str_mv | AT xiaoyangzhang bndreb1conferscadmiumtoleranceinramie AT mingyushao bndreb1conferscadmiumtoleranceinramie AT wenxianpeng bndreb1conferscadmiumtoleranceinramie AT hongyuequ bndreb1conferscadmiumtoleranceinramie AT xinranhan bndreb1conferscadmiumtoleranceinramie AT huchengxing bndreb1conferscadmiumtoleranceinramie |