Exploring the role of OIP5-AS1 in the mechanisms of delayed fracture healing: functional insights and clinical implications
Abstract Objective Fracture is a common traumatic disease and there is a risk of delayed healing after fracture occurs. This study aimed to explore the regulatory roles and clinical implications of OIP5-AS1 in delayed fracture healing. Methods The study included 80 normal fracture healing patients a...
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BMC
2025-01-01
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| Series: | Journal of Orthopaedic Surgery and Research |
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| Online Access: | https://doi.org/10.1186/s13018-024-05428-x |
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| author | Yusen Hu Meini Cen Yi Hu |
| author_facet | Yusen Hu Meini Cen Yi Hu |
| author_sort | Yusen Hu |
| collection | DOAJ |
| description | Abstract Objective Fracture is a common traumatic disease and there is a risk of delayed healing after fracture occurs. This study aimed to explore the regulatory roles and clinical implications of OIP5-AS1 in delayed fracture healing. Methods The study included 80 normal fracture healing patients and 80 delayed fracture healing patients. RT-qPCR was used to assess the levels of OIP5-AS1 and miR-7-5p in the serum of patients and MC3T3-E1 cells. The ROC curve was utilized to evaluate the predictive value of OIP5-AS1 for delayed fracture healing. Dual-luciferase reporter assays and RIP assays were conducted to validate the interaction between OIP5-AS1 and miR-7-5p. Cell viability and apoptosis were determined by CCK-8 and flow cytometry. Results OIP5-AS1 was abnormally elevated in the serum of delayed fracture healing patients, and OIP5-AS1 had a predictive value for delayed fracture healing. Cell experiments demonstrated that overexpression of OIP5-AS1 significantly inhibited osteogenic differentiation, reduced cell viability, and stimulated apoptosis. miR-7-5p was identified as the target miRNA of OIP5-AS1 and was negatively regulated by OIP5-AS1. Furthermore, transfecting with miR-7-5p mimic significantly alleviated the inhibitory effect of OIP5-AS1 on osteoblast activity. Conclusion OIP5-AS1 was identified as a potential biomarker for predicting delayed fracture healing. Additionally, it could inhibit fracture healing through the sponge effect on miR-7-5p. |
| format | Article |
| id | doaj-art-507a96435991455cb704f4d122f58bb7 |
| institution | Kabale University |
| issn | 1749-799X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj-art-507a96435991455cb704f4d122f58bb72025-01-12T12:32:31ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2025-01-012011810.1186/s13018-024-05428-xExploring the role of OIP5-AS1 in the mechanisms of delayed fracture healing: functional insights and clinical implicationsYusen Hu0Meini Cen1Yi Hu2Department of Orthopaedics, The First People’s Hopital of ChangzhouDepartment of Rehabilitation Medicine, The Affiliated Hospital of Youjiang Medical University for Nationalities Department of Orthopaedics, Changde Hospital, Xiangya School of Medicine, Central South University (The First People’s Hospital of Changde City)Abstract Objective Fracture is a common traumatic disease and there is a risk of delayed healing after fracture occurs. This study aimed to explore the regulatory roles and clinical implications of OIP5-AS1 in delayed fracture healing. Methods The study included 80 normal fracture healing patients and 80 delayed fracture healing patients. RT-qPCR was used to assess the levels of OIP5-AS1 and miR-7-5p in the serum of patients and MC3T3-E1 cells. The ROC curve was utilized to evaluate the predictive value of OIP5-AS1 for delayed fracture healing. Dual-luciferase reporter assays and RIP assays were conducted to validate the interaction between OIP5-AS1 and miR-7-5p. Cell viability and apoptosis were determined by CCK-8 and flow cytometry. Results OIP5-AS1 was abnormally elevated in the serum of delayed fracture healing patients, and OIP5-AS1 had a predictive value for delayed fracture healing. Cell experiments demonstrated that overexpression of OIP5-AS1 significantly inhibited osteogenic differentiation, reduced cell viability, and stimulated apoptosis. miR-7-5p was identified as the target miRNA of OIP5-AS1 and was negatively regulated by OIP5-AS1. Furthermore, transfecting with miR-7-5p mimic significantly alleviated the inhibitory effect of OIP5-AS1 on osteoblast activity. Conclusion OIP5-AS1 was identified as a potential biomarker for predicting delayed fracture healing. Additionally, it could inhibit fracture healing through the sponge effect on miR-7-5p.https://doi.org/10.1186/s13018-024-05428-xOIP5-AS1miR-7-5pDelayed fracture healingBiomarker |
| spellingShingle | Yusen Hu Meini Cen Yi Hu Exploring the role of OIP5-AS1 in the mechanisms of delayed fracture healing: functional insights and clinical implications Journal of Orthopaedic Surgery and Research OIP5-AS1 miR-7-5p Delayed fracture healing Biomarker |
| title | Exploring the role of OIP5-AS1 in the mechanisms of delayed fracture healing: functional insights and clinical implications |
| title_full | Exploring the role of OIP5-AS1 in the mechanisms of delayed fracture healing: functional insights and clinical implications |
| title_fullStr | Exploring the role of OIP5-AS1 in the mechanisms of delayed fracture healing: functional insights and clinical implications |
| title_full_unstemmed | Exploring the role of OIP5-AS1 in the mechanisms of delayed fracture healing: functional insights and clinical implications |
| title_short | Exploring the role of OIP5-AS1 in the mechanisms of delayed fracture healing: functional insights and clinical implications |
| title_sort | exploring the role of oip5 as1 in the mechanisms of delayed fracture healing functional insights and clinical implications |
| topic | OIP5-AS1 miR-7-5p Delayed fracture healing Biomarker |
| url | https://doi.org/10.1186/s13018-024-05428-x |
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