One-Step Labeling Based on Eu-MOFs to Develop Fluorescence Side-Flow Immunoassay for AFB1 Detection in Corn
Lateral flow immunoassay (LFIA) is a promising tool for rapid detection in the field of agricultural product analysis due to its advantages of cost-effectiveness and operational simplicity. In this work, Eu metal–organic frameworks (MOFs) were introduced to LFIA as a rapid detection method character...
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MDPI AG
2025-05-01
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| Series: | Biosensors |
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| author | Yinjun Li Hua Ding Ziyu Wang Zewei Luo Xitian Peng |
| author_facet | Yinjun Li Hua Ding Ziyu Wang Zewei Luo Xitian Peng |
| author_sort | Yinjun Li |
| collection | DOAJ |
| description | Lateral flow immunoassay (LFIA) is a promising tool for rapid detection in the field of agricultural product analysis due to its advantages of cost-effectiveness and operational simplicity. In this work, Eu metal–organic frameworks (MOFs) were introduced to LFIA as a rapid detection method characterized by high stability and low interference. Key research objectives included strong fluorescence, ease of labeling, and the utilization of fluorescent probes. Eu-MOFs were synthesized in one step via the hydrothermal method, exhibiting a fluorescence lifetime of 163 μs and spherical particles with diameters ranging from 250 to 400 nm. These conditions fulfill the characteristics and requirements of LFIA. Eu-MOFs exploit the porous nature of MOFs to mitigate the drawbacks associated with complex crosslinking agents. This enables antibody proteins to be cross-linked merely upon contact, thereby simplifying the detection process. A time-resolved LFIA method was developed utilizing Eu-MOFs for the detection of aflatoxin B1 (AFB1) in corn, achieving a limit of detection (LOD, IC10) of 0.149 ng/mL. The accuracy and reliability of the Eu-MOFs-LFIA method were validated through comparisons with spiked concentrations during spiking and blind sample analyses, with verification conducted using ultra-high-performance liquid chromatography mass spectrometry (UPLC-MS). Furthermore, testing of real samples demonstrated that the Eu-MOFs-LFIA method can effectively facilitate rapid detection of AFB1 in corn. |
| format | Article |
| id | doaj-art-50862f0de1d54009a85559e17aaa0b5a |
| institution | Kabale University |
| issn | 2079-6374 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
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| series | Biosensors |
| spelling | doaj-art-50862f0de1d54009a85559e17aaa0b5a2025-08-20T03:47:48ZengMDPI AGBiosensors2079-63742025-05-0115531310.3390/bios15050313One-Step Labeling Based on Eu-MOFs to Develop Fluorescence Side-Flow Immunoassay for AFB1 Detection in CornYinjun Li0Hua Ding1Ziyu Wang2Zewei Luo3Xitian Peng4Institute of Agricultural Quality Standards and Testing Technology Research, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Nutritional Quality and Safety of Agro Products, Wuhan 430064, ChinaInstitute of Agricultural Quality Standards and Testing Technology Research, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Nutritional Quality and Safety of Agro Products, Wuhan 430064, ChinaResearch Center of Analytical Instrumentation, School of Mechanical Engineering, Sichuan University, Chengdu 610065, ChinaResearch Center of Analytical Instrumentation, School of Mechanical Engineering, Sichuan University, Chengdu 610065, ChinaInstitute of Agricultural Quality Standards and Testing Technology Research, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Nutritional Quality and Safety of Agro Products, Wuhan 430064, ChinaLateral flow immunoassay (LFIA) is a promising tool for rapid detection in the field of agricultural product analysis due to its advantages of cost-effectiveness and operational simplicity. In this work, Eu metal–organic frameworks (MOFs) were introduced to LFIA as a rapid detection method characterized by high stability and low interference. Key research objectives included strong fluorescence, ease of labeling, and the utilization of fluorescent probes. Eu-MOFs were synthesized in one step via the hydrothermal method, exhibiting a fluorescence lifetime of 163 μs and spherical particles with diameters ranging from 250 to 400 nm. These conditions fulfill the characteristics and requirements of LFIA. Eu-MOFs exploit the porous nature of MOFs to mitigate the drawbacks associated with complex crosslinking agents. This enables antibody proteins to be cross-linked merely upon contact, thereby simplifying the detection process. A time-resolved LFIA method was developed utilizing Eu-MOFs for the detection of aflatoxin B1 (AFB1) in corn, achieving a limit of detection (LOD, IC10) of 0.149 ng/mL. The accuracy and reliability of the Eu-MOFs-LFIA method were validated through comparisons with spiked concentrations during spiking and blind sample analyses, with verification conducted using ultra-high-performance liquid chromatography mass spectrometry (UPLC-MS). Furthermore, testing of real samples demonstrated that the Eu-MOFs-LFIA method can effectively facilitate rapid detection of AFB1 in corn.https://www.mdpi.com/2079-6374/15/5/313lateral flow immunoassaymetal–organic frameworksaflatoxin B1rapid detection |
| spellingShingle | Yinjun Li Hua Ding Ziyu Wang Zewei Luo Xitian Peng One-Step Labeling Based on Eu-MOFs to Develop Fluorescence Side-Flow Immunoassay for AFB1 Detection in Corn Biosensors lateral flow immunoassay metal–organic frameworks aflatoxin B1 rapid detection |
| title | One-Step Labeling Based on Eu-MOFs to Develop Fluorescence Side-Flow Immunoassay for AFB1 Detection in Corn |
| title_full | One-Step Labeling Based on Eu-MOFs to Develop Fluorescence Side-Flow Immunoassay for AFB1 Detection in Corn |
| title_fullStr | One-Step Labeling Based on Eu-MOFs to Develop Fluorescence Side-Flow Immunoassay for AFB1 Detection in Corn |
| title_full_unstemmed | One-Step Labeling Based on Eu-MOFs to Develop Fluorescence Side-Flow Immunoassay for AFB1 Detection in Corn |
| title_short | One-Step Labeling Based on Eu-MOFs to Develop Fluorescence Side-Flow Immunoassay for AFB1 Detection in Corn |
| title_sort | one step labeling based on eu mofs to develop fluorescence side flow immunoassay for afb1 detection in corn |
| topic | lateral flow immunoassay metal–organic frameworks aflatoxin B1 rapid detection |
| url | https://www.mdpi.com/2079-6374/15/5/313 |
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